{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T11:13:25Z","timestamp":1785582805517,"version":"3.56.0"},"reference-count":47,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,5,18]],"date-time":"2026-05-18T00:00:00Z","timestamp":1779062400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Mol. Biosci."],"abstract":"<jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>\n                      Hepatic ischemia-reperfusion (IR) injury remains a major clinical problem during liver surgery and transplantation, primarily due to oxidative stress, inflammatory activation, and hepatocellular damage. Morroniside (MO), an iridoid glycoside derived from\n                      <jats:italic>Cornus officinalis<\/jats:italic>\n                      , has demonstrated anti-inflammatory, antioxidant, and antifibrotic properties in various experimental models, but its role in hepatic IR injury has not been established. This study aimed to evaluate the effect of a single dose of MO on rat liver subjected to partial ischemia and reperfusion.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Wistar rats were divided into four groups: control (C), ischemia-reperfusion without treatment (CIR), and IR treated with MO at 90 mg\/kg (90MIR) or 270 mg\/kg (270MIR). Hepatic injury was assessed biochemically (ALT, AST, PIIINP, antioxidant capacity, cytokine profile), histologically and immunohistochemically (Collagen III, Survivin, HIF-1\u03b1, Caspase-8, IL-6).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>IR induced significant hepatocellular injury, reflected by elevated aminotransferases, structural disruption, and increased pro-inflammatory cytokines. MO administration produced dose-dependent and heterogeneous effects. At 90 mg\/kg, MO partially attenuated early aminotransferase elevation and reduced TNF-\u03b1 and VEGF levels, suggesting initial hepatoprotective activity; however, hepatocellular damage and fibrosis markers remained elevated after 24 h. At 270 mg\/kg, MO paradoxically decreased antioxidant capacity and did not suppress fibrosis or apoptosis, while only modestly reducing pro-inflammatory cytokines. Histopathological analyses confirmed pronounced liver injury in all IR groups regardless of treatment.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion<\/jats:title>\n                    <jats:p>In conclusion, acute pre-procedural administration of MO did not provide consistent protection against hepatic IR injury, and its effects were dose-dependent and ambiguous. Further studies with prolonged dosing regimens and extended reperfusion times are warranted to clarify MO\u2019s hepatoprotective potential.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fmolb.2026.1798488","type":"journal-article","created":{"date-parts":[[2026,5,17]],"date-time":"2026-05-17T22:10:15Z","timestamp":1779055815000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":1,"title":["Evaluation of the effect of a single dose of morroniside on rat liver subjected to ischemia and reperfusion"],"prefix":"10.3389","volume":"13","author":[{"given":"Ma\u0142gorzata","family":"Trocha","sequence":"first","affiliation":[{"name":"Clinical Department of Diabetology, Faculty of Medicine, Hypertension and Internal Disease, Wroclaw Medical University","place":["Wroclaw, Poland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aleksandra","family":"Kuzan","sequence":"additional","affiliation":[{"name":"Department of Preclinical Sciences, Pharmacology and Medical Diagnostics, Wroclaw University of Science and Technology","place":["Wroclaw, Poland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paulina","family":"Nowotarska","sequence":"additional","affiliation":[{"name":"Department of Biostructure and Animal Physiology, Wroclaw University of Environmental and Life Sciences","place":["Wroclaw, Poland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomasz","family":"Piasecki","sequence":"additional","affiliation":[{"name":"Department of Epizootiology with Exotic Animal and Bird Clinic, Wroclaw University of Environmental and Life Sciences","place":["Wroclaw, Poland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Merwid-L\u0105d","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Faculty of Medicine, Wroclaw Medical University","place":["Wroclaw, Poland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adam","family":"Szel\u0105g","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Faculty of Medicine, Wroclaw Medical University","place":["Wroclaw, Poland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcin","family":"Magdziarz","sequence":"additional","affiliation":[{"name":"Hugo Steinhaus Center, Faculty of Pure and Applied Mathematics, Wroc\u0142aw University of Science and Technology","place":["Wroclaw, Poland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miros\u0142aw","family":"Sopel","sequence":"additional","affiliation":[{"name":"Department of Preclinical Sciences, Pharmacology and Medical Diagnostics, Wroclaw University of Science and Technology","place":["Wroclaw, Poland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alicja Z.","family":"Kucharska","sequence":"additional","affiliation":[{"name":"Department of Fruit, Vegetable and Plant Nutraceutical Technology, Wroc\u0142aw University of Environmental and Life Sciences","place":["Wroclaw, Poland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katarzyna","family":"Madziarska","sequence":"additional","affiliation":[{"name":"Clinical Department of Diabetology, Faculty of Medicine, Hypertension and Internal Disease, Wroclaw Medical University","place":["Wroclaw, Poland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomasz","family":"Soza\u0144ski","sequence":"additional","affiliation":[{"name":"Department of Preclinical Sciences, Pharmacology and Medical Diagnostics, Wroclaw University of Science and Technology","place":["Wroclaw, Poland"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,5,18]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"154199","DOI":"10.1016\/j.phymed.2022.154199","article-title":"Morroniside, a novel GATA3 binding molecule, inhibits hepatic stellate cells activation by enhancing lysosomal acid lipase expression","volume":"103","author":"An","year":"2022","journal-title":"Phytomedicine"},{"key":"B2","doi-asserted-by":"publisher","first-page":"1951","DOI":"10.3748\/WJG.15.1951","article-title":"Histological and biochemical alterations in early-stage lobar ischemia-reperfusion in rat liver","volume":"15","author":"Arab","year":"2009","journal-title":"World J. 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