{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,15]],"date-time":"2025-12-15T14:18:17Z","timestamp":1765808297194,"version":"3.41.2"},"reference-count":17,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2023,12,14]],"date-time":"2023-12-14T00:00:00Z","timestamp":1702512000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Neurol."],"abstract":"<jats:p>Peripheral neuropathy with antibodies to myelin-associated glycoprotein (MAG) is an autoimmune demyelinating disorder of the peripheral nervous system caused by pathogenic IgM recognizing the human natural killer-1 glycoepitope expressed on MAG. This study aimed to analyze the performance of a new indirect immunofluorescence cell-based assay (CBA, EUROIMMUN) for the detection of anti-MAG IgM. Antibody reactivity was determined in sera from 95 patients with clinical and neurophysiological evidence of anti-MAG-associated neuropathy and in control samples from 55 patients with other forms of peripheral neuropathy. Compared to the results of the gold standard method (ELISA, B\u00fchlmann) and using samples at a dilution of 1:100, the CBA had a sensitivity of 98.9% and a specificity of 100% (PPV 100%, NPV 98.2%). In conclusion, the CBA allows the detection of antibodies to MAG using an easy and standardized technique, and it presents a sensitive and specific alternative to the more time-consuming ELISA. Larger studies are needed to address anti-MAG titer monitoring in parallel with clinical activity.<\/jats:p>","DOI":"10.3389\/fneur.2023.1289810","type":"journal-article","created":{"date-parts":[[2023,12,14]],"date-time":"2023-12-14T17:25:55Z","timestamp":1702574755000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":3,"title":["A novel cell-based immunofluorescence assay for the detection of autoantibodies to myelin-associated glycoprotein"],"prefix":"10.3389","volume":"14","author":[{"given":"Sara","family":"Mariotto","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Piera","family":"de Gaspari","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dominik","family":"J\u00e4ger","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stefanie","family":"Hahn","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cindy","family":"Forni","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sandra","family":"Saschenbrecker","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Erik","family":"Lattwein","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alessandro","family":"Dinoto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sergio","family":"Ferrari","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2023,12,14]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"7139","DOI":"10.1073\/pnas.78.11.7139","article-title":"Plasma cell dyscrasia and peripheral neuropathy: identification of the myelin antigens that react with human paraproteins","volume":"78","author":"Latov","year":"1981","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref2","doi-asserted-by":"publisher","first-page":"38","DOI":"10.1002\/(SICI)1097-4598(199701)20:1<38::AID-MUS5>3.0.CO;2-I","article-title":"Physiological tremor analysis of patients with anti-myelin-associated glycoprotein associated neuropathy and tremor","volume":"20","author":"Pedersen","year":"1997","journal-title":"Muscle Nerve"},{"key":"ref3","doi-asserted-by":"publisher","first-page":"789","DOI":"10.1093\/brain\/119.3.789","article-title":"Tremor associated with benign IgM paraproteinaemic neuropathy","volume":"119","author":"Bain","year":"1996","journal-title":"Brain"},{"key":"ref4","doi-asserted-by":"publisher","first-page":"383","DOI":"10.1007\/BF00319857","article-title":"The clinical spectrum of peripheral neuropathies associated with benign monoclonal IgM, IgG and IgA paraproteinaemia. 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