{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,24]],"date-time":"2026-02-24T08:43:32Z","timestamp":1771922612539,"version":"3.50.1"},"reference-count":50,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,1,20]],"date-time":"2026-01-20T00:00:00Z","timestamp":1768867200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Mol. Biosci."],"abstract":"<jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>Colorectal cancer (CRC) is the third most diagnosed cancer worldwide and the second leading cause of cancer-related deaths. A major challenge in CRC treatment is drug resistance, which limits the efficacy of conventional therapies. Ferroptosis, an iron-dependent form of regulated cell death driven by the accumulation of reactive oxygen species (ROS), has emerged as a promising therapeutic strategy. Erastin (ER), a small-molecule compound, induces ferroptosis through ROS accumulation.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We performed microarray gene expression analysis on two CRC cell lines, HCT116 and HT-29, to examine the transcriptional response to ER exposure and identify differentially expressed genes and pathways involved in ER-induced ferroptosis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Our gene expression analysis revealed distinct transcriptional profiles between the two cell lines, and 26 transcripts commonly enriched in response to ER treatment were identified in both HCT116 and HT-29 cells. Notably, several of these genes\u2014including\n                      <jats:italic>ASNS, PCK2, CHAC1, and DDIT4<\/jats:italic>\n                      \u2014were significantly enriched, suggesting a conserved ferroptotic response. The induction of these genes was further confirmed in an additional CRC cell line, DLD-1. Interestingly,\n                      <jats:italic>SOD1<\/jats:italic>\n                      and\n                      <jats:italic>NQO1<\/jats:italic>\n                      genes, involved in oxidative stress response, were significantly upregulated by ER in HCT116 cells.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      Our findings highlight\n                      <jats:italic>ASNS, CHAC1, PCK2, DDIT4, and ATF3\/4<\/jats:italic>\n                      as potential biomarkers for ferroptosis in CRC. Monitoring the expression of these genes may help identify patients who are responsive to ferroptosis inducers and facilitate the development of personalized treatment strategies.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fmolb.2025.1680206","type":"journal-article","created":{"date-parts":[[2026,1,20]],"date-time":"2026-01-20T09:00:23Z","timestamp":1768899623000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Gene expression profiling identifies ferroptosis-related genes and pathways in human colon cancers cell lines"],"prefix":"10.3389","volume":"12","author":[{"given":"M.","family":"Balik-Meisner","sequence":"first","affiliation":[{"name":"Sciome LLC","place":["Durham, NC, United States"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D.","family":"Phadke","sequence":"additional","affiliation":[{"name":"Sciome LLC","place":["Durham, NC, United States"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D.","family":"Mav","sequence":"additional","affiliation":[{"name":"Sciome LLC","place":["Durham, NC, United States"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"Shah","sequence":"additional","affiliation":[{"name":"Sciome LLC","place":["Durham, NC, United States"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K. 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