{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T16:07:49Z","timestamp":1753891669694,"version":"3.41.2"},"reference-count":42,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2019,8,9]],"date-time":"2019-08-09T00:00:00Z","timestamp":1565308800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Mol. Biosci."],"DOI":"10.3389\/fmolb.2019.00066","type":"journal-article","created":{"date-parts":[[2019,8,9]],"date-time":"2019-08-09T12:03:56Z","timestamp":1565352236000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":4,"title":["A \u201cOne-Stop\u201d Screening Protocol for Haemoglobinopathy Traits and Iron Deficiency in Sri Lanka"],"prefix":"10.3389","volume":"6","author":[{"given":"Angela","family":"Allen","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shiromi","family":"Perera","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luxman","family":"Perera","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rexan","family":"Rodrigo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sachith","family":"Mettananda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Agnes","family":"Matope","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ishari","family":"Silva","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nizri","family":"Hameed","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christopher A.","family":"Fisher","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nancy","family":"Olivieri","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David J.","family":"Weatherall","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephen","family":"Allen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anuja","family":"Premawardhena","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2019,8,9]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"e0188110","DOI":"10.1371\/journal.pone.0188110","article-title":"Iron status and anaemia in Sri Lankan secondary school children: a cross-sectional survey","volume":"12","author":"Allen","year":"2017","journal-title":"PLoS ONE"},{"key":"B2","doi-asserted-by":"publisher","first-page":"72","DOI":"10.1016\/j.bcmd.2019.02.004","article-title":"The p.H63D allele of the HFE gene protects against low iron stores in Sri Lanka","volume":"76","author":"Allen","year":"2019","journal-title":"Blood Cells Mol. Dis"},{"key":"B3","doi-asserted-by":"publisher","first-page":"516","DOI":"10.1111\/ejh.12392","article-title":"Sensitivity and specificity of single-tube osmotic fragility test and its different methods as screening test for thalassemia trait: an alternative to expensive laboratory tests for resource-limited countries","volume":"93","author":"Ansari","year":"2014","journal-title":"Eur. J. Haematol."},{"key":"B4","doi-asserted-by":"publisher","first-page":"3927S","DOI":"10.1093\/jn\/133.11.3927S","article-title":"Micronutrient deficiencies and cognitive function","volume":"133","author":"Black","year":"2003","journal-title":"J. Nutr."},{"key":"B5","doi-asserted-by":"publisher","first-page":"34","DOI":"10.1182\/blood-2003-09-3167","article-title":"Complications of \u03b2 \u2013thalassemia major in North America","volume":"104","author":"Cunningham","year":"2004","journal-title":"Blood"},{"key":"B6","doi-asserted-by":"publisher","first-page":"786","DOI":"10.1016\/S0140-6736(99)08246-X","article-title":"Thalassaemia in Sri Lanka: implications for the future health burden of Asian populations","volume":"355","author":"de Silva","year":"2000","journal-title":"Sri Lanka Thalassaemia Study Group. Lancet"},{"key":"B7","doi-asserted-by":"publisher","first-page":"768","DOI":"10.1126\/science.1224577","article-title":"Hepcidin and the iron-infection axis","volume":"338","author":"Drakesmith","year":"2012","journal-title":"Science"},{"key":"B8","first-page":"364","article-title":"A simplified screening strategy for thalassemia and haemoglobin E in rural communities of Southeast Asia","volume":"82","author":"Fucharoen","year":"2004","journal-title":"Bull. World Health Organ"},{"volume-title":"Prevention of Thalassaemias and Other Haemoglobin Disorders: Volume 1: Principles [Internet], chapter 4, 2nd Ed","year":"2012","author":"Galanelo","key":"B9"},{"key":"B10","doi-asserted-by":"publisher","first-page":"105","DOI":"10.1016\/S0022-3476(96)70196-8","article-title":"Elevated zinc protoporphyrin associated with thalassemia trait and hemoglobin E","volume":"129","author":"Graham","year":"1996","journal-title":"J. Pediatr"},{"key":"B11","first-page":"576","article-title":"False positive rates of thalassaemia screening in rural clinical setting: 10 year experience in Thailand","volume":"40","author":"Jopang","year":"2009","journal-title":"Southeast Asian J. Trop. Med. Public Health"},{"key":"B12","doi-asserted-by":"publisher","first-page":"266","DOI":"10.1136\/jmg.18.4.266","article-title":"Effectiveness of one tube osmotic fragility screening in detecting \u03b2- thalassaemia trait","volume":"18","author":"Kattamis","year":"1981","journal-title":"J. Med. Genet."