{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T23:20:37Z","timestamp":1792624837451,"version":"4.5.0"},"reference-count":43,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2020,2,5]],"date-time":"2020-02-05T00:00:00Z","timestamp":1580860800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100014718","name":"Innovative Research Group Project of the National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81970884, 81900941, 81900944, 81622013, 81970882, 81570919, 81870721, 81771019, 81700913, 81670928, 81570921"],"award-info":[{"award-number":["81970884, 81900941, 81900944, 81622013, 81970882, 81570919, 81870721, 81771019, 81700913, 81670928, 81570921"]}],"id":[{"id":"10.13039\/100014718","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004608","name":"Natural Science Foundation of Jiangsu Province","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004608","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012692","name":"K. C. Wong Education Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100012692","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell. Neurosci."],"DOI":"10.3389\/fncel.2019.00590","type":"journal-article","created":{"date-parts":[[2020,2,5]],"date-time":"2020-02-05T13:21:06Z","timestamp":1580908866000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":72,"title":["Blebbistatin Inhibits Neomycin-Induced Apoptosis in Hair Cell-Like HEI-OC-1 Cells and in Cochlear Hair Cells"],"prefix":"10.3389","volume":"13","author":[{"given":"Song","family":"Gao","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cheng","family":"Cheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maohua","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pei","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liyan","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ya","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huihui","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuanfu","family":"Zeng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xia","family":"Gao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongming","family":"Ma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Renjie","family":"Chai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2020,2,5]]},"reference":[{"key":"B34","doi-asserted-by":"publisher","first-page":"483","DOI":"10.1016\/j.cell.2005.02.001","article-title":"Mitochondria, oxidants, and aging","volume":"120","author":"Balaban","year":"2005","journal-title":"Cell"},{"key":"B2","doi-asserted-by":"publisher","first-page":"105","DOI":"10.1021\/cb3005116","article-title":"Aminoglycoside antibiotics in the 21st century","volume":"8","author":"Becker","year":"2013","journal-title":"ACS Chem. Biol."},{"key":"B5","doi-asserted-by":"publisher","first-page":"e1605","DOI":"10.1038\/cddis.2014.549","article-title":"Bmi1 regulates auditory hair cell survival by maintaining redox balance","volume":"6","author":"Chen","year":"2015","journal-title":"Cell Death Dis."},{"key":"B3","doi-asserted-by":"publisher","first-page":"2189","DOI":"10.1042\/cs20180294","article-title":"Blebbistatin modulates prostatic cell growth and contrapctility through myosin II signaling","volume":"132","author":"Chen","year":"2018","journal-title":"Clin. Sci."},{"key":"B6","doi-asserted-by":"publisher","first-page":"645","DOI":"10.1007\/s10162-013-0404-1","article-title":"Bax, Bcl2, and p53 differentially regulate neomycin- and gentamicin-induced hair cell death in the zebrafish lateral line","volume":"14","author":"Coffin","year":"2013","journal-title":"J. Assoc. Res. Otolaryngol."