{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T11:47:11Z","timestamp":1792237631325,"version":"4.4.0"},"reference-count":63,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2019,8,21]],"date-time":"2019-08-21T00:00:00Z","timestamp":1566345600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Neurol."],"DOI":"10.3389\/fneur.2019.00879","type":"journal-article","created":{"date-parts":[[2019,8,21]],"date-time":"2019-08-21T10:02:26Z","timestamp":1566381746000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":73,"title":["Comparison of Single Phase and Biphasic Extraction Protocols for Lipidomic Studies Using Human Plasma"],"prefix":"10.3389","volume":"10","author":[{"given":"Matthew Wai Kin","family":"Wong","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nady","family":"Braidy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Russell","family":"Pickford","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Perminder Singh","family":"Sachdev","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne","family":"Poljak","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2019,8,21]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"39571","DOI":"10.1038\/srep39571","article-title":"Mass spectrometry imaging of biomarker lipids for phagocytosis and signalling during focal cerebral ischaemia","volume":"6","author":"Nielsen","year":"2016","journal-title":"Sci Rep."},{"key":"B2","doi-asserted-by":"publisher","first-page":"219","DOI":"10.1038\/nri.2018.9","article-title":"Regulation of T cell signalling by membrane lipids","volume":"18","author":"Wu","year":"2018","journal-title":"Nat Rev Immunol."},{"key":"B3","first-page":"43","article-title":"The mitochondrial battlefield and membrane lipids during cell death signalling","volume":"52","author":"Degli Esposti","year":"2003","journal-title":"Ital J Biochem."},{"key":"B4","doi-asserted-by":"publisher","first-page":"10056","DOI":"10.1038\/ncomms10056","article-title":"Exclusive photorelease of signalling lipids at the plasma membrane","volume":"6","author":"Nadler","year":"2015","journal-title":"Nat Commun."},{"key":"B5","first-page":"73","article-title":"Biosynthesis of the myelin sheath","volume-title":"Lipids, Malnutrition and The Developing Brain.","author":"Davison","year":"1971"},{"key":"B6","doi-asserted-by":"publisher","first-page":"859","DOI":"10.1111\/j.1471-4159.1976.tb05147.x","article-title":"Effect of exogenous lipids on activites on the rat brain cholesterol ester hydrolase localized in the myelin sheath","volume":"27","author":"Igarashi","year":"1976","journal-title":"J Neurochem."},{"key":"B7","doi-asserted-by":"publisher","first-page":"225","DOI":"10.1016\/S0197-0186(96)00020-4","article-title":"Bioactive lipids in excitatory neurotransmission and neuronal plasticity","volume":"30","author":"Bazan","year":"1997","journal-title":"Neurochem Int."},{"key":"B8","doi-asserted-by":"publisher","first-page":"817","DOI":"10.1111\/j.1600-051X.2012.01920.x","article-title":"Serum lipids modify periodontal infection - C-reactive protein association","volume":"39","author":"Haro","year":"2012","journal-title":"J Clin Periodontol."},{"key":"B9","doi-asserted-by":"publisher","first-page":"485","DOI":"10.1007\/s10792-013-9728-4","article-title":"Association of dehydroepiandrosterone sulfate, serum lipids, C-reactive protein and body mass index with age-related macular degeneration","volume":"33","author":"Ulas","year":"2013","journal-title":"Int Ophthalmol."},{"key":"B10","doi-asserted-by":"publisher","first-page":"S5","DOI":"10.1002\/mnfr.201090016","article-title":"Lipids in health and disease","volume":"54","author":"Schneider","year":"2010","journal-title":"Mol Nutr Food Res."},{"key":"B11","doi-asserted-by":"publisher","first-page":"1442","DOI":"10.1016\/j.bbadis.2012.05.008","article-title":"Functions of plasmalogen lipids in health and disease","volume":"1822","author":"Braverman","year":"2012","journal-title":"Biochim Biophys Acta."