{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T13:30:37Z","timestamp":1789047037297,"version":"build-2803163510"},"reference-count":80,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T00:00:00Z","timestamp":1787270400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Mol. Biosci."],"abstract":"<jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>\n                      This study aims to evaluate the oxidative stress alleviating potential of glucan exopolysaccharide (EPS) using\n                      <jats:italic>in vitro<\/jats:italic>\n                      (biochemical and cell culture) and\n                      <jats:italic>in vivo Caenorhabditis elegans<\/jats:italic>\n                      model systems.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      The\n                      <jats:italic>in vitro<\/jats:italic>\n                      antioxidant potential of EPS from\n                      <jats:italic>Enterococcus hirae<\/jats:italic>\n                      OL616073 of food origin was evaluated using reducing power, ferric-reducing antioxidant power (FRAP) assay, and 2,2-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical-scavenging activity assays. The antioxidant effect of EPS was also evaluated in\n                      <jats:italic>C. elegans<\/jats:italic>\n                      (wild type Bristol N2), a simple multi-cellular eukaryotic model via survival assay, gene expression, DCF-DA staining, reproduction, and pharyngeal pumping assay. Finally, the ROS scavenging activity of EPS was investigated in human colon cancer cells (Caco-2 cell line) by DCF-DA staining and flow cytometry.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results and discussion<\/jats:title>\n                    <jats:p>\n                      The\n                      <jats:italic>in vitro<\/jats:italic>\n                      assays showed the significant antioxidant activity of EPS as evidenced by reducing power, FRAP, and ABTS radical-scavenging activity. In\n                      <jats:italic>C. elegans<\/jats:italic>\n                      , EPS treatment (150 and 300 \u03bcg\/mL) significantly extended the lifespan of wild-type (Bristol N2)\n                      <jats:italic>C. elegans<\/jats:italic>\n                      (\u223c1.21% and \u223c0.83%) compared to control. EPS treatment (150 \u03bcg\/mL) resulted in increased egg-laying capacity and little change in the pharyngeal pumping rate of\n                      <jats:italic>C. elegans<\/jats:italic>\n                      over five consecutive days. In addition, pre-, post-, and co-treatment of EPS (150 \u03bcg\/mL) in the presence or absence of H\n                      <jats:sub>2<\/jats:sub>\n                      O\n                      <jats:sub>2<\/jats:sub>\n                      (1 mM) showed a reduction in ROS generation. Similarly, in the colorectal cancer cell line (Caco-2), high basal intracellular ROS levels were reduced with increasing concentration of EPS via an increase in intracellular antioxidant enzymes.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      EPS from\n                      <jats:italic>E. hirae<\/jats:italic>\n                      OL616073 of food origin exhibited significant antioxidant potential across diverse model systems. These findings highlight its potential as a natural antioxidant ingredient for applications in functional foods, nutraceuticals, and pharmaceutical formulations.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fmolb.2026.1901988","type":"journal-article","created":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T11:40:56Z","timestamp":1787312456000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Glucan exopolysaccharide from Enterococcus hirae OL616073 alleviates oxidative stress and extends lifespan in different model systems"],"prefix":"10.3389","volume":"13","author":[{"given":"Uma Rani","family":"Potunuru","sequence":"first","affiliation":[{"name":"Department of Food Science and Technology, Pondicherry University","place":["Pondicherry, India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Padmapriya","family":"Ramamoorthy","sequence":"additional","affiliation":[{"name":"Department of Food Science and Technology, Pondicherry University","place":["Pondicherry, India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Balasubramanian Chellammal","family":"Muthubharathi","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, Alagappa University","place":["Karaikudi, Tamil Nadu, India"]},{"name":"Biochemistry and Molecular Biology, School of Medicine, University of New Mexico","place":["Albuquerque, NM, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bargavi A.","family":"Meenachi","sequence":"additional","affiliation":[{"name":"Department of Food Science and Technology, Pondicherry University","place":["Pondicherry, India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raksha","family":"Goswami","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, Pondicherry University","place":["Pondicherry, India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Palanisamy Bruntha","family":"Devi","sequence":"additional","affiliation":[{"name":"Department of Biosciences, Manipal University Jaipur","place":["Jaipur, Rajasthan, India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anjali","family":"Mohan","sequence":"additional","affiliation":[{"name":"Department of Food Science and Technology, Pondicherry University","place":["Pondicherry, India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Krishnaswamy","family":"Balamurugan","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, Alagappa University","place":["Karaikudi, Tamil Nadu, India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arunkumar","family":"Dhayalan","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, Pondicherry University","place":["Pondicherry, India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Digambar","family":"Kavitake","sequence":"additional","affiliation":[{"name":"Department of Food Science and Technology, Pondicherry University","place":["Pondicherry, India"]},{"name":"Research and Development Laboratory, Fermiayur LLP Division, Mysore Mercantile Co. 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