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Aging Neurosci."],"abstract":"<jats:p>\n                    The global increase in neurodegenerative disorders such as Alzheimer\u2019s disease has prompted the search for effective non-pharmacological interventions. Chrono-exercise which is the physical training aligned with circadian rhythms has emerged as a novel strategy to strengthen cognitive resilience. This study explores the impact of chrono-exercises, incorporating aerobic, resistance, and combined modalities, performed at the early dark (ZT13) and early light (ZT1) phases in an aluminum chloride (AlCl\n                    <jats:sub>3<\/jats:sub>\n                    )-induced rat model of neurodegeneration. One hundred male Wistar rats aged 2\u20133 months will undergo structured exercise interventions following AlCl\n                    <jats:sub>3<\/jats:sub>\n                    exposure. Neurobehavioural assessments including novel object recognition and open-field tests along with grip strength analysis, biochemical profiling and histological evaluations of brain and muscle tissues will be conducted. We hypothesize that the timing of combined aerobic-resistance exercise critically influences neuroplasticity and cognitive performance. Findings are expected to guide circadian-based exercise prescriptions to counter cognitive decline and enhance brain health in aging populations.\n                  <\/jats:p>","DOI":"10.3389\/fnagi.2026.1775321","type":"journal-article","created":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T05:58:57Z","timestamp":1777442337000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Chrono-combined aerobic-resistance exercises as therapeutic approach to reverse neurodegeneration in rat model: a detailed protocol"],"prefix":"10.3389","volume":"18","author":[{"given":"Muhammad Hafiz Zuhdi","family":"Fairof","sequence":"first","affiliation":[{"name":"Centre for Healthy Ageing and Wellness (H-CARE), Faculty of Health Sciences, UKM","place":["Kuala Lumpur, Malaysia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hussin","family":"Muhammad","sequence":"additional","affiliation":[{"name":"Toxicology and Pharmacology Unit, Herbal Medicine Research Centre, Institute for Medical Research, National Institutes of Health, Ministry of Health","place":["Setia Alam, Malaysia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amirul Hafiz Ahmad","family":"Abdullah","sequence":"additional","affiliation":[{"name":"Faculty of Health Sciences, Center for Toxicology and Health Risk Studies (CORE), UKM","place":["Kuala Lumpur, Malaysia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Farah Wahida","family":"Ibrahim","sequence":"additional","affiliation":[{"name":"Faculty of Health Sciences, Center for Toxicology and Health Risk Studies (CORE), UKM","place":["Kuala Lumpur, Malaysia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Theng Choon","family":"Ooi","sequence":"additional","affiliation":[{"name":"Premier Integrated Labs Sdn. 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