{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T05:59:52Z","timestamp":1790315992318,"version":"4.1.0"},"reference-count":54,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T00:00:00Z","timestamp":1790294400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Aging Neurosci."],"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Sarcopenia is characterized by heterogeneous alterations in muscle mass, strength, and physical performance and may involve interactions among the gut microbiota, tryptophan metabolism, and neuromuscular function. However, these biological characteristics across different sarcopenia stages remain incompletely understood. This study investigated gut microbial characteristics, kynurenine-related metabolism, circulating biomarkers, and physical function in older adults with different sarcopenia status.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      In this cross-sectional study, 67 older adults were classified according to the Asian Working Group for Sarcopenia 2019 criteria as non-sarcopenia (NSG,\n                      <jats:italic>n<\/jats:italic>\n                      \u202f=\u202f17), possible sarcopenia (PSG,\n                      <jats:italic>n<\/jats:italic>\n                      \u202f=\u202f20), or sarcopenia (SG,\n                      <jats:italic>n<\/jats:italic>\n                      \u202f=\u202f30). Muscle mass, handgrip strength, and multidimensional physical performance were assessed. Serum C-terminal agrin fragment (CAF), tumor necrosis factor-\u03b1 (TNF-\u03b1), amino acids, kynurenine (KYN), and tryptophan (Trp) were measured, and the KYN\/Trp ratio was calculated. Gut microbiome composition was characterized by shotgun metagenomic sequencing. Differential abundance was evaluated using MaAsLin2 with adjustment for age and body mass index (BMI), and exploratory correlations among microbial genera, metabolites, circulating biomarkers, and physical function were examined with false discovery rate correction.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      ASMI was significantly lower in the SG group than in the NSG and PSG groups, whereas several measures of physical performance differed among groups without a uniform stepwise pattern. No significant differences were observed in alpha diversity, beta diversity, or the Firmicutes\/Bacteroidetes ratio, and sarcopenia group explained only 3% of the variation in overall microbial community composition (PERMANOVA,\n                      <jats:italic>R<\/jats:italic>\n                      <jats:sup>2<\/jats:sup>\n                      \u202f=\u202f0.03,\n                      <jats:italic>p<\/jats:italic>\n                      \u202f=\u202f0.226). Several genus-level associations were nominally significant after adjustment for age and BMI, but none remained significant after BH-FDR correction. After adjustment for age and BMI, serum KYN concentrations and the KYN\/Trp ratio were significantly higher in the SG group than in the NSG group (\n                      <jats:italic>p<\/jats:italic>\n                      \u202f=\u202f0.011 and\n                      <jats:italic>p<\/jats:italic>\n                      \u202f=\u202f0.043, respectively), whereas Trp and the measured amino acids showed no significant pairwise differences. The unadjusted increase in CAF across sarcopenia groups was attenuated after covariate adjustment, and TNF-\u03b1 did not differ significantly among groups. Exploratory correlation analyses identified several FDR-significant associations across microbial, metabolic, and functional domains, including negative associations of KYN and KYN\/Trp with handgrip strength and a positive association between CAF and 6-m gait speed(GS).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Established sarcopenia was associated with altered circulating kynurenine-related metabolism despite the absence of pronounced differences in overall gut microbial community structure. Exploratory cross-domain associations further suggest potential interactions among gut microbial characteristics, tryptophan metabolism, and neuromuscular and physical function. These findings support further investigation of host\u2013microbial tryptophan metabolism in sarcopenia but do not establish a causal microbiota\u2013kynurenine\u2013NMJ pathway.