{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T05:24:36Z","timestamp":1786944276687,"version":"build-2736575974"},"reference-count":26,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T00:00:00Z","timestamp":1786924800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Mol. Biosci."],"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>The goal of this study is to investigate the expression pattern of GANAB in colorectal cancer and the role behind it.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>In order to study the expression level of GANAB in the tissue of colorectal cancer, we used quantitative real-time PCR and immunohistochemistry. In the aspect of biological function, we study it through the experimental models. The molecular mechanism is clarified by RNA pull-down and RNA stability assays. Student's t-tests and one-way ANOVA are used for data analysis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Analysis of tissue microarrays and Colorectal cancer (CRC) cell lines revealed elevated GANAB expression in tumors compared to normal counterparts. Increased GANAB levels were linked to enhanced cell migration, invasion, and proliferation, and correlated with metastatic potential and unfavorable patient outcomes. Functional enrichment studies indicated that GANAB-associated genes participate in the Wnt\/\u03b2-catenin signaling pathway. Immunohistochemistry confirmed positive correlations between GANAB and both c-Myc and \u03b2-catenin. The oncogenic function of GANAB was shown to rely on Wnt pathway activation. Additionally, lncRNA FIRRE, known to be oncogenic in CRC, was found to physically interact with GANAB mRNA\n                      <jats:italic>via<\/jats:italic>\n                      RNA pull-down assays. FIRRE enhances GANAB mRNA stability through direct binding.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Our study demonstrates that GANAB exerts oncogenic effects in CRC by binding directly to lncRNA FIRRE, thereby stabilizing GANAB mRNA and activating the Wnt\/\u03b2-catenin pathway through nuclear accumulation of \u03b2-catenin.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fmolb.2026.1820992","type":"journal-article","created":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T04:34:53Z","timestamp":1786941293000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Glucosidase GANAB drives colorectal cancer progression through Wnt signaling and is modulated by lncRNA FIRRE"],"prefix":"10.3389","volume":"13","author":[{"given":"Yajie","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Gastroenterology, Jinshan Hospital, Fudan University","place":["Shanghai, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenjun","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Anaesthesiology, Shanghai Public Health Clinical Center, Fudan University","place":["Shanghai, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qian","family":"Hua","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, Jinshan Hospital, Fudan University","place":["Shanghai, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhengyang","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, Jinshan Hospital, Fudan University","place":["Shanghai, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miao","family":"Jiang","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, Jinshan Hospital, Fudan University","place":["Shanghai, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,8,17]]},"reference":[{"key":"B8","doi-asserted-by":"publisher","first-page":"96","DOI":"10.7150\/jca.5470","article-title":"Crosstalk between Wnt signaling and RNA processing in colorectal cancer","volume":"4","author":"Bordonaro","year":"2013","journal-title":"J. Cancer"},{"key":"B1","doi-asserted-by":"publisher","first-page":"100086","DOI":"10.1016\/j.insi.2025.100086","article-title":"Unveiling the role of lncRNAs in tumorigenesis: mechanisms, functions, and diagnostic\/therapeutic applications","volume":"1","author":"Cai","year":"2025","journal-title":"In Silico Res. Biomed."