{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T13:23:07Z","timestamp":1785936187947,"version":"3.56.0"},"reference-count":42,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,1,23]],"date-time":"2026-01-23T00:00:00Z","timestamp":1769126400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004242","name":"Princess Nourah Bint Abdulrahman University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004242","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Mol. Biosci."],"abstract":"\n Background and aim<\/jats:title>\n \n Chronic kidney disease (CKD) incorporates a variety of progressive kidney function declines, ranging from mild impairment to end-stage renal disease (ESRD). As a global public health problem, early detection and monitoring remain critical for improving patient outcomes. This study aimed to evaluate the potential diagnostic significance and interrelationships of serum sclerostin,\n lncRNA H19<\/jats:italic>\n ,\n lncRNA MEG3<\/jats:italic>\n ,\n lncRNA MIAT<\/jats:italic>\n ,\n miR-135a<\/jats:italic>\n , and\n miR-29a<\/jats:italic>\n in patients with CKD and those undergoing hemodialysis.\n <\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n \n A total of 150 participants were enrolled in this present case\u2013control study: 50 hemodialysis patients, 50 CKD patients, and 50 healthy controls. Circulating serum sclerostin levels were measured using ELISA. Real-time quantitative PCR (RT-qPCR) was used to measure the circulating levels of lncRNAs (\n H19<\/jats:italic>\n ,\n MEG3<\/jats:italic>\n , and\n MIAT<\/jats:italic>\n ) and microRNAs (\n miR-135a<\/jats:italic>\n and\n miR-29a<\/jats:italic>\n ). A spectrophotometer was used to determine the levels of calcium, creatinine, urea, and phosphorus in the blood.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n Both the CKD and hemodialysis groups displayed significantly elevated levels of all studied biomarkers compared with controls. However, the highest levels of sclerostin, lncRNAs (\n H19<\/jats:italic>\n ,\n MEG3<\/jats:italic>\n , and\n MIAT<\/jats:italic>\n ), and microRNAs (\n miR-135a<\/jats:italic>\n and\n miR-29a<\/jats:italic>\n ) were found in the hemodialysis group. LncRNA\n MIAT<\/jats:italic>\n ,\n miR-135a<\/jats:italic>\n , and\n miR-29a<\/jats:italic>\n showed high sensitivity and specificity in distinguishing CKD patients from healthy controls. Additionally,\n H19<\/jats:italic>\n ,\n MEG3<\/jats:italic>\n , and\n miR-29a<\/jats:italic>\n displayed strong differentiating ability between CKD and hemodialysis patients. All studied biomarkers exhibited strong positive correlations. Predictive modeling identified\n lncRNAs H19<\/jats:italic>\n ,\n MEG3<\/jats:italic>\n , and\n MIAT<\/jats:italic>\n as significant predictors of hemodialysis status, while sclerostin,\n MEG3<\/jats:italic>\n , and\n MIAT<\/jats:italic>\n were the strongest predictors of CKD.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion<\/jats:title>\n \n Serum sclerostin, lncRNAs (\n H19<\/jats:italic>\n ,\n MEG3<\/jats:italic>\n , and\n MIAT<\/jats:italic>\n ), and microRNAs (\n miR-135a<\/jats:italic>\n and\n miR-29a<\/jats:italic>\n ) are significantly interrelated and may serve as promising non-invasive molecular biomarkers for early detection and monitoring of CKD and hemodialysis patients.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.3389\/fmolb.2025.1703108","type":"journal-article","created":{"date-parts":[[2026,1,23]],"date-time":"2026-01-23T06:48:43Z","timestamp":1769150923000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":1,"title":["Investigating circulating expression profile for H19, MEG3, and MIAT long noncoding RNAs with miR-135a and miR-29a in chronic kidney disease and renal hemodialysis patients: interrelations with serum sclerostin"],"prefix":"10.3389","volume":"12","author":[{"given":"Marwa A.","family":"Dahpy","sequence":"first","affiliation":[{"name":"Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Assiut University","place":["Assiut, Egypt"]},{"name":"Department of Medical Biochemistry and Molecular Biology, Armed Forces College of Medicine (AFCM)","place":["Cairo, Egypt"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marwa K.","family":"Khairallah","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Faculty of Medicine, Assiut University","place":["Assiut, Egypt"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rania","family":"Naguib","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, College of Medicine, Princess Nourah bint Abdulrahman University","place":["Riyadh, Saudi Arabia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mona A.","family":"Khalil","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, Faculty of Medicine, Al-Azhar, University","place":["Cairo, Egypt"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Salwa","family":"Seif Eldin","sequence":"additional","affiliation":[{"name":"Department of Basic Medical Sciences, College of Medicine, Princess Nourah Bint Abdulrahman University","place":["Riyadh, Saudi Arabia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marwa A.","family":"Sabet","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Faculty of Pharmacy, Sphinx University","place":["New-Assiut, Egypt"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amira A.","family":"Kamel","sequence":"additional","affiliation":[{"name":"Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Assiut University","place":["Assiut, Egypt"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,1,23]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1007\/s00223-024-01261-w","article-title":"The relationship between sclerostin and kidney-related parameters in kidney transplant recipients","author":"Afsar","year":"2024","journal-title":"Calcif. 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