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fnagi.2026.1883616","type":"journal-article","created":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T05:32:01Z","timestamp":1790314321000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Gut microbial characteristics and kynurenine-related metabolism across sarcopenia groups: associations with neuromuscular and physical function"],"prefix":"10.3389","volume":"18","author":[{"given":"Chang","family":"Fang","sequence":"first","affiliation":[{"name":"School of Sports Medicine and Rehabilitation, Beijing Sport University","place":["Beijing, China"]},{"name":"College of Sports and Exercise Rehabilitation, Jinzhou Medical University","place":["Jinzhou, Liaoning, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chen","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Sports Medicine and Rehabilitation, Beijing Sport University","place":["Beijing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rui","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Sports Medicine and Rehabilitation, Beijing Sport University","place":["Beijing, China"]},{"name":"School of Medical Humanities, Anhui Medical University","place":["Hefei, Anhui, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi-fan","family":"Lu","sequence":"additional","affiliation":[{"name":"School of Sports Medicine and Rehabilitation, Beijing Sport University","place":["Beijing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ling","family":"Ma","sequence":"additional","affiliation":[{"name":"College of Sports and Exercise Rehabilitation, Jinzhou Medical University","place":["Jinzhou, Liaoning, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,9,25]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"33","DOI":"10.1016\/j.cell.2014.07.051","article-title":"Skeletal muscle PGC-1\u03b11 modulates kynurenine metabolism and mediates resilience to stress-induced depression","volume":"159","author":"Agudelo","year":"2014","journal-title":"Cell"},{"key":"ref2","doi-asserted-by":"publisher","first-page":"716","DOI":"10.1016\/j.chom.2018.05.003","article-title":"Gut microbiota regulation of tryptophan metabolism in health and disease","volume":"23","author":"Agus","year":"2018","journal-title":"Cell Host Microbe"},{"key":"ref3","doi-asserted-by":"publisher","first-page":"197","DOI":"10.3390\/cells9010197","article-title":"Mitochondrial mechanisms of neuromuscular junction degeneration with aging","volume":"9","author":"Anagnostou","year":"2020","journal-title":"Cells"},{"key":"ref4","doi-asserted-by":"publisher","first-page":"15039","DOI":"10.3390\/ijms232315039","article-title":"Inflammaging: implications in sarcopenia","volume":"23","author":"Antu\u00f1a","year":"2022","journal-title":"Int. J. Mol. Sci."},{"key":"ref5","doi-asserted-by":"publisher","first-page":"3132","DOI":"10.3390\/nu15143132","article-title":"The role of the kynurenine pathway in the pathophysiology of frailty, sarcopenia, and osteoporosis","volume":"15","author":"Ballesteros","year":"2023","journal-title":"Nutrients"},{"key":"ref6","doi-asserted-by":"publisher","first-page":"597811","DOI":"10.3389\/fnagi.2020.597811","article-title":"AChRs degeneration at NMJ in aging-associated sarcopenia\u2014a systematic review","volume":"12","author":"Bao","year":"2020","journal-title":"Front. Aging Neurosci."},{"key":"ref7","doi-asserted-by":"publisher","first-page":"755","DOI":"10.1093\/oxfordjournals.aje.a009520","article-title":"Epidemiology of sarcopenia among the elderly in New Mexico","volume":"147","author":"Baumgartner","year":"1998","journal-title":"Am. J. Epidemiol."},{"key":"ref8","doi-asserted-by":"publisher","first-page":"S350","DOI":"10.1002\/acr.20538","article-title":"Measures of physical performance assessments: self-paced walk test (SPWT), stair climb test (SCT), six-minute walk test (6MWT), chair stand test (CST), timed up & go (TUG), sock test, lift and carry test (LCT), and car task","volume":"63","author":"Bennell","year":"2011","journal-title":"Arthritis Care Res."},{"key":"ref9","doi-asserted-by":"publisher","first-page":"889","DOI":"10.1186\/s12877-022-03601-7","article-title":"Prevalence of sarcopenia under different diagnostic criteria and the changes in muscle mass, muscle strength, and physical function with age in Chinese old adults","volume":"22","author":"Cao","year":"2022","journal-title":"BMC Geriatr."