{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T06:04:23Z","timestamp":1789452263593,"version":"build-2803163510"},"reference-count":52,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T00:00:00Z","timestamp":1789430400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002383","name":"King Saud University","doi-asserted-by":"publisher","award":["ORF-2026-1876"],"award-info":[{"award-number":["ORF-2026-1876"]}],"id":[{"id":"10.13039\/501100002383","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"\n Background<\/jats:title>\n \n The rise of multidrug-resistant\n Pseudomonas aeruginosa<\/jats:italic>\n has heightened interest in microbiome-based anti-virulence strategies that mitigate pathogenicity without directly affecting bacterial survival. This study examined whether the fermentation of Arabic gum and baobab by probiotic bacteria produces metabolites that reduce\n P. aeruginosa<\/jats:italic>\n virulence.\n <\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n \n The carbohydrate-active enzyme profiles of\n Bifidobacterium longum<\/jats:italic>\n ,\n Lactiplantibacillus plantarum<\/jats:italic>\n , and\n P. aeruginosa<\/jats:italic>\n were compared to predict their ability to utilize plant glycans. Probiotic growth, viable counts, and acidification were measured following supplementation with Arabic gum, baobab, or glucose. The effects of untreated, neutralized, and pH-matched cell-free supernatants on\n P. aeruginosa<\/jats:italic>\n growth, biofilm formation, adhesion, and twitching motility were evaluated.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n L. plantarum<\/jats:italic>\n and\n B. longum<\/jats:italic>\n exhibited 54 and 53 glycoside hydrolases, respectively, compared to 29 in\n P. aeruginosa<\/jats:italic>\n , suggesting a superior probiotic capacity for plant-glycan utilization. Arabic gum at 2% significantly enhanced\n B. longum<\/jats:italic>\n viability by approximately 0.27 log\n 10<\/jats:sub>\n CFU mL\n \u22121<\/jats:sup>\n (\n P<\/jats:italic>\n = 0.0058) and resulted in the greatest pH reduction, whereas glucose increased\n L. plantarum<\/jats:italic>\n growth by approximately 0.58 log\n 10<\/jats:sub>\n CFU mL\n \u22121<\/jats:sup>\n (\n P<\/jats:italic>\n = 0.0019). Supernatants derived from Arabic gum exhibited the strongest activity, nearly completely inhibiting biofilm formation, reducing adhesion by approximately 54%, and significantly suppressing twitching motility. Baobab demonstrated measurable but generally weaker prebiotic and anti-virulence effects. These findings indicate that the biological effects were contingent on the fermented substrate and the resulting metabolite profile. Neutralization reduced several inhibitory effects, while pH-matched controls largely replicated the growth suppression observed with the corresponding CFS. This indicates that fermentation-associated acidification was a primary determinant of antibacterial activity. The residual activity observed in certain neutralized CFS preparations suggests that additional non-acidic factors may contribute under specific conditions.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion<\/jats:title>\n \n Genome-informed glycan-utilization predictions identified substrates capable of producing probiotic metabolites that attenuate multiple\n P. aeruginosa<\/jats:italic>\n virulence phenotypes. Arabic gum shows particular promise for microbiome-based control of multidrug-resistant\n P. aeruginosa.<\/jats:italic>\n <\/jats:p>\n <\/jats:sec>","DOI":"10.3389\/fmicb.2026.1948465","type":"journal-article","created":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T05:33:51Z","timestamp":1789450431000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Genome-guided prebiotic fermentation generates anti-virulence metabolites against Pseudomonas aeruginosa"],"prefix":"10.3389","volume":"17","author":[{"given":"Rasha","family":"Elsayim","sequence":"first","affiliation":[{"name":"Department of Botany and Microbiology, College of Science, King Saud University","place":["Riyadh, Saudi Arabia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Monerah S. 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