{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T15:05:42Z","timestamp":1789657542681,"version":"4.0.1"},"reference-count":48,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T00:00:00Z","timestamp":1789603200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Science and Technology Program of the Division and City, Xinjiang","award":["2025GG1201"],"award-info":[{"award-number":["2025GG1201"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32570039"],"award-info":[{"award-number":["32570039"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32270060"],"award-info":[{"award-number":["32270060"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32371730"],"award-info":[{"award-number":["32371730"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:p>\n                    Watermelon Fusarium wilt is a severe soil-borne disease that threatens continuous watermelon production. Effective disease control depends on the differentiation of pathogen races, the accumulation of soil-borne inoculum, and disturbances in the soil bacterial community caused by continuous cropping. In this study, a pathogenic fungus was isolated from diseased watermelon plants in a continuously cropped field in Dongtai, Jiangsu Province, China. Morphological characterization, multigene phylogenetic analysis, and pathogenicity assays revealed that the pathogenic isolate belongs to\n                    <jats:italic>Fusarium oxysporum<\/jats:italic>\n                    f. sp.\n                    <jats:italic>niveum<\/jats:italic>\n                    (FON). The biocontrol efficacy of the predatory myxobacteria\n                    <jats:italic>Corallococcus<\/jats:italic>\n                    sp. EGB against FON was also evaluated under field conditions in Dongtai. Strain EGB maintained high target gene copy numbers in soil under consecutive cropping, which in turn reduced the FON target gene copy numbers and thereby lowered the incidence of fusarium wilt. Additionally, EGB application alters the composition of the root-zone soil bacterial community, shifts dominant taxa, and changes diversity patterns in the field experiments. These results indicate that EGB effectively suppresses watermelon Fusarium wilt caused by FON under field conditions, supporting the potential of EGB as a sustainable strategy for managing watermelon Fusarium wilt.\n                  <\/jats:p>","DOI":"10.3389\/fmicb.2026.1950692","type":"journal-article","created":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T14:20:21Z","timestamp":1789654821000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Biocontrol of watermelon Fusarium wilt caused by Fusarium oxysporum f. sp. niveum with Corallococcus sp. EGB"],"prefix":"10.3389","volume":"17","author":[{"given":"Xinjie","family":"Li","sequence":"first","affiliation":[{"name":"Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture and Rural Affairs, College of Life Sciences, Nanjing Agricultural University","place":["Nanjing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingze","family":"Gao","sequence":"additional","affiliation":[{"name":"Nanjing Foreign Language School","place":["Nanjing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhitao","family":"Geng","sequence":"additional","affiliation":[{"name":"Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture and Rural Affairs, College of Life Sciences, Nanjing Agricultural University","place":["Nanjing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Minxin","family":"Hou","sequence":"additional","affiliation":[{"name":"Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture and Rural Affairs, College of Life Sciences, Nanjing Agricultural University","place":["Nanjing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiangmin","family":"Li","sequence":"additional","affiliation":[{"name":"Nanjing Institute of Environmental Sciences, Ministry of Ecology and Environment","place":["Nanjing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhoukun","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture and Rural Affairs, College of Life Sciences, Nanjing Agricultural University","place":["Nanjing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,9,17]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"165","DOI":"10.3389\/fpls.2013.00165","article-title":"The rhizosphere revisited: root microbiomics","volume":"4","author":"Bakker","year":"2013","journal-title":"Front. Plant Sci."},{"key":"ref2","doi-asserted-by":"publisher","first-page":"666","DOI":"10.1007\/s00203-022-03279-w","article-title":"Microorganisms in biological control strategies to manage microbial plant pathogens: a review","volume":"204","author":"Boro","year":"2022","journal-title":"Arch. Microbiol."