},{"key":"B7","doi-asserted-by":"publisher","first-page":"201","DOI":"10.1016\/j.ijantimicag.2008.09.001","article-title":"Do we still need the aminoglycosides?","volume":"33","author":"Durante-Mangoni","year":"2009","journal-title":"Int. J. Antimicrob. Agents"},{"key":"B9","doi-asserted-by":"publisher","first-page":"3556","DOI":"10.1172\/jci84939","article-title":"Mitochondrial calcium uptake underlies ROS generation during aminoglycoside-induced hair cell death","volume":"126","author":"Esterberg","year":"2016","journal-title":"J. Clin. Invest."},{"key":"B10","doi-asserted-by":"publisher","first-page":"66647","DOI":"10.18632\/oncotarget.11336","article-title":"Inhibition of ARC decreases the survival of HEI-OC-1 cells after neomycin damage in vitro","volume":"7","author":"Guan","year":"2016","journal-title":"Oncotarget"},{"key":"B11","doi-asserted-by":"publisher","first-page":"17","DOI":"10.1007\/978-981-13-6123-4_2","article-title":"Protection of hair cells from ototoxic drug-induced hearing loss","volume":"1130","author":"Guo","year":"2019","journal-title":"Adv. Exp. Med. Biol."},{"key":"B13","doi-asserted-by":"publisher","first-page":"1884","DOI":"10.1080\/15548627.2017.1359449","article-title":"Autophagy protects auditory hair cells against neomycin-induced damage","volume":"13","author":"He","year":"2017","journal-title":"Autophagy"},{"key":"B14","doi-asserted-by":"publisher","first-page":"29621","DOI":"10.1038\/srep29621","article-title":"Reduced TRMU expression increases the sensitivity of hair-cell-like HEI-OC-1 cells to neomycin damage in vitro","volume":"6","author":"He","year":"2016","journal-title":"Sci. Rep."},{"key":"B15","doi-asserted-by":"publisher","first-page":"11","DOI":"10.1016\/j.heares.2007.11.002","article-title":"The impact of mitochondrial energetic dysfunction on apoptosis in outer hair cells of the cochlea following exposure to intense noise","volume":"236","author":"Hu","year":"2008","journal-title":"Hear. Res."},{"key":"B16","doi-asserted-by":"publisher","first-page":"e50231","DOI":"10.7554\/eLife.50231","article-title":"Release of cholesterol-rich particles from the macrophage plasma membrane during movement of filopodia and lamellipodia","volume":"8","author":"Hu","year":"2019","journal-title":"Elife"},{"key":"B17","doi-asserted-by":"publisher","first-page":"259","DOI":"10.1016\/s0736-5748(99)00094-5","article-title":"Oxidative stress-induced apoptosis of cochlear sensory cells: otoprotective strategies","volume":"18","author":"Huang","year":"2000","journal-title":"Int. J. Dev. Neurosci."},{"key":"B18","doi-asserted-by":"publisher","first-page":"308","DOI":"10.3389\/fncel.2017.00308","article-title":"Aminoglycoside-induced cochleotoxicity: a review","volume":"11","author":"Jiang","year":"2017","journal-title":"Front. Cell. Neurosci."},{"key":"B19","doi-asserted-by":"publisher","first-page":"1449","DOI":"10.1097\/mao.0000000000001192","article-title":"Role of STAT1 and oxidative stress in gentamicin-induced hair cell death in organ of corti","volume":"37","author":"Jiang","year":"2016","journal-title":"Otol. Neurotol."},{"key":"B21","doi-asserted-by":"publisher","first-page":"38","DOI":"10.1016\/j.abb.2016.08.021","article-title":"Recent developments in detection of superoxide radical anion and hydrogen peroxide: opportunities, challenges, and implications in redox signaling","volume":"617","author":"Kalyanaraman","year":"2017","journal-title":"Arch. Biochem. Biophys."},{"key":"B22","doi-asserted-by":"publisher","first-page":"2871","DOI":"10.1242\/jcs.023705","article-title":"TRPV4 enhances the cellular uptake of aminoglycoside antibiotics","volume":"121","author":"Karasawa","year":"2008","journal-title":"J. Cell Sci."},{"key":"B23","doi-asserted-by":"publisher","first-page":"35557","DOI":"10.1074\/jbc.M405319200","article-title":"Mechanism of blebbistatin inhibition of myosin II","volume":"279","author":"Kov\u00e1cs","year":"2004","journal-title":"J. Biol. Chem."