{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,26]],"date-time":"2026-05-26T05:06:10Z","timestamp":1779771970762,"version":"3.53.1"},"reference-count":45,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,5,26]],"date-time":"2026-05-26T00:00:00Z","timestamp":1779753600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Mol. Biosci."],"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Gout is one of the most common inflammatory arthritides. Although identification of monosodium urate crystals in synovial fluid is the diagnostic gold standard, the invasive procedure limits routine use. Non-invasive biomarkers are needed to facilitate timely diagnosis of acute gout flares and to differentiate gout from asymptomatic hyperuricaemia<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Differential expression analysis was performed using the GEO circRNA microarray dataset GSE178825 (peripheral blood mononuclear cells, PBMCs; gout vs. healthy controls). Differentially expressed circRNAs were mapped to their host genes, followed by host-gene-based functional enrichment and protein\u2013protein interaction (PPI) network analyses for candidate prioritization, circMAN1A2, derived from the MAN1A2 locus, was selected for clinical validation. PBMCs were isolated from whole blood samples obtained from 30 patients with acute gout flare, 30 individuals with HUA, and 30 healthy controls. Circularity was confirmed by RNase R resistance, and circMAN1A2 expression was quantified by RT-qPCR. Diagnostic performance was evaluated using receiver operating characteristic (ROC) analysis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Peripheral blood circMAN1A2 levels were significantly lower in patients with gout than in healthy controls, and showed a decreasing trend compared with individuals with HUA. circMAN1A2 showed preliminary diagnostic potential for gout (AUC = 0.86; 95% CI 0.66\u20131.00), with 90.0% sensitivity and 90.1% specificity.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Peripheral blood circMAN1A2 is a promising candidate biomarker for gout diagnosis. Integrating public transcriptomic mining with targeted clinical validation may accelerate biomarker discovery for gout.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fmolb.2026.1791371","type":"journal-article","created":{"date-parts":[[2026,5,26]],"date-time":"2026-05-26T04:50:52Z","timestamp":1779771052000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Peripheral blood circMAN1A2 as a novel diagnostic biomarker for gout: integrated transcriptomic analysis and clinical validation"],"prefix":"10.3389","volume":"13","author":[{"given":"Wenyan","family":"Guo","sequence":"first","affiliation":[{"name":"The Department of Clinical Laboratory, General Hospital of Southern Theater Command","place":["Guangzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kaixi","family":"Liu","sequence":"additional","affiliation":[{"name":"The Department of Clinical Laboratory, Shantou Central Hospital","place":["Shantou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianhong","family":"Sun","sequence":"additional","affiliation":[{"name":"The Department of Clinical Laboratory, Shantou Central Hospital","place":["Shantou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiaoxin","family":"Zhang","sequence":"additional","affiliation":[{"name":"The Department of Clinical Laboratory, The First Affiliated Hospital of Shantou University Medical College","place":["Shantou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoyang","family":"Jiao","sequence":"additional","affiliation":[{"name":"Department of Cell Biology and Genetics, Medical College of Shantou University","place":["Shantou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peiting","family":"Ru","sequence":"additional","affiliation":[{"name":"The Department of Clinical Laboratory, Guangzhou Nansha District Hospital of Traditional Chinese Medicine","place":["Guangzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanrong","family":"Chen","sequence":"additional","affiliation":[{"name":"The Department of Clinical Laboratory, The First Affiliated Hospital of Shantou University Medical College","place":["Shantou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinran","family":"Yang","sequence":"additional","affiliation":[{"name":"The Department of Clinical Laboratory, The First Affiliated Hospital of Shantou University Medical College","place":["Shantou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,5,26]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"425","DOI":"10.1001\/jama.2022.9165","article-title":"Cardiovascular events and gout flares","volume":"328","author":"Anderson","year":"2022","journal-title":"JAMA"},{"key":"B2","doi-asserted-by":"publisher","first-page":"3994","DOI":"10.3390\/cells11243994","article-title":"PICALM and alzheimer's disease: an update and perspectives","volume":"11","author":"Ando","year":"2022","journal-title":"Cells"},{"key":"B3","doi-asserted-by":"publisher","first-page":"123","DOI":"10.1186\/s12916-017-0890-9","article-title":"Impact of comorbidities on gout and hyperuricaemia: an update on prevalence and treatment options","volume":"15","author":"Bardin","year":"2017","journal-title":"BMC Med."},{"key":"B4","doi-asserted-by":"publisher","first-page":"113842","DOI":"10.1016\/j.psychres.2021.113842","article-title":"Bioinformatics analysis of a TF-miRNA-lncRNA regulatory network in major depressive disorder","volume":"299","author":"Bian","year":"2021","journal-title":"Psychiatry Res."},{"key":"B5","doi-asserted-by":"publisher","first-page":"628654","DOI":"10.3389\/fimmu.2021.628654","article-title":"Circ_0088194 promotes the invasion and migration of rheumatoid arthritis fibroblast-like synoviocytes via the miR-766-3p\/MMP2 axis","volume":"12","author":"Cai","year":"2021","journal-title":"Front. 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