},{"key":"ref3","doi-asserted-by":"publisher","first-page":"889","DOI":"10.1094\/PDIS.2002.86.8.889","article-title":"Interactions between myxobacteria, plant pathogenic fungi, and biocontrol agents","volume":"86","author":"Bull","year":"2002","journal-title":"Plant Dis."},{"key":"ref4","volume-title":"The Establishment of the Molecular Detection Technique and Basic Identification of Effectors of","author":"Cao","year":"2016"},{"key":"ref5","volume-title":"Fusarium Wilt of Watermelon in Georgia","author":"Dutta","year":"2024"},{"key":"ref6","doi-asserted-by":"publisher","first-page":"1","DOI":"10.13189\/azb.2017.050101","article-title":"Varietal reaction of cucumber (Cucumis sativus L.) germplasm for management of fusarium wilt of cucumber (FWC)","volume":"5","author":"Fareed","year":"2017","journal-title":"Adv. Zool. Bot."},{"key":"ref7","volume-title":"FAOSTAT: Crops and Livestock Products","year":"2026"},{"key":"ref8","doi-asserted-by":"publisher","first-page":"wrae180","DOI":"10.1093\/ismejo\/wrae180","article-title":"Predatory protists impact plant performance by promoting plant growth-promoting rhizobacterial consortia","volume":"18","author":"Guo","year":"2024","journal-title":"ISME J."},{"key":"ref9","doi-asserted-by":"publisher","first-page":"2154","DOI":"10.1098\/rspb.2015.2154","article-title":"Bacterial predator-prey dynamics in microscale patchy landscapes","volume":"283","author":"Hol","year":"2016","journal-title":"Proc. Biol. Sci."},{"key":"ref10","doi-asserted-by":"publisher","first-page":"9735","DOI":"10.3390\/ijms22189735","article-title":"Fusarium oxysporum f. sp. niveum molecular diagnostics past, present and future","volume":"22","author":"Hudson","year":"","journal-title":"Int. J. Mol. Sci."},{"key":"ref11","doi-asserted-by":"publisher","first-page":"822","DOI":"10.3390\/ijms22020822","article-title":"Marker development for differentiation of fusarium Oxysporum f. sp. Niveum race 3 from races 1 and 2","volume":"22","author":"Hudson","year":"","journal-title":"Int. J. Mol. Sci."},{"key":"ref12","doi-asserted-by":"publisher","first-page":"704","DOI":"10.3390\/jof7090704","article-title":"Molecular characterization of laboratory mutants of fusarium oxysporum f. sp. niveum resistant to prothioconazole, a demethylation inhibitor (DMI) fungicide","volume":"7","author":"Hudson","year":"","journal-title":"J. Fungi"},{"key":"ref13","doi-asserted-by":"publisher","first-page":"27","DOI":"10.1094\/PHP-2011-1129-01-DG","article-title":"A diagnostic guide for fusarium wilt of watermelon","volume":"12","author":"Kleczewski","year":"2011","journal-title":"Plant Health Prog."},{"key":"ref14","doi-asserted-by":"publisher","first-page":"10","DOI":"10.1016\/j.biocontrol.2017.04.001","article-title":"Antifungal potential of Corallococcus sp. strain EGB against plant pathogenic fungi","volume":"110","author":"Li","year":"2017","journal-title":"Biol. 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Microbiol."},{"key":"ref24","doi-asserted-by":"publisher","first-page":"1316","DOI":"10.1002\/csc2.20961","article-title":"Geostatistical techniques to account for the heterogeneity of fusarium wilt inoculum distribution in upland cotton field screening studies","volume":"63","author":"Parris","year":"2023","journal-title":"Crop Sci."},{"key":"ref25","doi-asserted-by":"publisher","first-page":"e0219821","DOI":"10.1371\/journal.pone.0219821","article-title":"Genetic and phenotypic diversity of fusarium oxysporum f. sp. niveum populations from watermelon in the southeastern United States","volume":"14","author":"Petkar","year":"2019","journal-title":"PLoS One"},{"key":"ref26","doi-asserted-by":"publisher","first-page":"2665","DOI":"10.1038\/s41396-021-00958-2","article-title":"The soil microbial food web revisited: predatory myxobacteria as keystone taxa?","volume":"15","author":"Petters","year":"2021","journal-title":"ISME J."},{"key":"ref27","doi-asserted-by":"publisher","first-page":"224","DOI":"10.3390\/microorganisms11010224","article-title":"Microbial-based products to control soil-borne pathogens: methods to improve efficacy and to assess impacts on microbiome","volume":"11","author":"Ptaszek","year":"2023","journal-title":"Microorganisms"},{"key":"ref28","doi-asserted-by":"publisher","first-page":"1310","DOI":"10.3390\/agronomy11071310","article-title":"Biology, diversity, detection and management of fusarium oxysporum f. sp. niveum causing vascular wilt disease of watermelon (Citrullus lanatus): a review","volume":"11","author":"Rahman","year":"2021","journal-title":"Agronomy"},{"key":"ref29","volume-title":"Fusarium Wilt (","author":"Roberts","year":"2023"},{"key":"ref30","first-page":"20240105","article-title":"Effect of planting density and variety on growth, yield, and quality of watermelon (Citrullus lanatus) in northwestern Ethiopia","volume":"9","author":"Seid","year":"2024","journal-title":"Open Agric."