{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,5]],"date-time":"2026-11-05T01:19:58Z","timestamp":1793841598073,"version":"4.6.1"},"reference-count":24,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2025,10,2]],"date-time":"2025-10-02T00:00:00Z","timestamp":1759363200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Mol. Biosci."],"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p>This study aims to unveil the interleukin-17 (IL-17) and interferon-gamma (IFN-\u03b3) levels in the peripheral blood of patients with Hashimoto\u2019s thyroiditis (HT) and their association with thyroid function.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We selected 68 HT patients admitted to our hospital and 36 healthy individuals undergoing physical examinations as controls. Clinical data were collected, and serum IL-17 and IFN-\u03b3 levels were measured. The Pearson method was used to analyze the correlations between IL-17 and IFN-\u03b3 levels and thyroid function parameters. A logistic regression analysis model was employed to evaluate the influencing factors of HT. ROC curves were utilized to assess the diagnostic value of IL-17 and IFN-\u03b3 for the occurrence of HT.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Serum IL-17 and IFN-\u03b3 levels were higher in both HT patients with hypothyroidism and those without hypothyroidism compared to healthy controls (<jats:italic>P<\/jats:italic> &amp;lt; 0.05). IL-17 levels demonstrated a positive correlation with TGAb, TPOAb, and TSH among HT patients, while showing a negative correlation with FT4 (<jats:italic>P<\/jats:italic> &amp;lt; 0.05). Similarly, IFN-\u03b3 levels were positively related to TGAb and TPOAb (<jats:italic>P<\/jats:italic> &amp;lt; 0.05). IL-17 and IFN-\u03b3 levels were identified as influencing factors for the occurrence of HT (OR: 1.012, 1.028; <jats:italic>P<\/jats:italic> &amp;lt; 0.05). The optimal cutoff values for distinguishing HT from healthy controls were &amp;gt;629.77 pg\/mL for IL-17, &amp;gt;286.04 ng\/L for IFN-\u03b3, and (&amp;gt;683.02 pg\/mL, &amp;gt;252.73 ng\/L) for the combination of both. The areas under the curves were 0.854 (0.771\u20130.936), 0.795 (0.697\u20130.894), and 0.903 (0.846\u20130.960), respectively.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Serum IL-17 and IFN-\u03b3 levels are highly expressed in HT patients, and both are closely related to thyroid function and autoantibody levels. They possessed a certain value for the early diagnosis of HT.<\/jats:p><\/jats:sec>","DOI":"10.3389\/fmolb.2025.1645736","type":"journal-article","created":{"date-parts":[[2025,10,2]],"date-time":"2025-10-02T04:20:41Z","timestamp":1759378841000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":6,"title":["Expression levels of interleukin-17 and interferon-\u03b3 in peripheral blood and relationship with thyroid function in patients with Hashimoto\u2019s thyroiditis"],"prefix":"10.3389","volume":"12","author":[{"given":"Zhiyong","family":"Huang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiaming","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Si","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingfeng","family":"Mao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Boyong","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2025,10,2]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"204","DOI":"10.1186\/s13075-024-03437-z","article-title":"Semaphorin 5A promotes Th17 differentiation via PI3K-Akt-mTOR in systemic lupus erythematosus","volume":"26","author":"Chen","year":"2024","journal-title":"Arthritis Res. 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