},{"key":"B12","doi-asserted-by":"publisher","first-page":"47","DOI":"10.1038\/510047a","article-title":"Lipids in health and disease","volume":"510","author":"Finkelstein","year":"2014","journal-title":"Nature."},{"key":"B13","doi-asserted-by":"publisher","first-page":"336","DOI":"10.1016\/j.jacc.2017.11.031","article-title":"Imaging the intersection of oxidative stress, lipids, and inflammation: progress toward personalized care of atherosclerosis","volume":"71","author":"Tawakol","year":"2018","journal-title":"J Am Coll Cardiol."},{"key":"B14","doi-asserted-by":"publisher","first-page":"1229","DOI":"10.1093\/rheumatology\/keq037","article-title":"Inflammation, oxidative stress and lipids: the risk triad for atherosclerosis in gout","volume":"49","author":"Krishnan","year":"2010","journal-title":"Rheumatology."},{"key":"B15","doi-asserted-by":"publisher","first-page":"8","DOI":"10.1016\/j.prostaglandins.2016.12.002","article-title":"Recent advances in lipidomics: analytical and clinical perspectives","author":"Sethi","year":"2017","journal-title":"Prostaglandins Other Lipid Mediat."},{"key":"B16","doi-asserted-by":"publisher","first-page":"374","DOI":"10.1021\/acs.analchem.7b04836","article-title":"Analytical challenges and recent advances in mass spectrometry based lipidomics","volume":"90","author":"Rustam","year":"2018","journal-title":"Anal Chem."},{"key":"B17","doi-asserted-by":"publisher","first-page":"37","DOI":"10.1016\/j.jprot.2014.01.014","article-title":"Combination of metabolomic and phospholipid-profiling approaches for the study of Alzheimer's disease","volume":"104","author":"Gonzalez-Dominguez","year":"2014","journal-title":"J Proteomics."},{"key":"B18","doi-asserted-by":"publisher","first-page":"810","DOI":"10.1016\/j.jalz.2017.01.008","article-title":"Dysregulation of lipids in Alzheimer's disease and their role as potential biomarkers","volume":"13","author":"Wong","year":"2017","journal-title":"Alzheimer Demen."},{"key":"B19","doi-asserted-by":"publisher","first-page":"104","DOI":"10.3389\/fneur.2018.00104","article-title":"Lipidomics analysis of behavioral variant frontotemporal dementia: a scope for biomarker development","volume":"9","author":"Kim","year":"2018","journal-title":"Front Neurol."},{"key":"B20","doi-asserted-by":"publisher","first-page":"336","DOI":"10.1016\/j.neubiorev.2016.06.002","article-title":"Lipids in psychiatric disorders and preventive medicine","volume":"76","author":"Schneider","year":"2017","journal-title":"Neurosci Biobehav Rev."},{"key":"B21","doi-asserted-by":"publisher","first-page":"e1176","DOI":"10.1038\/tp.2017.152","article-title":"Serum fatty acid patterns in patients with schizophrenia: a targeted metabonomics study","volume":"7","author":"Yang","year":"2017","journal-title":"Translational Psychiatry."},{"key":"B22","doi-asserted-by":"publisher","first-page":"1812","DOI":"10.1194\/jlr.M034330","article-title":"A comparison of five lipid extraction solvent systems for lipidomic studies of human LDL","volume":"54","author":"Reis","year":"2013","journal-title":"J Lipid Res."},{"key":"B23","doi-asserted-by":"crossref","first-page":"497","DOI":"10.1016\/S0021-9258(18)64849-5","article-title":"A simple method for the isolation and purification of total lipides from animal tissues","volume":"226","author":"Folch","year":"1957","journal-title":"J Biol Chem."},{"key":"B24","doi-asserted-by":"publisher","first-page":"1137","DOI":"10.1194\/jlr.D700041-JLR200","article-title":"Lipid extraction by methyl-tert-butyl ether for high-throughput lipidomics","volume":"49","author":"Matyash","year":"2008","journal-title":"J Lipid Res."},{"key":"B25","doi-asserted-by":"publisher","first-page":"389","DOI":"10.3390\/metabo5020389","article-title":"An efficient single phase method for the extraction of plasma lipids","volume":"5","author":"Alshehry","year":"2015","journal-title":"Metabolites."