{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,27]],"date-time":"2026-05-27T13:09:45Z","timestamp":1779887385693,"version":"3.53.1"},"reference-count":50,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2025,4,16]],"date-time":"2025-04-16T00:00:00Z","timestamp":1744761600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100021171","name":"Basic and Applied Basic Research Foundation of Guangdong Province","doi-asserted-by":"publisher","award":["2023 \u5e74 15150 \u6708 12476 \u65e5"],"award-info":[{"award-number":["2023 \u5e74 15150 \u6708 12476 \u65e5"]}],"id":[{"id":"10.13039\/501100021171","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82471914 82171877"],"award-info":[{"award-number":["82471914 82171877"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Mol. Biosci."],"abstract":"<jats:sec><jats:title>Introduction<\/jats:title><jats:p>The identification of fatal hypothermia remains a significant challenge in forensic medicine. Metabolomics, which reflects the overall changes in endogenous metabolites within an organism, holds substantial value in the exploration of disease mechanisms and the screening of molecular markers.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Using ultra-high-performance liquid chromatography\u2012mass spectrometry (UHPLC\u2012MS), we conducted a metabolomic analysis of serum, heart, lung, and kidney tissues from mice with fatal hypothermia.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>A total of 67 metabolites significantly differed across all the tissues, involving pathways such as the TCA cycle, fatty acid oxidation, arginine metabolism, histamine metabolism, and antioxidant-related pathways. Each tissue also displayed unique metabolic alterations. Additionally, we observed significant differences in the metabolomic profiles of kidney tissues from mice with different survival times.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Our findings contribute to elucidate the underlying mechanisms involved and provide a foundation for the forensic identification of markers of fatal hypothermia.<\/jats:p><\/jats:sec>","DOI":"10.3389\/fmolb.2025.1563642","type":"journal-article","created":{"date-parts":[[2025,4,16]],"date-time":"2025-04-16T04:10:44Z","timestamp":1744776644000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":2,"title":["Metabolomic analysis of fatal hypothermia using ultra-high-performance liquid chromatography\u2012mass spectrometry"],"prefix":"10.3389","volume":"12","author":[{"given":"Jia-Hao","family":"Li","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jia-Li","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian-Wen","family":"Song","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei-Liang","family":"Deng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin-Zhi","family":"Cao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhong-Wen","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ding-Hao","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Song","family":"Yu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qi","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2025,4,16]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"13987","DOI":"10.1038\/s41598-018-31055-8","article-title":"Development of a novel UHPLC-MS\/MS-based platform to quantify amines, amino acids and methylarginines for applications in human disease phenotyping","volume":"8","author":"Ahmetaj-Shala","year":"2018","journal-title":"Sci. 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