{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,27]],"date-time":"2026-05-27T12:46:44Z","timestamp":1779886004219,"version":"3.53.1"},"reference-count":36,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2021,5,28]],"date-time":"2021-05-28T00:00:00Z","timestamp":1622160000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001407","name":"Department of Biotechnology, Ministry of Science and Technology, India","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001407","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Mol. Biosci."],"abstract":"<jats:p><jats:italic>Plasmodium falciparum<\/jats:italic> is a causative agent for malaria and has a complex life cycle in human and mosquito hosts. During its life cycle, the malarial parasite <jats:italic>Plasmodium<\/jats:italic> goes through different asexual and sexual stages, in humans and mosquitoes. Expression of stage-specific proteins is important for successful completion of its life cycle and requires tight gene regulation. In the case of <jats:italic>Plasmodium<\/jats:italic>, due to relative paucity of the transcription factors, it is postulated that posttranscriptional regulation plays an important role in stage-specific gene expression. Translation repression of specific set of mRNA has been reported in gametocyte stages of the parasite. A conserved element present in the 3\u2032UTR of some of these transcripts was identified. Phosphatidylinositol 5-phosphate 4-kinase (PIP4K2A) was identified as the protein that associates with these RNA. We now show that the RNA binding activity of PIP4K2A is independent of its kinase activity. We also observe that PIP4K2A is imported into the parasite from the host on <jats:italic>Plasmodium berghei<\/jats:italic> and <jats:italic>Toxoplasma gondii.<\/jats:italic> The RNA binding activity of PIP4K2A seems to be conserved across species from <jats:italic>Drosophila<\/jats:italic> and <jats:italic>C. elegans<\/jats:italic> to humans, suggesting that the RNA binding activity of PIP4K may be important, and there may be host transcripts that may be regulated by PIP4K2A. These results identify a novel RNA binding role for PIP4K2A that may not only play a role in <jats:italic>Plasmodium<\/jats:italic> propagation but may also function in regulating gene expression in multicellular organisms.<\/jats:p>","DOI":"10.3389\/fmolb.2021.631281","type":"journal-article","created":{"date-parts":[[2021,5,28]],"date-time":"2021-05-28T13:10:58Z","timestamp":1622207458000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":7,"title":["Conserved RNA Binding Activity of Phosphatidyl Inositol 5-Phosphate 4-Kinase (PIP4K2A)"],"prefix":"10.3389","volume":"8","author":[{"given":"Jatin","family":"Behari","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pranita","family":"Borkar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arya","family":"Vindu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vishal","family":"Dandewad","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sindhuri","family":"Upadrasta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dhanasekaran","family":"Shanmugam","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vasudevan","family":"Seshadri","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2021,5,28]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"e29123","DOI":"10.7554\/eLife.29123","article-title":"Inhibition of PIP4K\u03b3 Ameliorates the Pathological Effects of Mutant Huntingtin Protein","volume":"6","author":"Al-Ramahi","year":"2017","journal-title":"Elife"},{"key":"B2","doi-asserted-by":"publisher","first-page":"223","DOI":"10.1042\/BJ20100341","article-title":"PIP4K\u03b2 Interacts with and Modulates Nuclear Localization of the High-Activity PtdIns5P-4-kinase Isoform PIP4K\u03b1","volume":"430","author":"Bultsma","year":"2010","journal-title":"Biochem. 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