},{"key":"B26","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/978-94-007-7864-1_90-1","article-title":"Liquid extraction: single-phase butanol\/methanol extraction","volume-title":"Encyclopedia of Lipidomics","author":"Alshehry","year":"2016"},{"key":"B27","doi-asserted-by":"publisher","first-page":"309","DOI":"10.1161\/HYPERTENSIONAHA.115.06501","article-title":"Prediction of cardiovascular disease risk by cardiac biomarkers in 2 United Kingdom cohort studies: does utility depend on risk thresholds for treatment?","volume":"67","author":"Welsh","year":"2016","journal-title":"Hypertension."},{"key":"B28","doi-asserted-by":"publisher","first-page":"351","DOI":"10.1016\/j.aca.2018.08.004","article-title":"Optimization of folch, bligh-dyer, and matyash sample-to-extraction solvent ratios for human plasma-based lipidomics studies","volume":"1037","author":"Ulmer","year":"2018","journal-title":"Anal Chim Acta."},{"key":"B29","doi-asserted-by":"publisher","first-page":"693","DOI":"10.1002\/mas.21492","article-title":"Selection of internal standards for accurate quantification of complex lipid species in biological extracts by electrospray ionization mass spectrometry-What, how and why?","volume":"36","author":"Wang","year":"2016","journal-title":"Mass Spectrom Rev."},{"key":"B30","doi-asserted-by":"publisher","first-page":"1248","DOI":"10.1017\/S1041610210001067","article-title":"The Sydney memory and ageing study (MAS): methodology and baseline medical and neuropsychiatric characteristics of an elderly epidemiological non-demented cohort of Australians aged 70\u201390 years","volume":"22","author":"Sachdev","year":"2010","journal-title":"Int Psychogeriatr."},{"key":"B31","doi-asserted-by":"publisher","first-page":"711","DOI":"10.3109\/09540261.2013.860890","article-title":"Sydney memory and ageing study: an epidemiological cohort study of brain ageing and dementia","volume":"25","author":"Tsang","year":"2013","journal-title":"Int Rev Psychiatry."},{"key":"B32","doi-asserted-by":"publisher","first-page":"240","DOI":"10.1111\/j.1365-2796.2004.01380.x","article-title":"Mild cognitive impairment\u2013beyond controversies, towards a consensus: report of the international working group on mild cognitive impairment","volume":"256","author":"Winblad","year":"2004","journal-title":"J Intern Med."},{"key":"B33","doi-asserted-by":"publisher","first-page":"1049","DOI":"10.1093\/ageing\/afv139","article-title":"Cognition and mortality in older people: the Sydney memory and ageing study","volume":"44","author":"Connors","year":"2015","journal-title":"Age Ageing."},{"key":"B34","doi-asserted-by":"publisher","first-page":"549","DOI":"10.1016\/j.jalz.2014.08.099","article-title":"Guidelines for the standardization of preanalytic variables for blood-based biomarker studies in Alzheimer's disease research","volume":"11","author":"O'Bryant","year":"2015","journal-title":"Alzheimers Dement."},{"key":"B35","doi-asserted-by":"publisher","first-page":"112","DOI":"10.1111\/acel.12275","article-title":"Factors affecting longitudinal trajectories of plasma sphingomyelins: the Baltimore Longitudinal Study of Aging","volume":"14","author":"Mielke","year":"2015","journal-title":"Aging Cell."},{"key":"B36","doi-asserted-by":"publisher","first-page":"2001","DOI":"10.1194\/jlr.S087163","article-title":"MS-based lipidomics of human blood plasma: a community-initiated position paper to develop accepted guidelines","volume":"59","author":"Burla","year":"2018","journal-title":"J Lipid Res."},{"key":"B37","doi-asserted-by":"publisher","first-page":"1637","DOI":"10.1161\/CIRCULATIONAHA.116.023233","article-title":"Plasma lipidomic profiles improve on traditional risk factors for the prediction of cardiovascular events in type 2 diabetes mellitus","volume":"134","author":"Alshehry","year":"2016","journal-title":"Circulation."},{"key":"B38","doi-asserted-by":"publisher","first-page":"1168","DOI":"10.1046\/j.1471-4159.2001.00332.x","article-title":"Plasmalogen deficiency in early Alzheimer's disease subjects and in animal models: molecular characterization using electrospray ionization mass spectrometry","volume":"77","author":"Han","year":"2001","journal-title":"J Neurochem."