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Recent research has found correlations between hearing loss and markers such as TNF-\u03b1. However, the intrinsic molecular mechanism by which TNF-\u03b1 influences aging individuals\u2019 increased risk of hearing loss remains unclear. In this study, we found that TNF-\u03b1 expression gradually increased with age in DBA\/2J mice. We then used recombinant TNF-\u03b1 to upregulate TNF-\u03b1 levels in House Ear Institute-Organ of Corti 1 (HEI-OC1) cells and found that low concentrations of TNF-\u03b1 could activate the nuclear factor kappa B (NF-\u03baB) transcriptional response to mediate hair cell survival, while high concentrations of TNF-\u03b1 could activate the Caspase-3 cascade to mediate hair cell apoptosis, which preliminarily confirmed that a TNF-\u03b1 mediated signaling pathway plays an important role in the pathogenesis of age-related hearing loss.<\/jats:p>","DOI":"10.3389\/fnagi.2022.956503","type":"journal-article","created":{"date-parts":[[2022,9,7]],"date-time":"2022-09-07T09:19:23Z","timestamp":1662542363000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":19,"title":["Tumor necrosis factor-\u03b1 mediated inflammation versus apoptosis in age-related hearing loss"],"prefix":"10.3389","volume":"14","author":[{"given":"Ting","family":"Wu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiamin","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jingjing","family":"Qiu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuwan","family":"Song","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wentao","family":"Guo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Limei","family":"Cui","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xicheng","family":"Song","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yan","family":"Sun","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2022,9,7]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"105","DOI":"10.1002\/jcb.26174","article-title":"Tumor necrosis factor-alpha: Role in development of insulin resistance and pathogenesis of type 2 diabetes mellitus.","volume":"119","author":"Akash","year":"2018","journal-title":"J. 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