{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T17:02:57Z","timestamp":1790614977944,"version":"4.1.0"},"reference-count":141,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2021,1,13]],"date-time":"2021-01-13T00:00:00Z","timestamp":1610496000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100007465","name":"Universitetet i Troms\u00f8","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007465","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Mol. Biosci."],"abstract":"<jats:p>\n                    Bacterial extracellular vesicles (EVs) have a vital role in bacterial pathogenesis. However, to date, the small RNA-cargo of EVs released by the opportunistic pathogen\n                    <jats:italic>Staphylococcus aureus<\/jats:italic>\n                    has not been characterized. Here, we shed light on the association of small RNAs with EVs secreted by\n                    <jats:italic>S. aureus<\/jats:italic>\n                    MSSA476 cultured in iron-depleted bacteriologic media supplemented with a subinhibitory dosage of vancomycin to mimic infection condition. Confocal microscopy analysis on intact RNase-treated EVs indicated that RNA is associated with EV particles. Transcriptomic followed by bioinformatics analysis of EV-associated RNA revealed the presence of potential gene regulatory small RNAs and high levels of tRNAs. Among the EV-associated enriched small RNAs were SsrA, RsaC and RNAIII. Our finding invites new insights into the potential role of EV-associated RNA as a modulator of host-pathogen interaction.\n                  <\/jats:p>","DOI":"10.3389\/fmolb.2020.566207","type":"journal-article","created":{"date-parts":[[2021,1,14]],"date-time":"2021-01-14T03:17:18Z","timestamp":1610594238000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":42,"title":["Transcriptome Profiling of Staphylococcus aureus Associated Extracellular Vesicles Reveals Presence of Small RNA-Cargo"],"prefix":"10.3389","volume":"7","author":[{"given":"Bishnu","family":"Joshi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bhupender","family":"Singh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aftab","family":"Nadeem","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fatemeh","family":"Askarian","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sun Nyunt","family":"Wai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mona","family":"Johannessen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kristin","family":"Hegstad","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2021,1,13]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"e0210082","DOI":"10.1371\/journal.pone.0210082","article-title":"Analysis of colony phase variation switch in Acinetobacter baumannii clinical isolates","volume":"14","author":"Ahmad","year":"2019","journal-title":"PLoS ONE"},{"key":"B2","doi-asserted-by":"publisher","first-page":"e00176","DOI":"10.1128\/mSphere.00176-19","article-title":"Extracellular vesicle-mediated RNA release in Histoplasma capsulatum","volume":"4","author":"Alves","year":"2019","journal-title":"mSphere"},{"key":"B3","doi-asserted-by":"publisher","first-page":"e01439","DOI":"10.1128\/AAC.01439-18","article-title":"Antibiotics stimulate formation of vesicles in Staphylococcus aureus in both phage-dependent and-independent fashions and via different routes","volume":"63","author":"Andreoni","year":"2019","journal-title":"Antimicrob. 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