},{"key":"B39","doi-asserted-by":"publisher","first-page":"1310","DOI":"10.3390\/ijms14011310","article-title":"Phospholipids and Alzheimer's disease: alterations, mechanisms and potential biomarkers","volume":"14","author":"Kosicek","year":"2013","journal-title":"Int J Mol Sci."},{"key":"B40","doi-asserted-by":"publisher","first-page":"145","DOI":"10.1016\/j.chroma.2016.06.055","article-title":"Optimization of a single phase method for lipid extraction from milk","volume":"1458","author":"Liu","year":"2016","journal-title":"J Chromatogr A."},{"key":"B41","doi-asserted-by":"publisher","first-page":"1690","DOI":"10.1194\/jlr.D023036","article-title":"The BUME method: a novel automated chloroform-free 96-well total lipid extraction method for blood plasma","volume":"53","author":"Lofgren","year":"2012","journal-title":"J Lipid Res."},{"key":"B42","doi-asserted-by":"publisher","first-page":"27688","DOI":"10.1038\/srep27688","article-title":"The BUME method: a new rapid and simple chloroform-free method for total lipid extraction of animal tissue","volume":"6","author":"Lofgren","year":"2016","journal-title":"Sci Rep."},{"key":"B43","doi-asserted-by":"publisher","first-page":"7937","DOI":"10.1007\/s00216-014-8255-0","article-title":"LC\/MS lipid profiling from human serum: a new method for global lipid extraction","volume":"406","author":"Pellegrino","year":"2014","journal-title":"Anal Bioanal Chem."},{"key":"B44","doi-asserted-by":"publisher","first-page":"5859","DOI":"10.1007\/s00216-018-1200-x","article-title":"One- vs two-phase extraction: re-evaluation of sample preparation procedures for untargeted lipidomics in plasma samples","volume":"410","author":"Gil","year":"2018","journal-title":"Anal Bioanal Chem."},{"key":"B45","doi-asserted-by":"publisher","first-page":"653","DOI":"10.1038\/s41467-017-00413-x","article-title":"Establishing multiple omics baselines for three Southeast Asian populations in the Singapore integrative omics study","volume":"8","author":"Saw","year":"2017","journal-title":"Nat Commun."},{"key":"B46","doi-asserted-by":"publisher","first-page":"428","DOI":"10.1111\/joim.12166","article-title":"Transfer of omega-3 fatty acids across the blood-brain barrier after dietary supplementation with a docosahexaenoic acid-rich omega-3 fatty acid preparation in patients with Alzheimer's disease: the OmegAD study","volume":"275","author":"Freund Levi","year":"2014","journal-title":"J Intern Med."},{"key":"B47","doi-asserted-by":"publisher","first-page":"503","DOI":"10.1038\/nature13241","article-title":"Mfsd2a is a transporter for the essential omega-3 fatty acid docosahexaenoic acid","volume":"509","author":"Nguyen","year":"2014","journal-title":"Nature."},{"key":"B48","doi-asserted-by":"publisher","first-page":"38","DOI":"10.1186\/1750-1326-8-38","article-title":"Blood-brain-barriers in aging and in Alzheimer's disease","volume":"8","author":"Marques","year":"2013","journal-title":"Mol Neurodegener."},{"key":"B49","doi-asserted-by":"publisher","first-page":"1759091417719201","DOI":"10.1177\/1759091417719201","article-title":"Peripheral inflammation, apolipoprotein E4, and amyloid-beta interact to induce cognitive and cerebrovascular dysfunction","volume":"9","author":"Marottoli","year":"2017","journal-title":"ASN Neuro."},{"key":"B50","doi-asserted-by":"publisher","first-page":"e63644","DOI":"10.1371\/journal.pone.0063644","article-title":"identification of altered metabolic pathways in plasma and CSF in mild cognitive impairment and Alzheimer's disease using metabolomics","volume":"8","author":"Trushina","year":"2013","journal-title":"PLoS ONE."},{"key":"B51","doi-asserted-by":"publisher","first-page":"6777","DOI":"10.1038\/srep06777","article-title":"Correlations of amyloid-\u03b2 concentrations between CSF and plasma in acute Alzheimer mouse model","volume":"4","author":"Cho","year":"2014","journal-title":"Sci Reports."},{"key":"B52","doi-asserted-by":"publisher","first-page":"887","DOI":"10.3233\/JAD-150948","article-title":"Plasma phospholipid and sphingolipid alterations in presenilin1 mutation carriers: a pilot study","volume":"50","author":"Chatterjee","year":"2016","journal-title":"J Alzheimers Dis."