{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T22:30:20Z","timestamp":1792017020142,"version":"4.4.0"},"reference-count":39,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,1,19]],"date-time":"2022-01-19T00:00:00Z","timestamp":1642550400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Mol. Biosci."],"abstract":"<jats:p><jats:bold>Background:<\/jats:bold> Beh\u00e7et\u2019s disease (BD) is a chronic autoimmune disease. The early diagnosis of BD is very important to avoid serious and\/or fatal complications such as eye damage, severe neurological involvement, and large vessel occlusion. New, sensitive biomarkers would aid in rapid diagnosis, the monitoring of disease activity, and the response to treatment.<\/jats:p><jats:p><jats:bold>Methods:<\/jats:bold> This study\u2019s aim is to identify two immune system-related BD biomarkers. We measured long non-coding RNAs (lncRNAs) <jats:italic>NEAT1<\/jats:italic> (nuclear-enriched abundant transcript 1), and <jats:italic>lnc-DC<\/jats:italic> (lncRNA in dendritic cells) in serum by real-time polymerase chain reaction (RT-PCR) in 52 BD patients and 52 controls. We analyzed the association between <jats:italic>NEAT1<\/jats:italic> and <jats:italic>lnc-DC<\/jats:italic> and the clinical parameters of BD. Receiver operating characteristic (ROC) curve analysis was performed to explore the diagnostic performance of the studied genes.<\/jats:p><jats:p><jats:bold>Results:<\/jats:bold> Compared to controls, the significant upregulation of <jats:italic>NEAT1<\/jats:italic> {median [interquartile range (IQR)] = 1.68 (0.38\u20137.7), <jats:italic>p &amp;lt;<\/jats:italic> 0.0001} and downregulation of <jats:italic>lnc-DC<\/jats:italic> [median (IQR) = 0.2 (0.12\u20131.39), <jats:italic>p =<\/jats:italic> 0.03] were detected in the sera collected from BD patients. Higher serum expression levels of <jats:italic>NEAT1<\/jats:italic> and <jats:italic>lnc-DC<\/jats:italic> were significantly associated with the following clinical presentations: cutaneous lesions, vascular manifestations, articular manifestations, neurological manifestations, and higher disease activity score. Also, high <jats:italic>NEAT1<\/jats:italic> levels were significantly associated with a negative pathergy test, while higher <jats:italic>lnc-DC<\/jats:italic> was significantly associated with a positive family history. ROC curves showed that <jats:italic>NEAT1<\/jats:italic> and <jats:italic>lnc-DC<\/jats:italic> levels in serum could be used as predictors of BD with high specificity and fair sensitivity. <jats:italic>NEAT1<\/jats:italic> had an area under the curve (AUC) of 0.692 (95% CI: 0.591\u20130.794, <jats:italic>p<\/jats:italic> = 0.001), and <jats:italic>lnc-DC<\/jats:italic> had an AUC of 0.615 (95% CI: 0.508\u20130.723, <jats:italic>p<\/jats:italic> = 0.043).<\/jats:p><jats:p><jats:bold>Conclusion:<\/jats:bold> Serum lncRNAs <jats:italic>NEAT1<\/jats:italic> and <jats:italic>lnc-DC<\/jats:italic> are biomarkers for BD.<\/jats:p>","DOI":"10.3389\/fmolb.2021.797689","type":"journal-article","created":{"date-parts":[[2022,1,19]],"date-time":"2022-01-19T17:57:59Z","timestamp":1642615079000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":13,"title":["Expression of lncRNAs NEAT1 and lnc-DC in Serum From Patients With Beh\u00e7et\u2019s Disease Can Be Used as Predictors of Disease"],"prefix":"10.3389","volume":"8","author":[{"given":"Shereen Rashad","family":"Mohammed","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Omayma O.","family":"Abdelaleem","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fatma A.","family":"Ahmed","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmed Ali","family":"Abdelaziz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hoda Abdelbadie","family":"Hussein","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hanaa M.","family":"Eid","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marwa","family":"Kamal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mostafa Ahmed","family":"Ezzat","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marwa A.","family":"Ali","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2022,1,19]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"861","DOI":"10.1038\/nm.4135","article-title":"P53 Induces Formation of NEAT1 LncRNA-Containing Paraspeckles that Modulate Replication Stress Response and Chemosensitivity","volume":"22","author":"Adriaens","year":"2016","journal-title":"Nat. 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