{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,10]],"date-time":"2026-07-10T05:13:55Z","timestamp":1783660435220,"version":"3.55.0"},"reference-count":103,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,7,10]],"date-time":"2026-07-10T00:00:00Z","timestamp":1783641600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Neurol."],"abstract":"<jats:p>\n                    Epilepsy is a chronic neurological disorder that is strongly influenced by oxidative stress and neuronal death, two major pathophysiological factors. According to various recent studies,\n                    <jats:italic>Ganoderma lucidum<\/jats:italic>\n                    extract (GLE) may have antioxidant properties. However, there is little evidence to support its effectiveness in treating epilepsy. Here, we examined the neuroprotective qualities of GLE (300\u202fmg\/kg) against seizures induced by pentylenetetrazol (35\u202fmg\/kg). Four groups of eight male Wistar rats per group were randomly assigned: control, Pentylenetetrazol (PTZ), GLE-300 and Diazepam. The animals received an intraperitoneal dose of PTZ (35\u202fmg\/kg) every 48\u202fh (11 injections in total) for 21\u202fdays to create an epilepsy model. The Racine five-point score was used to assess seizure severity. Anxious behavior, locomotor and exploratory activity were assessed using the open field test (OFT) and the elevated plus maze test (EPM), while the forced swim test (FST) was used to assess depressive behavior. Markers of oxidative stress, including malondialdehyde (MDA), nitric oxide (NO), and the enzymatic activities of superoxide dismutase (SOD) and catalase, were measured using the 2-thiobarbituric acid method (TBA), Griess reagent, nitro blue tetrazolium (NBT) reduction, and hydrogen peroxide decomposition. We performed a histological study of the prefrontal cortex (PFC) to quantify neuronal loss in the cortical layers using Nissl staining. All in all, our results showed that GLE reduces the severity of convulsive seizures during the induction of epilepsy (\n                    <jats:italic>p<\/jats:italic>\n                    &amp;lt;\u202f0.001) and alleviates anxious (\n                    <jats:italic>p<\/jats:italic>\n                    =\u202f0.005) and depressive (\n                    <jats:italic>p<\/jats:italic>\n                    =\u202f0.008) behaviors while reducing neuronal damage. As well as significantly reduce NO and MDA levels (\n                    <jats:italic>p<\/jats:italic>\n                    &amp;lt;\u202f0.001), and restore catalase (\n                    <jats:italic>p<\/jats:italic>\n                    =\u202f0.005) and SOD (\n                    <jats:italic>p<\/jats:italic>\n                    =\u202f0.010) enzyme activities in biochemical evaluation. Histologically, the GLE reduces neurodegeneration in the PFC due to its antioxidant and neuroprotective properties (\n                    <jats:italic>p<\/jats:italic>\n                    =\u202f0.003). In conclusion, the experimental results indicate that GLE significantly attenuates the behavioral, biochemical, and histological alterations induced by PTZ, thus supporting its neurotherapeutic potential in the PTZ-Kindling model of epilepsy.\n                  <\/jats:p>","DOI":"10.3389\/fneur.2026.1752481","type":"journal-article","created":{"date-parts":[[2026,7,10]],"date-time":"2026-07-10T04:25:50Z","timestamp":1783657550000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Potential neuroprotective and anticonvulsant effects of Ganoderma lucidum ethanol extract and its impact on affective comorbidities associated with pentylenetetrazol kindling model of epilepsy: behavioral, biochemical and histological studies"],"prefix":"10.3389","volume":"17","author":[{"given":"Itto Rahou","family":"Abdessamad","sequence":"first","affiliation":[{"name":"Laboratory of Biology and Health, Neurosciences, Neuroimmunology and Behaviour Unit, Faculty of Science, Ibn Tofail University","place":["Kenitra, Morocco"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bikjdaouene","family":"Leila","sequence":"additional","affiliation":[{"name":"Laboratory of Biology and Health, Neurosciences, Neuroimmunology and Behaviour Unit, Faculty of Science, Ibn Tofail University","place":["Kenitra, Morocco"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bahbiti","family":"Youssef","sequence":"additional","affiliation":[{"name":"Laboratory of Biology and Health, Neurosciences, Neuroimmunology and Behaviour Unit, Faculty of Science, Ibn Tofail University","place":["Kenitra, Morocco"]},{"name":"Higher Institute of Nursing and Health Technology of Tanger (ISPITS)","place":["Tangier, Morocco"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sqalli Houssaini","family":"Youssef","sequence":"additional","affiliation":[{"name":"Laboratory of Biology and Health, Neurosciences, Neuroimmunology and Behaviour Unit, Faculty of Science, Ibn Tofail University","place":["Kenitra, Morocco"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"El Mekhlouf","family":"Youssef","sequence":"additional","affiliation":[{"name":"Laboratory of Biology and Health, Neurosciences, Neuroimmunology and Behaviour Unit, Faculty of Science, Ibn Tofail University","place":["Kenitra, Morocco"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Azeroil","family":"Fatima","sequence":"additional","affiliation":[{"name":"Laboratory of Biology and Health, Neurosciences, Neuroimmunology and Behaviour Unit, Faculty of Science, Ibn Tofail University","place":["Kenitra, Morocco"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Doumar","family":"Hanan","sequence":"additional","affiliation":[{"name":"Laboratory of Biology and Health, Neurosciences, Neuroimmunology and Behaviour Unit, Faculty of Science, Ibn Tofail University","place":["Kenitra, Morocco"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dimaoui","family":"Amal","sequence":"additional","affiliation":[{"name":"Laboratory of Biology and Health, Neurosciences, Neuroimmunology and Behaviour Unit, Faculty of Science, Ibn Tofail University","place":["Kenitra, Morocco"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"El-Hessni","family":"Aboubaker","sequence":"additional","affiliation":[{"name":"Laboratory of Biology and Health, Neurosciences, Neuroimmunology and Behaviour Unit, Faculty of Science, Ibn Tofail University","place":["Kenitra, Morocco"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mesfioui","family":"Abdelhalem","sequence":"additional","affiliation":[{"name":"Laboratory of Biology and Health, Neurosciences, Neuroimmunology and Behaviour Unit, Faculty of Science, Ibn Tofail University","place":["Kenitra, Morocco"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,7,10]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"604158","DOI":"10.3389\/fnmol.2020.604158","article-title":"Neuroproteomics in epilepsy: what do we know so far?","volume":"13","author":"Do Canto","year":"2021","journal-title":"Front Mol Neurosci"},{"key":"ref2","doi-asserted-by":"publisher","first-page":"1225","DOI":"10.1111\/j.1528-1167.2006.00595.x","article-title":"Epilepsy Care in the World: results of an ILAE\/IBE\/WHO global campaign against epilepsy survey","volume":"47","author":"Dua","year":"2006","journal-title":"Epilepsia"},{"key":"ref3","doi-asserted-by":"publisher","first-page":"1753","DOI":"10.1016\/j.ejmech.2010.01.007","article-title":"Derivatives of benzimidazole pharmacophore: synthesis, anticonvulsant, antidiabetic and DNA cleavage studies","volume":"45","author":"Shingalapur","year":"2010","journal-title":"Eur J Med 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