},{"key":"ref10","doi-asserted-by":"publisher","first-page":"1947","DOI":"10.4103\/NRR.NRR-D-24-01338","article-title":"Systematic review of mitochondrial dysfunction and oxidative stress in aging: a focus on neuromuscular junctions","volume":"21","author":"Chai","year":"2026","journal-title":"Neural Regen. Res."},{"key":"ref11","doi-asserted-by":"publisher","first-page":"300","DOI":"10.1016\/j.jamda.2019.12.012","article-title":"Asian working Group for Sarcopenia: 2019 consensus update on sarcopenia diagnosis and treatment","volume":"21","author":"Chen","year":"2020","journal-title":"J. Am. Med. Dir. Assoc."},{"key":"ref12","doi-asserted-by":"publisher","first-page":"135","DOI":"10.1186\/s12974-021-02175-2","article-title":"Tryptophan-kynurenine metabolism: a link between the gut and brain for depression in inflammatory bowel disease","volume":"18","author":"Chen","year":"2021","journal-title":"J. Neuroinflammation"},{"key":"ref13","doi-asserted-by":"publisher","first-page":"114056","DOI":"10.1016\/j.intimp.2025.114056","article-title":"The role of chronic low-grade inflammation in the development of sarcopenia: advances in molecular mechanisms","volume":"147","author":"Cheng","year":"2025","journal-title":"Int. Immunopharmacol."},{"key":"ref14","doi-asserted-by":"publisher","first-page":"1486793","DOI":"10.3389\/fendo.2024.1486793","article-title":"A systematic review on gut microbiota in type 2 diabetes mellitus","volume":"15","author":"Chong","year":"2025","journal-title":"Front. Endocrinol."},{"key":"ref15","doi-asserted-by":"publisher","first-page":"e13849","DOI":"10.1111\/acel.13849","article-title":"Deletion of quinolinate phosphoribosyltransferase gene accelerates frailty phenotypes and neuromuscular decline with aging in a sex-specific pattern","volume":"22","author":"Chung","year":"2023","journal-title":"Aging Cell"},{"key":"ref16","doi-asserted-by":"publisher","first-page":"412","DOI":"10.1093\/ageing\/afq034","article-title":"Sarcopenia: European consensus on definition and diagnosis: report of the European working group on sarcopenia in older people","volume":"39","author":"Cruz-Jentoft","year":"2010","journal-title":"Age Ageing"},{"key":"ref17","doi-asserted-by":"publisher","first-page":"16","DOI":"10.1093\/ageing\/afy169","article-title":"Sarcopenia: revised European consensus on definition and diagnosis","volume":"48","author":"Cruz-Jentoft","year":"2019","journal-title":"Age Ageing"},{"key":"ref18","doi-asserted-by":"publisher","first-page":"1045","DOI":"10.3389\/fphys.2017.01045","article-title":"The role of inflammation in age-related sarcopenia","volume":"8","author":"Dalle","year":"2017","journal-title":"Front. Physiol."},{"key":"ref19","doi-asserted-by":"publisher","first-page":"45","DOI":"10.1080\/08830185.2021.1996573","article-title":"Lipopolysaccharides derived from gram-negative bacterial pool of human gut microbiota promote inflammation and obesity development","volume":"41","author":"Du","year":"2022","journal-title":"Int. Rev. Immunol."},{"key":"ref20","doi-asserted-by":"publisher","first-page":"afae113","DOI":"10.1093\/ageing\/afae113","article-title":"A systematic review of the psychometric properties of physical performance tests for sarcopenia in community-dwelling older adults","volume":"53","author":"Exter","year":"2024","journal-title":"Age Ageing"},{"key":"ref21","doi-asserted-by":"publisher","first-page":"e13707","DOI":"10.1002\/jcsm.13707","article-title":"C-terminal agrin fragment as a biomarker for sarcopenia: a systematic review and meta-analysis","volume":"16","author":"Fatima","year":"2025","journal-title":"J. Cachexia. 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Physiol."},{"key":"ref25","doi-asserted-by":"publisher","first-page":"66","DOI":"10.1186\/s12986-024-00836-6","article-title":"Gut matters in microgravity: potential link of gut microbiota and its metabolites to cardiovascular and musculoskeletal well-being","volume":"21","author":"Ibrahim","year":"2024","journal-title":"Nutr. Metab."