},{"key":"ref31","doi-asserted-by":"publisher","first-page":"2","DOI":"10.3389\/fmicb.2020.00002","article-title":"The predation strategy of Myxococcus xanthus","volume":"11","author":"Thiery","year":"2020","journal-title":"Front. 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Res."},{"key":"ref35","doi-asserted-by":"publisher","first-page":"334","DOI":"10.21273\/hortsci.47.3.334","article-title":"Identification of resistance to fusarium oxysporum f. sp. niveum race 2 in Citrullus lanatus var. citroides plant introductions","volume":"47","author":"Wechter","year":"2012","journal-title":"HortScience"},{"key":"ref36","doi-asserted-by":"publisher","first-page":"250","DOI":"10.1094\/PHYTO-97-2-0250","article-title":"Pseudomonas biocontrol agents of soilborne pathogens: looking back over 30 years","volume":"97","author":"Weller","year":"2007","journal-title":"Phytopathology"},{"key":"ref37","doi-asserted-by":"publisher","first-page":"312","DOI":"10.1038\/s41579-022-00819-5","article-title":"Trichoderma: a multipurpose, plant-beneficial microorganism for eco-sustainable agriculture","volume":"21","author":"Woo","year":"2023","journal-title":"Nat. Rev. Microbiol."},{"key":"ref38","doi-asserted-by":"publisher","first-page":"5646","DOI":"10.1038\/s41467-023-41247-0","article-title":"Myxobacteria restrain Phytophthora invasion by scavenging thiamine in soybean rhizosphere via outer membrane vesicle-secreted thiaminase I","volume":"14","author":"Xia","year":"2023","journal-title":"Nat. Commun."},{"key":"ref39","doi-asserted-by":"publisher","first-page":"12","DOI":"10.1093\/jhered\/esh076","article-title":"Relationship of PAL activity and transfer of resistance to fusarium oxysporum f. sp. niveum in grafted watermelon","volume":"22","author":"Xu","year":"2004","journal-title":"J. Shanghai Jiaotong Univ. Agric. Sci."},{"key":"ref40","doi-asserted-by":"publisher","first-page":"49","DOI":"10.1186\/s40168-020-00824-x","article-title":"A predatory myxobacterium controls cucumber fusarium wilt by regulating the soil microbial community","volume":"8","author":"Ye","year":"2020","journal-title":"Microbiome"},{"key":"ref41","doi-asserted-by":"publisher","first-page":"e0123622","DOI":"10.1128\/aem.01236-22","article-title":"Myxobacterial outer membrane \u03b2-1,6-Glucanase induced the cell death of fusarium oxysporum by destroying the Cell Wall integrity","volume":"89","author":"Ye","year":"2023","journal-title":"Appl. Environ. Microbiol."},{"key":"ref42","doi-asserted-by":"publisher","first-page":"1294854","DOI":"10.3389\/fmicb.2023.1294854","article-title":"Active substances of myxobacteria against plant diseases and their action mechanisms","volume":"14","author":"Zhang","year":"2024","journal-title":"Front. Microbiol."},{"key":"ref43","doi-asserted-by":"publisher","first-page":"1089","DOI":"10.1038\/s41396-023-01423-y","article-title":"Predation of oomycetes by myxobacteria via a specialized CAZyme system arising from adaptive evolution","volume":"17","author":"Zhang","year":"2023","journal-title":"ISME J."},{"key":"ref44","doi-asserted-by":"publisher","first-page":"1076","DOI":"10.1111\/ppa.12355","article-title":"Characterization of the watermelon seedling infection process by fusarium oxysporum f. sp. niveum","volume":"64","author":"Zhang","year":"2015","journal-title":"Plant Pathol."},{"key":"ref45","doi-asserted-by":"publisher","first-page":"128559","DOI":"10.1016\/j.micres.2026.128559","article-title":"Bacterial predator-prey interactions and their potentials in combating plant pathogens","volume":"310","author":"Zhang","year":"2026","journal-title":"Microbiol. Res."},{"key":"ref46","doi-asserted-by":"publisher","first-page":"1421","DOI":"10.3390\/genes12091421","article-title":"Whole-genome sequencing of Corallococcus sp. strain EGB reveals the genetic determinants linking taxonomy and predatory behavior","volume":"12","author":"Zhao","year":"2021","journal-title":"Genes (Basel)"},{"key":"ref47","doi-asserted-by":"publisher","first-page":"92","DOI":"10.1094\/pdis-94-1-0092","article-title":"Race 3, a new and highly virulent race of fusarium oxysporum f. sp. niveum causing fusarium wilt in watermelon","volume":"94","author":"Zhou","year":"2010","journal-title":"Plant Dis."},{"key":"ref48","doi-asserted-by":"publisher","first-page":"107797","DOI":"10.1016\/j.soilbio.2020.107797","article-title":"Prevalent root-derived phenolics drive shifts in microbial community composition and prime decomposition in forest soil","volume":"145","author":"Zwetsloot","year":"2020","journal-title":"Soil Biol. 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