{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T07:01:29Z","timestamp":1790146889452,"version":"4.0.1"},"reference-count":83,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T00:00:00Z","timestamp":1790121600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:p>\n                    Gray mold, caused by the psychrophilic necrotroph\n                    <jats:italic>Botrytis cinerea<\/jats:italic>\n                    , is a leading cause of postharvest losses, and its control is increasingly compromised by the spread of fungicide-resistant field populations and by the failure of conventional biocontrol agents to operate at cold-chain temperatures. Antarctic soils are a rational yet underexploited source of cold-active antagonists. Here we isolated cultivable bacteria from the rhizosphere of\n                    <jats:italic>Deschampsia antarctica<\/jats:italic>\n                    (King George Island) and screened them against\n                    <jats:italic>B. cinerea<\/jats:italic>\n                    at 22\u202f\u00b0C and 0\u202f\u00b0C. Of 57 bacterial isolates, 44 antagonized the pathogen at 22\u202f\u00b0C but only 28 retained activity at 0\u202f\u00b0C. Five isolates with indistinguishable rep-PCR fingerprints of the genus\n                    <jats:italic>Pseudomonas<\/jats:italic>\n                    (\n                    <jats:italic>Pseudomonas fluorescens<\/jats:italic>\n                    group) inhibited mycelial growth by \u226570% at low temperature and were equally effective against fenhexamid-resistant and multidrug-resistant strains, whereas the mesophilic references\n                    <jats:italic>Bacillus subtilis<\/jats:italic>\n                    and\n                    <jats:italic>Bacillus amyloliquefaciens<\/jats:italic>\n                    lost all activity at 0\u202f\u00b0C. Polyphasic and genome-based characterization of the representative strain AN3A02 placed it within the recently proposed genus\n                    <jats:italic>Aquipseudomonas<\/jats:italic>\n                    (GTDB-Tk) and identified it as a putative novel species (ANI 94.89% and dDDH 57.9% against the closest type strain,\n                    <jats:italic>P. Yamanorum<\/jats:italic>\n                    ). Genome mining predicted 10 biosynthetic gene clusters consistent with a multifactorial antagonism combining lipopeptides, high-affinity siderophores and volatile compounds. In contact-free assays, AN3A02 volatiles suppressed mycelial growth and the formation of resistance and reproductive structures at 0\u202f\u00b0C and, on inoculated blueberries and table grapes stored under simulated cold-chain conditions, reduced gray-mold incidence below the level achieved by AN SO\u2082 emitter. These cold-adapted Antarctic\n                    <jats:italic>Pseudomonas<\/jats:italic>\n                    \/\n                    <jats:italic>Aquipseudomonas<\/jats:italic>\n                    strains are promising candidates for the biological control of fungicide-resistant gray mold along the fruit cold chain.\n                  <\/jats:p>","DOI":"10.3389\/fmicb.2026.1969102","type":"journal-article","created":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T06:17:22Z","timestamp":1790144242000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Genomic and phenotypic characterization of Antarctic Pseudomonas\/Aquipseudomonas sp. strains with cold-active antifungal activity against Botrytis cinerea"],"prefix":"10.3389","volume":"17","author":[{"given":"Nicol\u00e1s","family":"Plaza","sequence":"first","affiliation":[{"name":"Instituto de Ciencias Biom\u00e9dicas, Facultad de Ciencias de la Salud, Universidad Aut\u00f3noma de Chile","place":["Santiago, Chile"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mat\u00edas","family":"Poblete-Morales","sequence":"additional","affiliation":[{"name":"Instituto de Ciencias Biom\u00e9dicas, Facultad de Ciencias de la Salud, Universidad Aut\u00f3noma de Chile","place":["Santiago, Chile"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katherine","family":"Garc\u00eda","sequence":"additional","affiliation":[{"name":"Instituto de Ciencias Biom\u00e9dicas, Facultad de Ciencias de la Salud, Universidad Aut\u00f3noma de Chile","place":["Santiago, Chile"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marlen","family":"Barreto","sequence":"additional","affiliation":[{"name":"Instituto de Ciencias Biom\u00e9dicas, Facultad de Ciencias de la Salud, Universidad Aut\u00f3noma de Chile","place":["Santiago, Chile"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sebasti\u00e1n","family":"Lagos-Moraga","sequence":"additional","affiliation":[{"name":"Instituto de Ciencias Biom\u00e9dicas, Facultad de Ciencias de la Salud, Universidad Aut\u00f3noma de Chile","place":["Santiago, Chile"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Evelyn","family":"Silva-Moreno","sequence":"additional","affiliation":[{"name":"Fundaci\u00f3n Arturo L\u00f3pez P\u00e9rez OECI Cancer Center, Centro de Investigaci\u00f3n e Innovaci\u00f3n en C\u00e1ncer","place":["Santiago, Chile"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u00cdtalo M.","family":"Urrutia","sequence":"additional","affiliation":[{"name":"Instituto de Ciencias Biom\u00e9dicas, Facultad de Ciencias de la Salud, Universidad Aut\u00f3noma de Chile","place":["Santiago, Chile"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gino","family":"Corsini","sequence":"additional","affiliation":[{"name":"Instituto de Ciencias Biom\u00e9dicas, Facultad de Ciencias de la Salud, Universidad Aut\u00f3noma de Chile","place":["Santiago, Chile"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,9,23]]},"reference":[{"key":"ref1","volume-title":"Plant Pathology","author":"Agrios","year":"2005"},{"key":"ref2","doi-asserted-by":"publisher","first-page":"871941","DOI":"10.1155\/2013\/871941","article-title":"Molybdate reduction to molybdenum blue by an Antarctic bacterium","volume":"2013","author":"Ahmad","year":"2013","journal-title":"Biomed. 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