},{"key":"B2","doi-asserted-by":"publisher","first-page":"1192","DOI":"10.1016\/j.cell.2012.05.012","article-title":"Wnt\/beta-catenin signaling and disease","volume":"149","author":"Clevers","year":"2012","journal-title":"Cell."},{"key":"B3","doi-asserted-by":"publisher","first-page":"2991","DOI":"10.7150\/ijbs.109593","article-title":"Crosstalk between RNA-binding proteins and non-coding RNAs in tumors: molecular mechanisms, and clinical significance","volume":"21","author":"Cui","year":"2025","journal-title":"Int. J. Biol. Sci."},{"key":"B4","doi-asserted-by":"publisher","first-page":"255","DOI":"10.5603\/FHC.a2020.0026","article-title":"ZNF750 inhibits the proliferation and invasion of melanoma cells through modulating the Wnt\/\u03b2-catenin signaling pathway","volume":"58","author":"Du","year":"2020","journal-title":"Folia Histochem Cytobiol."},{"key":"B5","doi-asserted-by":"publisher","first-page":"198","DOI":"10.1038\/nsmb.2764","article-title":"Topological organization of multichromosomal regions by the long intergenic noncoding RNA firre","volume":"21","author":"Hacisuleyman","year":"2014","journal-title":"Nat. Struct. Mol. Biol."},{"key":"B6","doi-asserted-by":"publisher","first-page":"533","DOI":"10.1038\/nrm4032","article-title":"Specificity and nonspecificity in RNA\u2013protein interactions","volume":"16","author":"Jankowsky","year":"2015","journal-title":"Nat. Rev. Mol. Cell. Biol."},{"key":"B7","doi-asserted-by":"publisher","first-page":"10394","DOI":"10.1038\/s41598-019-46701-y","article-title":"Knockout of glucosidase II beta subunit inhibits growth and metastatic po-tential of lung cancer cells by inhibiting receptor tyrosine kinase activities","volume":"9","author":"Khaodee","year":"2019","journal-title":"Sci. Rep."},{"key":"B9","doi-asserted-by":"publisher","first-page":"817","DOI":"10.1186\/s12885-22-09884-8","article-title":"Glycoprotein \u03b1-Subunit of Glucosidase II (GII\u03b1) is a novel prognostic biomarker correlated with unfavorable outcome of urothelial carcinoma","volume":"22","author":"Lin","year":"","journal-title":"BMC Cancer"},{"key":"B10","doi-asserted-by":"publisher","first-page":"817","DOI":"10.1186\/s12885-022-09884-8","article-title":"Glycoprotein alpha-subunit of Glucosidase II (GIIalpha) is a novel prognostic biomarker correlated with unfavorable outcome of urothelial carcinoma","volume":"22","author":"Lin","year":"","journal-title":"BMC Cancer"},{"key":"B11","doi-asserted-by":"publisher","first-page":"1193","DOI":"10.1016\/j.ajhg.2016.05.004","article-title":"Mutations in GANAB, encoding the glucosidase II\u03b1 subunit, cause autosomal-dominant polycystic kidney and liver disease","volume":"98","author":"Porath","year":"2016","journal-title":"Am. J. Hum. Genet."},{"key":"B12","doi-asserted-by":"publisher","first-page":"306","DOI":"10.1093\/glycob\/cwx006","article-title":"MeCP2 regulated glycogenes contribute to proliferation and apoptosis of gastric cancer cells","volume":"27","author":"Qin","year":"2017","journal-title":"Glycobiology"},{"key":"B13","doi-asserted-by":"publisher","first-page":"917378","DOI":"10.3389\/fonc.2022.917378","article-title":"Corrigendum: upregulation of CCT-3 induces breast cancer cell proliferation through miR-223 competition and Wnt\/\u03b2-Catenin signaling pathway activation","volume":"12","author":"Qu","year":"2022","journal-title":"Front. Oncol."},{"key":"B14","doi-asserted-by":"publisher","first-page":"144892","DOI":"10.1016\/j.gene.2020.144892","article-title":"Role of regulatory miRNAs of the Wnt\/\u03b2-catenin signaling pathway in tumorigenesis of breast cancer","volume":"754","author":"Rahmani","year":"2020","journal-title":"Gene"},{"key":"B15","doi-asserted-by":"publisher","first-page":"e7656","DOI":"10.15252\/msb.20177656","article-title":"Toward an integrated map of genetic interactions in cancer cells","volume":"14","author":"Rauscher","year":"2018","journal-title":"Mol. Syst. Biol."