},{"key":"ref26","doi-asserted-by":"publisher","first-page":"e0047924","DOI":"10.1128\/jb.00479-24","article-title":"Unraveling the gut microbiota\u2019s role in obesity: key metabolites, microbial species, and therapeutic insights","volume":"207","author":"Iqbal","year":"2025","journal-title":"J. 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Microbiol."},{"key":"ref30","doi-asserted-by":"publisher","first-page":"11185","DOI":"10.3390\/ijms262211185","article-title":"Neural cues and genomic clues: NGS insights into neurogenic sarcopenia and muscle atrophy","volume":"26","author":"Kupriyanova","year":"2025","journal-title":"Int. J. Mol. Sci."},{"key":"ref31","doi-asserted-by":"publisher","first-page":"83","DOI":"10.11005\/jbm.2017.24.2.83","article-title":"Sarcopenia: neurological point of view","volume":"24","author":"Kwon","year":"2017","journal-title":"J. Bone Metab."},{"key":"ref32","doi-asserted-by":"publisher","first-page":"31","DOI":"10.1016\/j.exger.2016.03.012","article-title":"Serum levels of C-terminal agrin fragment (CAF) are associated with sarcopenia in older multimorbid community-dwellers: results from the ilSIRENTE study","volume":"79","author":"Landi","year":"2016","journal-title":"Exp. Gerontol."},{"key":"ref33","doi-asserted-by":"publisher","first-page":"348","DOI":"10.14283\/jfa.2022.59","article-title":"Singapore clinical practice guidelines for sarcopenia: screening, diagnosis, management and prevention","volume":"11","author":"Lim","year":"2022","journal-title":"J. Frailty Aging"},{"key":"ref34","doi-asserted-by":"publisher","first-page":"79","DOI":"10.1016\/j.exger.2014.10.003","article-title":"Serum levels of C-terminal agrin fragment (CAF) are associated with sarcopenia in older hip fractured patients","volume":"60","author":"Marzetti","year":"2014","journal-title":"Exp. Gerontol."},{"key":"ref35","doi-asserted-by":"publisher","first-page":"549","DOI":"10.2147\/CIA.S44112","article-title":"Age-related decrease in physical activity and functional fitness among elderly men and women","volume":"8","author":"Milanovi\u0107","year":"2013","journal-title":"Clin. Interv. Aging"},{"key":"ref36","doi-asserted-by":"publisher","first-page":"1619","DOI":"10.3945\/jn.109.104638","article-title":"Butyrate enhances the intestinal barrier by facilitating tight junction assembly via activation of AMP-activated protein kinase in Caco-2 cell monolayers","volume":"139","author":"Peng","year":"2009","journal-title":"J. Nutr."},{"key":"ref37","doi-asserted-by":"publisher","first-page":"242","DOI":"10.1186\/s12877-020-01642-4","article-title":"The diagnostic value of the short physical performance battery for sarcopenia","volume":"20","author":"Phu","year":"2020","journal-title":"BMC Geriatr."},{"key":"ref38","doi-asserted-by":"publisher","first-page":"485","DOI":"10.1007\/s10522-015-9627-3","article-title":"Age-dependent motor unit remodelling in human limb muscles","volume":"17","author":"Piasecki","year":"2016","journal-title":"Biogerontology"},{"key":"ref39","doi-asserted-by":"publisher","first-page":"576","DOI":"10.1093\/gerona\/glaa207","article-title":"Neuromuscular junction aging: a role for biomarkers and exercise","volume":"76","author":"Pratt","year":"2021","journal-title":"J. Gerontol. A Biol. Sci. Med. Sci."},{"key":"ref40","doi-asserted-by":"publisher","first-page":"606986","DOI":"10.3389\/fcimb.2021.606986","article-title":"Lipopolysaccharide preparation derived from Porphyromonas gingivalis induces a weaker immuno-inflammatory response in BV-2 microglial cells than Escherichia coli by differentially activating TLR2\/4-mediated NF-\u03baB\/STAT3 signaling pathways","volume":"11","author":"Qiu","year":"2021","journal-title":"Front. Cell. Infect. Microbiol."},{"key":"ref41","doi-asserted-by":"publisher","first-page":"3294","DOI":"10.1038\/s41467-018-05470-4","article-title":"Microbial tryptophan catabolites in health and disease","volume":"9","author":"Roager","year":"2018","journal-title":"Nat. Commun."},{"key":"ref42","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1097\/MCO.0000000000000267","article-title":"Neuromuscular junction degeneration in muscle wasting","volume":"19","author":"Rudolf","year":"2016","journal-title":"Curr. Opin. Clin. Nutr. Metab. Care"},{"key":"ref43","doi-asserted-by":"publisher","first-page":"38","DOI":"10.4235\/agmr.24.0131","article-title":"Impact of sarcopenia on falls, mobility limitation, and mortality using the diagnostic criteria proposed in the Korean working group on sarcopenia guideline","volume":"29","author":"Shim","year":"2025","journal-title":"Ann. Geriatr. Med. Res."