{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T04:56:31Z","timestamp":1790571391975,"version":"4.1.0"},"reference-count":28,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T00:00:00Z","timestamp":1790553600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell. Neurosci."],"abstract":"<jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>Peripheral nerve injuries can produce regenerative or persistent-disconnection outcomes depending on the injury modality, but the underlying cellular responses and their effects on the central nervous system remain incompletely defined.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We compared sciatic nerve crush (SNC; axonotmesis) and sciatic nerve transection (SNT; neurotmesis) in wild-type, AdvCre;Ai14, and MYCN-eGFP reporter mice. Longitudinal assessments included functional recovery, neuronal injury responses marked by ATF3, IB4-positive sensory-neuron responses, dorsal-horn microglial activation, and spinal MYCN-eGFP signal.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>SNC was associated with partial, measure-specific functional recovery, a transient and self-limited ATF3 response, and a limited reduction in IB4-positive neuronal profiles. In contrast, SNT produced persistent axotomy-associated neuronal stress, a reduction in IB4-positive neuronal profiles, and more protracted dorsal-horn microglial activation. MYCN-eGFP intensity was significantly increased above sham levels bilaterally in spinal ventral-horn neurons after both SNC and SNT at postoperative day 7 (POD7), with the greatest acute amplitude observed after SNC. From POD7 to POD14, MYCN-eGFP intensity declined by more than 90% after SNC but by approximately 60% after SNT, indicating distinct temporal patterns between the two injury modalities.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion<\/jats:title>\n                    <jats:p>SNC and SNT engage overlapping but quantitatively and temporally distinct cellular programs, including modality-specific dorsal root ganglion neuronal responses associated with functional recovery. These findings refine the interpretation of two widely used peripheral nerve injury models and identify bilateral spinal MYCN-eGFP dynamics as a candidate cellular readout for future mechanistic investigation.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fncel.2026.1922426","type":"journal-article","created":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T04:29:46Z","timestamp":1790569786000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Divergent cellular responses to sciatic nerve crush versus transection: bilateral MYCN induction and distinct DRG neuronal responses"],"prefix":"10.3389","volume":"20","author":[{"given":"Shilian","family":"Zhou","sequence":"first","affiliation":[{"name":"Guangdong Cardiovascular Institute, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences","place":["Guangzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ziyan","family":"Huang","sequence":"additional","affiliation":[{"name":"Guangdong Cardiovascular Institute, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences","place":["Guangzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhongyuan","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology, First Affiliated Hospital, Sun Yat-sen University","place":["Guangzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingwei","family":"Chen","sequence":"additional","affiliation":[{"name":"Dermatology Hospital, Southern Medical University","place":["Guangzhou, China"]},{"name":"The First School of Clinical Medicine, Southern Medical University","place":["Guangzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinyu","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Philosophy, Sun Yat-sen University","place":["Guangzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diya","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Biomedical Information Engineering of Ministry of Education, Department of Biomedical Engineering, School of Life Science and Technology, Xi'an Jiaotong University","place":["Xi'an, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haihua","family":"Shu","sequence":"additional","affiliation":[{"name":"Guangdong Cardiovascular Institute, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences","place":["Guangzhou, China"]},{"name":"Department of Anesthesiology, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Southern Medical University","place":["Guangzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fang","family":"Ye","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology, First Affiliated Hospital, Sun Yat-sen University","place":["Guangzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,9,28]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"91","DOI":"10.31083\/j.jin2103091","article-title":"Evaluation methods of a rat sciatic nerve crush injury model","volume":"21","author":"An","year":"2022","journal-title":"J. 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