},{"key":"B16","doi-asserted-by":"publisher","first-page":"145","DOI":"10.3322\/caac.21601","article-title":"Colorectal cancer statistics, 2020","volume":"70","author":"Siegel","year":"2020","journal-title":"CA Cancer J. Clin."},{"key":"B17","doi-asserted-by":"publisher","first-page":"96","DOI":"10.1038\/s41580-020-00315-9","article-title":"Gene regulation by long non-coding RNAs and its biological functions","volume":"22","author":"Statello","year":"2021","journal-title":"Nat. Rev. Mol. Cell. Biol."},{"key":"B18","doi-asserted-by":"publisher","first-page":"112609","DOI":"10.1016\/j.celrep.2023.112609","article-title":"High-resolution structural-omics of human liver enzymes","volume":"42","author":"Su","year":"2023","journal-title":"Cell. Rep."},{"key":"B19","doi-asserted-by":"publisher","first-page":"147657","DOI":"10.1016\/j.ijbiomac.2025.147657","article-title":"The interplay between post-transcriptional RNA regulation and Wnt\/\u03b2-Catenin signaling in cancer: a review","volume":"328","author":"Sun","year":"2025","journal-title":"Int. J. Biol. Macromol."},{"key":"B20","doi-asserted-by":"publisher","first-page":"241","DOI":"10.1016\/s0092-8674(02)01014-0","article-title":"The beta-catenin\/TCF-4 complex imposes a crypt progenitor phenotype on colorectal cancer cells","volume":"111","author":"van de Wetering","year":"2002","journal-title":"Cell."},{"key":"B21","doi-asserted-by":"publisher","first-page":"117","DOI":"10.5603\/FHC.a2020.0012","article-title":"Gamma linolenic acid suppresses hypoxia-induced gastric cancer cell growth and epithelial-mesenchymal transition by inhibiting the Wnt\/\u03b2-catenin signaling pathway","volume":"58","author":"Wang","year":"2020","journal-title":"Folia Histochem Cytobiol."},{"key":"B22","doi-asserted-by":"publisher","first-page":"98","DOI":"10.1038\/s41419-022-04509-1","article-title":"LncRNA FIRRE functions as a tumor promoter by interaction with PTBP1 to stabilize BECN1 mRNA and facilitate autophagy","volume":"13","author":"Wang","year":"2022","journal-title":"Cell. Death Dis."},{"key":"B23","doi-asserted-by":"publisher","first-page":"640","DOI":"10.1007\/s11427-018-9461-5","article-title":"Analysis of status and countermeasures of cancer incidence and mortality in China","volume":"62","author":"Wu","year":"2019","journal-title":"Sci. China Life Sci."},{"key":"B24","doi-asserted-by":"publisher","first-page":"124","DOI":"10.1186\/s12943-017-0700-1","article-title":"FOXP3 promotes tumor growth and metastasis by activating Wnt\/beta-catenin signaling pathway and EMT in non-small cell lung cancer","volume":"16","author":"Yang","year":"2017","journal-title":"Mol. Cancer"},{"key":"B25","doi-asserted-by":"publisher","first-page":"666","DOI":"10.1016\/j.ccr.2014.03.010","article-title":"A long noncoding RNA activated by TGF-beta promotes the invasion-metastasis cascade in hepatocellular carcinoma","volume":"25","author":"Yuan","year":"2014","journal-title":"Cancer Cell."},{"key":"B26","doi-asserted-by":"publisher","first-page":"225","DOI":"10.1186\/s13046-018-0864-6","article-title":"LGR5, a novel functional glioma stem cell marker, promotes EMT by activating the Wnt\/beta-catenin pathway and predicts poor survival of glioma patients","volume":"37","author":"Zhang","year":"2018","journal-title":"J. Exp. Clin. Cancer Res."}],"container-title":["Frontiers in Molecular Biosciences"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fmolb.2026.1820992\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T04:34:56Z","timestamp":1786941296000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fmolb.2026.1820992\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,17]]},"references-count":26,"alternative-id":["10.3389\/fmolb.2026.1820992"],"URL":"https:\/\/doi.org\/10.3389\/fmolb.2026.1820992","relation":{},"ISSN":["2296-889X"],"issn-type":[{"value":"2296-889X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,17]]},"article-number":"1820992"}}