},{"key":"B53","doi-asserted-by":"publisher","first-page":"140","DOI":"10.1016\/j.jalz.2016.08.003","article-title":"Association of blood lipids with Alzheimer's disease: a comprehensive lipidomics analysis","volume":"13","author":"Proitsi","year":"2016","journal-title":"Alzheimers Demen."},{"key":"B54","doi-asserted-by":"publisher","first-page":"e34078","DOI":"10.1371\/journal.pone.0034078","article-title":"Plasma apolipoprotein levels are associated with cognitive status and decline in a community cohort of older individuals","volume":"7","author":"Song","year":"2012","journal-title":"PLoS ONE."},{"key":"B55","doi-asserted-by":"publisher","first-page":"49","DOI":"10.1016\/j.neurobiolaging.2017.02.017","article-title":"Plasma apolipoproteins and physical and cognitive health in very old individuals","volume":"55","author":"Muenchhoff","year":"2017","journal-title":"Neurobiol Aging."},{"key":"B56","doi-asserted-by":"publisher","first-page":"10141","DOI":"10.1523\/JNEUROSCI.1054-16.2016","article-title":"Genetic restoration of plasma ApoE improves cognition and partially restores synaptic defects in ApoE-deficient mice","volume":"36","author":"Lane-Donovan","year":"2016","journal-title":"J Neurosci."},{"key":"B57","doi-asserted-by":"publisher","first-page":"633","DOI":"10.1212\/WNL.0b013e318264e380","article-title":"Serum ceramides increase the risk of Alzheimer disease: the women's health and aging study II","volume":"79","author":"Mielke","year":"2012","journal-title":"Neurology."},{"key":"B58","doi-asserted-by":"publisher","first-page":"415","DOI":"10.1038\/nm.3466","article-title":"Plasma phospholipids identify antecedent memory impairment in older adults","volume":"20","author":"Mapstone","year":"2014","journal-title":"Nat Med."},{"key":"B59","doi-asserted-by":"publisher","first-page":"237","DOI":"10.3389\/fneur.2015.00237","article-title":"Plasma 24-metabolite panel predicts preclinical transition to clinical stages of Alzheimer's disease","volume":"6","author":"Fiandaca","year":"2015","journal-title":"Front Neurol."},{"key":"B60","doi-asserted-by":"publisher","first-page":"771","DOI":"10.1023\/A:1011603916962","article-title":"Brain membrane phospholipid alterations in Alzheimer's disease","volume":"26","author":"Pettegrew","year":"2001","journal-title":"Neurochem Res."},{"key":"B61","doi-asserted-by":"publisher","first-page":"19139","DOI":"10.1038\/srep19139","article-title":"Discovering and validating between-subject variations in plasma lipids in healthy subjects","volume":"6","author":"Begum","year":"2016","journal-title":"Sci Rep."},{"key":"B62","doi-asserted-by":"publisher","first-page":"25","DOI":"10.1016\/j.clinms.2017.11.002","article-title":"Lipidomic profiling of plasma in a healthy Singaporean population to identify ethnic specific differences in lipid levels and associations with disease risk factors","volume":"6","author":"Begum","year":"2017","journal-title":"Clin Mass Spectrom."},{"key":"B63","doi-asserted-by":"publisher","first-page":"e0214141","DOI":"10.1371\/journal.pone.0214141","article-title":"Plasma lipidome variation during the second half of the human lifespan is associated with age and sex but minimally with BMI","volume":"14","author":"Wong","year":"2019","journal-title":"PLoS ONE."}],"container-title":["Frontiers in Neurology"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/article\/10.3389\/fneur.2019.00879\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,1,17]],"date-time":"2021-01-17T04:14:34Z","timestamp":1610856874000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/article\/10.3389\/fneur.2019.00879\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,8,21]]},"references-count":63,"alternative-id":["10.3389\/fneur.2019.00879"],"URL":"https:\/\/doi.org\/10.3389\/fneur.2019.00879","relation":{},"ISSN":["1664-2295"],"issn-type":[{"value":"1664-2295","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,8,21]]},"article-number":"879"}}