},{"key":"ref44","doi-asserted-by":"publisher","first-page":"16731","DOI":"10.1073\/pnas.0804812105","article-title":"Faecalibacterium prausnitzii is an anti-inflammatory commensal bacterium identified by gut microbiota analysis of Crohn disease patients","volume":"105","author":"Sokol","year":"2008","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref45","doi-asserted-by":"publisher","first-page":"129","DOI":"10.3390\/ijms27010129","article-title":"Kynurenine pathway metabolites as mediators of exercise-induced mood enhancement, fatigue resistance, and neuroprotection","volume":"27","author":"Tero-Vescan","year":"2025","journal-title":"Int. J. Mol. Sci."},{"key":"ref46","doi-asserted-by":"publisher","first-page":"1303","DOI":"10.3390\/nu9121303","article-title":"Aging gut microbiota at the cross-road between nutrition, physical frailty, and sarcopenia: Is there a gut-muscle axis?","volume":"9","author":"Ticinesi","year":"2017","journal-title":"Nutrients"},{"key":"ref47","doi-asserted-by":"publisher","first-page":"296","DOI":"10.1016\/j.immuni.2014.06.014","article-title":"Symbiotic bacterial metabolites regulate gastrointestinal barrier function via the xenobiotic sensor PXR and toll-like receptor 4","volume":"41","author":"Venkatesh","year":"2014","journal-title":"Immunity"},{"key":"ref48","doi-asserted-by":"publisher","first-page":"e21171","DOI":"10.1096\/fj.202001158R","article-title":"Porphyromonas gingivalis impairs glucose uptake in skeletal muscle associated with altering gut microbiota","volume":"35","author":"Watanabe","year":"2021","journal-title":"FASEB J."},{"key":"ref49","doi-asserted-by":"publisher","first-page":"e136091","DOI":"10.1172\/jci.insight.136091","article-title":"Kynurenines link chronic inflammation to functional decline and physical frailty","volume":"5","author":"Westbrook","year":"2020","journal-title":"JCI Insight"},{"key":"ref50","doi-asserted-by":"publisher","first-page":"glae044","DOI":"10.1093\/gerona\/glae044","article-title":"Effect of gut microbiota-mediated tryptophan metabolism on inflammaging in frailty and sarcopenia","volume":"79","author":"Xu","year":"2024","journal-title":"J. Gerontol. A Biol. Sci. Med. Sci."},{"key":"ref51","doi-asserted-by":"publisher","first-page":"1304","DOI":"10.1016\/j.cmet.2023.06.004","article-title":"Tryptophan metabolism in health and disease","volume":"35","author":"Xue","year":"2023","journal-title":"Cell Metab."},{"key":"ref52","doi-asserted-by":"publisher","first-page":"372","DOI":"10.1016\/j.immuni.2013.08.003","article-title":"Tryptophan catabolites from microbiota engage aryl hydrocarbon receptor and balance mucosal reactivity via interleukin-22","volume":"39","author":"Zelante","year":"2013","journal-title":"Immunity"},{"key":"ref53","doi-asserted-by":"publisher","first-page":"99","DOI":"10.1177\/1753425907088244","article-title":"Lipopolysaccharide (LPS) of Porphyromonas gingivalis induces IL-1\u03b2, TNF-\u03b1 and IL-6 production by THP-1 cells in a way different from that of Escherichia coli LPS","volume":"14","author":"Zhang","year":"2008","journal-title":"Innate Immun."},{"key":"ref54","doi-asserted-by":"publisher","first-page":"1267","DOI":"10.2147\/NDT.S301064","article-title":"Handgrip strength and depression among older Chinese inpatients: a cross-sectional study","volume":"17","author":"Zhang","year":"2021","journal-title":"Neuropsychiatr. Dis. Treat."}],"container-title":["Frontiers in Aging Neuroscience"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fnagi.2026.1883616\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T05:32:02Z","timestamp":1790314322000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fnagi.2026.1883616\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,25]]},"references-count":54,"alternative-id":["10.3389\/fnagi.2026.1883616"],"URL":"https:\/\/doi.org\/10.3389\/fnagi.2026.1883616","relation":{},"ISSN":["1663-4365"],"issn-type":[{"value":"1663-4365","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,25]]},"article-number":"1883616"}}