{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,29]],"date-time":"2026-06-29T04:45:57Z","timestamp":1782708357255,"version":"3.54.5"},"reference-count":36,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,6,29]],"date-time":"2026-06-29T00:00:00Z","timestamp":1782691200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Mol. Biosci."],"abstract":"<jats:p>\n                    Skin aging is driven by the progressive accumulation of oxidative stress and chronic inflammation, leading to fibroblast dysfunction, extracellular matrix degradation, and impaired tissue repair.\n                    <jats:italic>Centella asiatica<\/jats:italic>\n                    L. is a botanical source rich in triterpenes with documented antioxidant, anti-inflammatory, and collagen-modulating activities; however, its biological efficacy is often limited by poor solubility and bioavailability. The present study aimed to evaluate the effects of a standardized\n                    <jats:italic>Centella asiatica<\/jats:italic>\n                    extract formulated with phospholipids (Centextra\u2122) compared with an unformulated dry extract on oxidative stress, inflammatory stimuli and collagen homeostasis in an\n                    <jats:italic>in vitro<\/jats:italic>\n                    model of human dermal fibroblasts. Both products reduced intracellular ROS levels in a dose-dependent manner; however, the phospholipid-formulated extract exhibited significantly greater antioxidant efficacy, decreasing ROS levels to 115%\u2013174% compared with 368% in cells treated with pro-oxidant stimulus (p &amp;lt; 0.0001). Centextra\u2122 also significantly reduced IL-6 (\u223c75% vs. \u223c150% in stimulated cells; p &amp;lt; 0.05) and MMP-1 levels (\u223c155% vs. higher induction in stimulated cells; p &amp;lt; 0.05). Furthermore, Centextra\u2122 restored collagen levels to 85% vs. 73% impaired by inflammation (p &amp;lt; 0.05) and a greater migratory capacity, whereas the unformulated extract showed limited or no activity across these functional assays. Overall, the phospholipid formulation was associated with enhanced biological activity in this\n                    <jats:italic>in vitro<\/jats:italic>\n                    model, potentially reflecting improved cellular interaction or delivery of bioactive compounds. These findings provide a preliminary scientific rationale for the role of phospholipid-formulated\n                    <jats:italic>C. asiatica<\/jats:italic>\n                    in modulating skin-related contexts. Further studies are required to clarify the underlying mechanisms and confirm these findings in more physiologically relevant models.\n                  <\/jats:p>","DOI":"10.3389\/fmolb.2026.1832045","type":"journal-article","created":{"date-parts":[[2026,6,29]],"date-time":"2026-06-29T04:26:02Z","timestamp":1782707162000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["In vitro antioxidant and collagen restoration activities of a standardized Centella asiatica L. extract formulated with phospholipids"],"prefix":"10.3389","volume":"13","author":[{"given":"Paola","family":"Nezi","sequence":"first","affiliation":[{"name":"Botanicals Lab, Fondazione Toscana Life Sciences","place":["Siena, Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Enrica","family":"Vella","sequence":"additional","affiliation":[{"name":"Product Portfolio Office, Indena SpA","place":["Milan, Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonella","family":"Riva","sequence":"additional","affiliation":[{"name":"Product Portfolio Office, Indena SpA","place":["Milan, Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mattia","family":"Cicogni","sequence":"additional","affiliation":[{"name":"Botanicals Lab, Fondazione Toscana Life Sciences","place":["Siena, Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vittoria","family":"Cicaloni","sequence":"additional","affiliation":[{"name":"Botanicals Lab, Fondazione Toscana Life Sciences","place":["Siena, Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Salvini","sequence":"additional","affiliation":[{"name":"Botanicals Lab, Fondazione Toscana Life Sciences","place":["Siena, Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Tinti","sequence":"additional","affiliation":[{"name":"Botanicals Lab, Fondazione Toscana Life Sciences","place":["Siena, Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,6,29]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"53","DOI":"10.1016\/s0975-3575(10)80010-0","article-title":"Antioxidant and DNA damage preventive properties of Centella asiatica (L) Urb","volume":"2","author":"Anand","year":"2010","journal-title":"Pharmacogn. 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