},{"key":"B13","doi-asserted-by":"publisher","first-page":"e29968","DOI":"10.1371\/journal.pone.0029968","article-title":"Iron availability increases the pathogenic potential of Salmonella typhimurium and other enteric pathogens at the intestinal epithelial interface","volume":"7","author":"Kortman","year":"2012","journal-title":"PLoS ONE"},{"key":"B14","first-page":"1","article-title":"DichloroIndophenol (DCIP) precipitation test: a new screening test for HbE and H","volume":"15","author":"Kulapongs","year":"1976","journal-title":"J Paediatr Soc Thai"},{"key":"B15","doi-asserted-by":"crossref","first-page":"2167","DOI":"10.1093\/clinchem\/38.11.2167","article-title":"Clinical utility of zinc protoporphyrin","volume":"38","author":"Labb\u00e9","year":"1992","journal-title":"Clin. Chem."},{"key":"B16","doi-asserted-by":"publisher","first-page":"480","DOI":"10.2471\/BLT.06.036673","article-title":"Global epidemiology of haemoglobin disorders and derived service indicators","volume":"86","author":"Modell","year":"2008","journal-title":"Bull World Health Organiz"},{"key":"B17","doi-asserted-by":"publisher","first-page":"1113","DOI":"10.1136\/bmj.2.6145.1113","article-title":"The adverse effects of iron repletion on the course of certain infections","volume":"2","author":"Murray","year":"1978","journal-title":"Br. Med. J"},{"volume-title":"Prevention of Thalassaemias and Other Haemoglobin Disorders: Volume 2: Laboratory Protocols [Internet], chapter 2, 2nd Ed","year":"2012","author":"Old","key":"B18"},{"key":"B19","doi-asserted-by":"publisher","first-page":"309","DOI":"10.1016\/j.hoc.2015.11.003","article-title":"Iron deficiency anaemia: Problems in diagnosis and prevention at the population level","volume":"30","author":"Parischa","year":"2016","journal-title":"Hematol. Oncol. Clin. N. Am"},{"key":"B20","doi-asserted-by":"publisher","first-page":"636","DOI":"10.1172\/JCI101847","article-title":"The osmotic resistance (fragility) of human red cells","volume":"26","author":"Parpart","year":"1947","journal-title":"J. Clin. Invest"},{"key":"B21","doi-asserted-by":"publisher","first-page":"117","DOI":"10.1111\/bjh.13241","article-title":"Epistasis and the sensitivity of phenotypic screens for beta thalassaemia","volume":"169","author":"Penman","year":"2015","journal-title":"Br. J. Haematol."},{"key":"B22","doi-asserted-by":"publisher","first-page":"475","DOI":"10.1111\/bjh.14437","article-title":"The evolutionary and clinical implications of the uneven distribution of the frequency of the inherited haemoglobin variants over short geographical distances","volume":"176","author":"Premawardhena","year":"2017","journal-title":"Br. J. Haematol"},{"key":"B23","doi-asserted-by":"publisher","first-page":"387","DOI":"10.11613\/BM.2017.040","article-title":"Evaluation of staff performance and interpretation of the screening program for prevention of thalassemia","volume":"27","author":"Prommetta","year":"2017","journal-title":"Biochem. Med."},{"key":"B24","doi-asserted-by":"publisher","first-page":"11","DOI":"10.1016\/j.bcmd.2018.01.003","article-title":"Haemoglobin variants, iron status and anaemia in Sri Lankan adolescents with low red cell indices: a cross sectional survey","volume":"71","author":"Rodrigo","year":"2018","journal-title":"Blood Cells Mol. Dis."},{"key":"B25","doi-asserted-by":"publisher","first-page":"113","DOI":"10.1309\/FUF9EVGQ24V1PKTP","article-title":"A reliable screening protocol for thalassemia and hemoglobinopathies in pregnancy: an alternative approach to electronic blood cell counting","volume":"123","author":"Sanchaisuriya","year":"2005","journal-title":"Am. J. Clin. Pathol."