{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T11:10:23Z","timestamp":1788952223700,"version":"build-2803163510"},"reference-count":45,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T00:00:00Z","timestamp":1788912000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:p>\n                    Ulcerative colitis (UC) involves epithelial injury, gut microbial dysbiosis, disturbed microbial metabolism, and mucosal inflammation. This study evaluated the protective effect of\n                    <jats:italic>Schisandra chinensis<\/jats:italic>\n                    (SC) against dextran sulfate sodium (DSS)-induced UC and investigated the underlying microbiota\u2013metabolite\u2013host signaling mechanism. UPLC-Q-TOF-MS\/MS identified 38 constituents in SC, predominantly lignans, organic and phenolic acids, and terpenoids. SC reduced body weight loss, disease activity, colon shortening, mucosal and histological injury, and pro-inflammatory cytokine levels, while partially restoring claudin-1 and occludin expression. Metagenomic analysis showed that SC restructured the DSS-disrupted microbiota, enriched\n                    <jats:italic>Parabacteroides goldsteinii<\/jats:italic>\n                    , and increased the predicted abundance of pathways related to valine, leucine, and isoleucine degradation. Untargeted metabolomics and targeted fatty acid analysis showed lower fecal leucine and higher isovalerate levels after SC treatment. Whole-colon transcriptomic and western blot analyses further demonstrated that SC suppressed the mTOR\/HIF-1\u03b1\/STAT3\/IL-17 inflammatory program in colonic tissue. Antibiotic pretreatment weakened several protective effects of SC, whereas\n                    <jats:italic>P. goldsteinii<\/jats:italic>\n                    or sodium isovalerate administration partially recapitulated its anti-inflammatory and barrier-restorative effects. Conversely, the mTOR activator MHY1485 attenuated SC-mediated protection. These findings indicate that the protective effects of SC are associated with remodeling of a\n                    <jats:italic>P. goldsteinii<\/jats:italic>\n                    -related leucine\u2013isovalerate metabolic network and attenuation of colonic mTOR\/HIF-1\u03b1\/STAT3\/IL-17 signaling. This study provides a microbiota-centered mechanistic basis for the therapeutic potential of SC in UC.\n                  <\/jats:p>","DOI":"10.3389\/fmicb.2026.1941347","type":"journal-article","created":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T10:16:55Z","timestamp":1788949015000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Schisandra chinensis alleviates dextran sulfate sodium-induced colitis in association with remodeling of a Parabacteroides goldsteinii-related leucine\u2013isovalerate metabolic axis and attenuation of mTOR\/HIF-1\u03b1\/IL-17 signaling"],"prefix":"10.3389","volume":"17","author":[{"given":"Jiantao","family":"Song","sequence":"first","affiliation":[{"name":"Department of Pharmacy, The First People's Hospital of Changzhou","place":["Changzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongfei","family":"Kan","sequence":"additional","affiliation":[{"name":"College of Pharmacy, Nanjing University of Chinese Medicine","place":["Nanjing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huaijie","family":"Jin","sequence":"additional","affiliation":[{"name":"College of Pharmacy, Nanjing University of Chinese Medicine","place":["Nanjing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lin","family":"Yue","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, The First People's Hospital of Changzhou","place":["Changzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhouyi","family":"Qian","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, The First People's Hospital of Changzhou","place":["Changzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bo","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Pharmacy, Nanjing University of Chinese Medicine","place":["Nanjing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zongling","family":"Xia","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, The First People's Hospital of Changzhou","place":["Changzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanan","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery, Shanghai Changhai Hospital, Naval Medical University","place":["Shanghai, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiuba","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Pharmacy, Nanjing University of Chinese Medicine","place":["Nanjing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,9,9]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"G459","DOI":"10.1152\/ajpgi.00193.2025","article-title":"The gut microbiota metabolite isovalerate enhances the epithelial barrier function in cell monolayers derived from porcine ileum organoids","volume":"330","author":"Beaumont","year":"2026","journal-title":"Am. 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