},{"key":"B24","doi-asserted-by":"publisher","first-page":"C490","DOI":"10.1152\/ajpcell.00043.2011","article-title":"Inhibition of suicidal erythrocyte death by blebbistatin","volume":"301","author":"Lang","year":"2011","journal-title":"Am. J. Physiol. Cell Physiol."},{"key":"B26","doi-asserted-by":"publisher","first-page":"211","DOI":"10.1016\/j.ijcard.2018.04.079","article-title":"Inhibition of myosin IIA-actin interaction prevents ischemia\/reperfusion induced cardiomyocytes apoptosis through modulating PINK1\/Parkin pathway and mitochondrial fission","volume":"271","author":"Li","year":"2018","journal-title":"Int. J. Cardiol."},{"key":"B27","doi-asserted-by":"publisher","first-page":"139","DOI":"10.3389\/fncel.2018.00139","article-title":"Meclofenamic acid reduces reactive oxygen species accumulation and apoptosis, inhibits excessive autophagy, and protects hair cell-like HEI-OC1 cells from cisplatin-induced damage","volume":"12","author":"Li","year":"2018","journal-title":"Front. Cell. Neurosci."},{"key":"B25","doi-asserted-by":"publisher","first-page":"1033","DOI":"10.1080\/10717544.2018.1461277","article-title":"Novel compounds protect auditory hair cells against gentamycin-induced apoptosis by maintaining the expression level of H3K4me2","volume":"25","author":"Li","year":"2018","journal-title":"Drug Deliv."},{"key":"B28","doi-asserted-by":"publisher","first-page":"e2136","DOI":"10.1038\/cddis.2016.35","article-title":"Wnt activation protects against neomycin-induced hair cell damage in the mouse cochlea","volume":"7","author":"Liu","year":"2016","journal-title":"Cell Death Dis."},{"key":"B29","doi-asserted-by":"publisher","first-page":"1389","DOI":"10.1089\/ars.2017.7288","article-title":"Wnt signaling activates TIGAR and protects against cisplatin-induced spiral ganglion neuron damage in the mouse cochlea","volume":"30","author":"Liu","year":"2019","journal-title":"Antioxid. Redox Signal."},{"key":"B30","doi-asserted-by":"publisher","first-page":"188","DOI":"10.1002\/path.2102","article-title":"Ageing and hearing loss","volume":"211","author":"Liu","year":"2007","journal-title":"J. Pathol."},{"key":"B31","doi-asserted-by":"publisher","first-page":"505","DOI":"10.1113\/jphysiol.2005.085951","article-title":"The aminoglycoside antibiotic dihydrostreptomycin rapidly enters mouse outer hair cells through the mechano-electrical transducer channels","volume":"567","author":"Marcotti","year":"2010","journal-title":"J. Physiol."},{"key":"B32","doi-asserted-by":"publisher","first-page":"1349","DOI":"10.1007\/s00424-018-2152-x","article-title":"Regional increase in ROS within stretched region exacerbates arrhythmias in rat trabeculae with nonuniform contraction","volume":"470","author":"Miura","year":"2018","journal-title":"Pflugers Arch."},{"key":"B20","doi-asserted-by":"publisher","first-page":"1092","DOI":"10.1056\/NEJM199310073291507","article-title":"Hearing loss","volume":"329","author":"Nadol","year":"1993","journal-title":"N. Eng. J. Med."},{"key":"B33","doi-asserted-by":"publisher","first-page":"8181","DOI":"10.1038\/srep08181","article-title":"Adjudin protects rodent cochlear hair cells against gentamicin ototoxicity via the SIRT3-ROS pathway","volume":"5","author":"Quan","year":"2015","journal-title":"Sci. Rep."},{"key":"B35","doi-asserted-by":"publisher","first-page":"36273","DOI":"10.1074\/jbc.m507518200","article-title":"2-cyano-3,12-dioxooleana-1,9-dien-28-imidazolide (CDDO-Im) directly targets mitochondrial glutathione to induce apoptosis in pancreatic cancer","volume":"280","author":"Samudio","year":"2005","journal-title":"J. Biol. Chem."},{"key":"B36","doi-asserted-by":"publisher","first-page":"161","DOI":"10.1016\/s0196-0709(00)80003-4","article-title":"Biologic activity of mitochondrial metabolites on aging and age-related hearing loss","volume":"21","author":"Seidman","year":"2000","journal-title":"Am. J. Otol."