},{"key":"B26","first-page":"1538","article-title":"Validation of osmotic fragility test and Dichlorophenol Indophenol precipitation test for screening of thalassaemia and HbE","volume":"36","author":"Sangkitporn","year":"2005","journal-title":"Southeast Asian J. Trop. Med. Public Health"},{"key":"B27","doi-asserted-by":"publisher","first-page":"647","DOI":"10.1007\/s00277-008-0490-z","article-title":"Thalassemia and hemoglobinopathies in pregnant Lao women: carrier screening, prevalence and molecular basis","volume":"87","author":"Savongsy","year":"2008","journal-title":"Ann. Hematol"},{"key":"B28","doi-asserted-by":"publisher","first-page":"133","DOI":"10.1016\/S0140-6736(06)67962-2","article-title":"Effects of routine prophylactic supplementation with iron and folic acid on admission to hospital and mortality in preschool children in a high malaria transmission setting: community-based, randomised, placebo-controlled trial","volume":"367","author":"Sazawal","year":"2006","journal-title":"Lancet"},{"key":"B29","doi-asserted-by":"publisher","first-page":"60","DOI":"10.4038\/cmj.v49i2.3264","article-title":"Blood lead levels of children before and after introduction of unleaded petrol","volume":"49","author":"Senanayake","year":"2004","journal-title":"Ceylon. Med J"},{"key":"B30","doi-asserted-by":"publisher","first-page":"942","DOI":"10.1046\/j.1365-141.2003.04704.x","article-title":"Determination of the breakpoint and molecular diagnosis of a common a-thalassaemia-1 deletion in the Indian population","volume":"123","author":"Shaji","year":"2003","journal-title":"Br. J. Haematol"},{"key":"B31","doi-asserted-by":"publisher","first-page":"213","DOI":"10.1111\/ijlh.13035","article-title":"Erythrocyte indices in a large cohort of \u03b2-thalassaemia carrier: implication for population screening in an area with high prevalence and heterogeneity of thalassaemia","volume":"41","author":"Singha","year":"2019","journal-title":"Int. J. Lab. Hematol."},{"key":"B32","doi-asserted-by":"publisher","first-page":"250","DOI":"10.1182\/blood.V98.1.250","article-title":"A rapid and reliable 7-deletion multiplex polymerase chain reaction assay for \u03b1-thalassemia","volume":"98","author":"Tan","year":"2001","journal-title":"Blood"},{"key":"B33","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1182\/blood.V49.3.455.455","article-title":"Free erythrocyte porphyrin:hemoglobin ratios, serum ferritin, and transferrin saturation levels during treatment of infants with iron-deficiency anemia","volume":"49","author":"Thomas","year":"1977","journal-title":"Blood"},{"key":"B34","doi-asserted-by":"publisher","first-page":"205","DOI":"10.1136\/jcp.47.3.205","article-title":"Zinc protoporphyrin assays in patients with alpha and beta thalassaemia trait","volume":"47","author":"Tillyer","year":"1994","journal-title":"J. Clin. Pathol."},{"key":"B35","doi-asserted-by":"publisher","first-page":"193","DOI":"10.1016\/j.hoc.2017.11.006","article-title":"Clinical classification, screening, and diagnosis for thalassemia","volume":"32","author":"Viprakasit","year":"2018","journal-title":"Hematol. Oncol. Clin. N. Am"},{"key":"B36","doi-asserted-by":"crossref","DOI":"10.1002\/9780470696705","volume-title":"The Thalassaemia Syndromes, 4th Ed","author":"Weatherall","year":"2001"},{"key":"B37","doi-asserted-by":"publisher","first-page":"4331","DOI":"10.1182\/blood-2010-01-251348","article-title":"The inherited diseases of hemoglobin are an emerging global health burden","volume":"115","author":"Weatherall","year":"2010","journal-title":"Blood"},{"key":"B38","unstructured":"Assessing the iron status of populations: including literature reviews: report of a Joint World Health Organization\/ Centers for Disease Control and Prevention Technical Consultation on the Assessment of Iron Status at the Population Level, 2004 \u2013 2nd ed, Geneva: World Health Organization2007"},{"volume-title":"A Guide for Programme Managers","year":"2001","key":"B39"},{"key":"B40","first-page":"145","article-title":"Screening for the carriers of thalassemias and abnormal hemoglobins at the community level","volume":"33","author":"Winichagoon","year":"2002","journal-title":"Southeast Asian J. Trop. Med. Public Health"},{"volume-title":"World Medical Association General Assembly. Declaration of Helsinki. Ethical Principles for Medical Research Involving Human Subjects.","year":"2008","key":"B41"},{"key":"B42","doi-asserted-by":"publisher","first-page":"511","DOI":"10.1016\/S0140-6736(07)61235-5","article-title":"Nutritional iron deficiency","volume":"370","author":"Zimmerman","year":"2007","journal-title":"Lancet"}],"container-title":["Frontiers in Molecular Biosciences"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/article\/10.3389\/fmolb.2019.00066\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,1,16]],"date-time":"2021-01-16T11:15:08Z","timestamp":1610795708000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/article\/10.3389\/fmolb.2019.00066\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,8,9]]},"references-count":42,"alternative-id":["10.3389\/fmolb.2019.00066"],"URL":"https:\/\/doi.org\/10.3389\/fmolb.2019.00066","relation":{},"ISSN":["2296-889X"],"issn-type":[{"type":"electronic","value":"2296-889X"}],"subject":[],"published":{"date-parts":[[2019,8,9]]},"article-number":"66"}}