{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T09:11:54Z","timestamp":1785402714290,"version":"3.56.0"},"reference-count":39,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,4,10]],"date-time":"2026-04-10T00:00:00Z","timestamp":1775779200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003718","name":"Korea Institute of Oriental Medicine","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003718","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Aging Neurosci."],"abstract":"<jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>This study aimed to investigate the neuroprotective and memory-enhancing effects of isoflavone-enriched soybean leaves (IESLs) against scopolamine-induced cognitive impairment. While soybean leaves (SLs) are naturally rich in kaempferol glycosides and soyasaponins, IESLs are characterized by significantly elevated isoflavone levels through metabolite farming.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>To assess the efficacy of IESLs, C57BL\/6 mice were orally administered IESLs (6.25 and 18.8\u202fmg isoflavones\/kg\/day) for 5\u202fweeks, with scopolamine (1\u202fmg\/kg, i.p.) administered to induce memory impairment. Spatial learning and memory functions were evaluated using the Morris water maze and passive avoidance tests.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Scopolamine-induced behavioral deficits were significantly restored by IESLs administration in a dose-dependent manner. Mechanistically, IESLs treatment reduced the intracellular accumulation of reactive oxygen species and enhanced the antioxidant defense system. Furthermore, IESLs significantly upregulated the expression of brain-derived neurotrophic factor (BDNF) and activated the cAMP response element-binding protein (CREB) via phosphorylation. Histological analysis also revealed that IESLs suppressed the activation of astrocytes and microglia, thereby attenuating neuroinflammation.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion<\/jats:title>\n                    <jats:p>These findings suggest that IESLs effectively alleviate cognitive impairment by strengthening antioxidant defenses and activating the CREB-BDNF signaling pathway. Consequently, IESLs may serve as a promising therapeutic or preventive candidate for amnesia and neurodegenerative diseases.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fnagi.2026.1787268","type":"journal-article","created":{"date-parts":[[2026,4,10]],"date-time":"2026-04-10T06:04:14Z","timestamp":1775801054000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":2,"title":["Neuroprotective effects of isoflavone-enriched soybean leaves (Glycine Max) on scopolamine-induced memory deficits in C57BL\/6 mice via antioxidative mechanisms"],"prefix":"10.3389","volume":"18","author":[{"given":"Young Ju","family":"Do","sequence":"first","affiliation":[{"name":"Department of Oriental Medicine Research Division, Korea Institute of Oriental Medicine (KIOM)","place":["Daejeon, Republic of Korea"]},{"name":"University of Science and Technology (UST), Korean Convergence Medicine Major KIOM","place":["Daejeon, Republic of Korea"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seo Young","family":"Kim","sequence":"additional","affiliation":[{"name":"University of Science and Technology (UST), Korean Convergence Medicine Major KIOM","place":["Daejeon, Republic of Korea"]},{"name":"Korean Medicine (KM) Application Center, Korea Institute of Oriental Medicine (KIOM)","place":["Daegu, Republic of Korea"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Younghoon","family":"Go","sequence":"additional","affiliation":[{"name":"Korean Medicine (KM) Application Center, Korea Institute of Oriental Medicine (KIOM)","place":["Daegu, Republic of Korea"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong Hyun","family":"Lee","sequence":"additional","affiliation":[{"name":"Division of Applied Life Science (BK21 four), Institute of Agricultural and Life Science (IALS), Gyeongsang National University","place":["Jinju, Republic of Korea"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ki Hun","family":"Park","sequence":"additional","affiliation":[{"name":"Division of Applied Life Science (BK21 four), Institute of Agricultural and Life Science (IALS), Gyeongsang National University","place":["Jinju, Republic of Korea"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tae Woo","family":"Oh","sequence":"additional","affiliation":[{"name":"University of Science and Technology (UST), Korean Convergence Medicine Major KIOM","place":["Daejeon, Republic of Korea"]},{"name":"Korean Medicine (KM) Application Center, Korea Institute of Oriental Medicine (KIOM)","place":["Daegu, Republic of Korea"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,4,10]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1007\/s10787-025-01755-5","article-title":"Empagliflozin and memantine combination ameliorates cognitive impairment in scopolamine+ heavy metal mixture-induced Alzheimer\u2019s disease in rats: role of AMPK\/mTOR, BDNF, BACE-1, neuroinflammation, and oxidative stress","author":"Abdel-Lah","year":"2025","journal-title":"Inflammopharmacology"},{"key":"ref2","doi-asserted-by":"publisher","first-page":"1119","DOI":"10.1093\/jpp\/rgad048","article-title":"Spotlight on coenzyme Q10 in scopolamine-induced Alzheimer's disease: oxidative stress\/PI3K\/AKT\/GSK 3\u03b2\/CREB\/BDNF\/TrKB","volume":"75","author":"Abuelezz","year":"2023","journal-title":"J. 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