{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T04:12:30Z","timestamp":1792037550504,"version":"4.4.0"},"reference-count":38,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2025,9,23]],"date-time":"2025-09-23T00:00:00Z","timestamp":1758585600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Neurol."],"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Stroke-induced upper limb dysfunction significantly impacts rehabilitation outcomes. Transcutaneous Electrical Nerve Stimulation (TENS) shows promise, but the optimal frequency for motor recovery remains unclear. This study investigates the comparative effects of low-frequency (2 Hz) and high-frequency (100 Hz) TENS in post-stroke upper limb rehabilitation. This article describes the design and conduct of this trial.<\/jats:p><\/jats:sec><jats:sec><jats:title>Method\/design<\/jats:title><jats:p>An assessor-blinded, single-center randomized controlled trial with partial participant blinding (active vs. placebo) will recruit 156 participants aged 40\u201380 years, 10 days to 2 months post-stroke, with mild-to-moderate upper-limb impairment (Brunnstrom III\u2013V). Participants will be randomly assigned (1:1:1:1) to 2 Hz TENS, 100 Hz TENS, placebo TENS, or a no-TENS usual-care control for 8 weeks (3 sessions\/week, 30 minutes\/session). The primary outcome is the Fugl\u2013Meyer Assessment of the Upper Extremity (FMA-UE). Secondary outcomes include Manual Muscle Testing (MMT), the Modified Ashworth Scale, electroencephalography (EEG) metrics, the Lindmark Motor Score (hand subscale), and the Barthel Index, assessed at baseline, weeks 4 and 8, and at 1 and 3 months post-intervention.<\/jats:p><\/jats:sec><jats:sec><jats:title>Discussion<\/jats:title><jats:p>This trial is, to our knowledge, among the first randomized head-to-head comparisons of low- vs. high-frequency TENS for post-stroke upper-limb rehabilitation. Its findings are expected to clarify frequency-dependent effects, narrow the plausible range of effective parameters, and provide evidence to inform clinical guidelines and future rehabilitation strategies.<\/jats:p><\/jats:sec>","DOI":"10.3389\/fneur.2025.1641781","type":"journal-article","created":{"date-parts":[[2025,9,23]],"date-time":"2025-09-23T04:13:11Z","timestamp":1758600791000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":2,"title":["Comparative effects of 2 Hz vs. 100 Hz transcutaneous electrical nerve stimulation on upper limb motor function post-stroke: design and rationale for a randomized trial"],"prefix":"10.3389","volume":"16","author":[{"given":"Chengning","family":"Song","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huanxin","family":"Xie","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bo","family":"Lei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nana","family":"Feng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhixian","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanxian","family":"Zou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2025,9,23]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"2095","DOI":"10.1016\/S0140-6736(12)61728-0","article-title":"Global and regional mortality from 235 causes of death for 20 age groups in 1990 and 2010: a systematic analysis for the Global Burden of Disease Study 2010","volume":"380","author":"Lozano","year":"2012","journal-title":"Lancet."},{"key":"B2","doi-asserted-by":"publisher","first-page":"2197","DOI":"10.1016\/S0140-6736(12)61689-4","article-title":"Disability-adjusted life years (DALYs) for 291 diseases and injuries in 21 regions, 1990-2010: a systematic analysis for the Global Burden of Disease Study 2010","volume":"380","author":"Murray","year":"2012","journal-title":"Lancet."},{"key":"B3","doi-asserted-by":"publisher","first-page":"745","DOI":"10.1161\/STROKEAHA.109.572065","article-title":"Harmeling-van der Wel BC, Kwakkel G. 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