},{"key":"B37","doi-asserted-by":"publisher","first-page":"158","DOI":"10.1016\/j.molcel.2012.09.025","article-title":"Physiological roles of mitochondrial reactive oxygen species","volume":"48","author":"Sena","year":"2012","journal-title":"Mol. Cell"},{"key":"B38","doi-asserted-by":"publisher","first-page":"778","DOI":"10.1038\/nrm2786","article-title":"Non-muscle myosin II takes centre stage in cell adhesion and migration","volume":"10","author":"Vicente-Manzanares","year":"2009","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"B39","doi-asserted-by":"publisher","first-page":"1186","DOI":"10.1038\/s41467-019-09121-0","article-title":"Actomyosin contractility-dependent matrix stretch and recoil induces rapid cell migration","volume":"10","author":"Wang","year":"2019","journal-title":"Nat. Commun."},{"key":"B40","doi-asserted-by":"publisher","first-page":"140","DOI":"10.1093\/cvr\/cvw023","article-title":"MicroRNA-10a\/10b represses a novel target gene mib1 to regulate angiogenesis","volume":"110","author":"Wang","year":"2016","journal-title":"Cardiovasc. Res."},{"key":"B41","doi-asserted-by":"publisher","first-page":"75","DOI":"10.3389\/fnmol.2017.00075","article-title":"Myosin IIA-related actomyosin contractility mediates oxidative stress-induced neuronal apoptosis","volume":"10","author":"Wang","year":"2017","journal-title":"Front. Mol. Neurosci."},{"key":"B42","doi-asserted-by":"publisher","first-page":"3506","DOI":"10.1038\/s41598-017-03810-w","article-title":"Bone morphogenetic protein 4 promotes the survival and preserves the structure of flow-sorted Bhlhb5+ cochlear spiral ganglion neurons in vitro","volume":"7","author":"Waqas","year":"2017","journal-title":"Sci. Rep."},{"key":"B43","doi-asserted-by":"publisher","first-page":"1974","DOI":"10.1091\/mbc.e19-02-0076","article-title":"Myosin IIA-mediated forces regulate multicellular integrity during vascular sprouting","volume":"30","author":"Yoon","year":"2019","journal-title":"Mol. Biol. Cell"},{"key":"B44","doi-asserted-by":"publisher","first-page":"41094","DOI":"10.1038\/srep41094","article-title":"c-Myb knockdown increases the neomycin-induced damage to hair-cell-like HEI-OC1 cells in vitro","volume":"7","author":"Yu","year":"2017","journal-title":"Sci. Rep."},{"key":"B45","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-019-03291-2","article-title":"Knockdown of Foxg1 in supporting cells increases the trans-differentiation of supporting cells into hair cells in the neonatal mouse cochlea","author":"Zhang","year":"2019","journal-title":"Cell. Mol. Life Sci."},{"key":"B46","doi-asserted-by":"publisher","first-page":"7166","DOI":"10.1038\/ncomms8166","article-title":"The non-mu scle-myosin-II heavy chain Myh9 mediates colitis-induced epithelium injury by restricting Lgr5+ stem cells","volume":"6","author":"Zhao","year":"2015","journal-title":"Nat. Commun."},{"key":"B47","doi-asserted-by":"publisher","first-page":"3","DOI":"10.1038\/jp.2012.105","article-title":"Ototoxicity in preterm infants: effects of genetics, aminoglycosides, and loud environmental noise","volume":"33","author":"Zimmerman","year":"2013","journal-title":"J. Perinatol."}],"container-title":["Frontiers in Cellular Neuroscience"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/article\/10.3389\/fncel.2019.00590\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,2,5]],"date-time":"2020-02-05T13:21:09Z","timestamp":1580908869000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/article\/10.3389\/fncel.2019.00590\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,2,5]]},"references-count":43,"alternative-id":["10.3389\/fncel.2019.00590"],"URL":"https:\/\/doi.org\/10.3389\/fncel.2019.00590","relation":{},"ISSN":["1662-5102"],"issn-type":[{"value":"1662-5102","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,2,5]]},"article-number":"590"}}