{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,10]],"date-time":"2026-11-10T07:00:46Z","timestamp":1794294046529,"version":"4.9.0"},"reference-count":40,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,4,13]],"date-time":"2026-04-13T00:00:00Z","timestamp":1776038400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Neurol."],"abstract":"<jats:p>\n                    GBS (Guillain\u2013Barr\u00e9 syndrome) is an acute immune-mediated peripheral neuropathy whose pathological mechanisms remain incompletely understood. Our study aimed to explore the intrinsic link and potential mechanisms between circulating dyslipidemia and immune metabolic remodeling of monocytes in GBS. Clinical data from 163 patients diagnosed with classic GBS and 169 healthy controls were analyzed to examine the correlations between their lipid profiles and immune cell features. Leveraging our team\u2019s previously published single-cell RNA sequencing (scRNA-seq) dataset for GBS, we characterized the subpopulations, functional features, and differentiation trajectories of peripheral blood monocytes. Furthermore, through transcriptional regulatory network analysis, we identified key transcription factors governing these processes. Compared to healthy controls, GBS patients displayed a distinct dyslipidemia characterized by elevated triglycerides (TG), very low-density lipoprotein (VLDL), and residual cholesterol (RC), but decreased high-density lipoprotein (HDL) and apolipoprotein A1 (APOA1). This lipid profile coincided with higher monocyte counts in peripheral blood, suggesting a synergistic relationship. The scRNA-seq analysis results indicate that the composition of peripheral blood monocyte subsets in GBS patients undergoes fundamental remodeling. This is characterized by a significant expansion of CD14\n                    <jats:sup>+<\/jats:sup>\n                    CD163\n                    <jats:sup>+<\/jats:sup>\n                    monocytes lipid metabolism-active monocytes and pro-inflammatory CD14\n                    <jats:sup>+<\/jats:sup>\n                    CD169\n                    <jats:sup>+<\/jats:sup>\n                    monocytes, while the proportion of classical monocytes sharply decreases. Pseudo-time trajectory analysis revealed a differentiation pathway from classical monocytes to CD14\n                    <jats:sup>+<\/jats:sup>\n                    CD163\n                    <jats:sup>+<\/jats:sup>\n                    monocytes via CD14\n                    <jats:sup>+<\/jats:sup>\n                    CD169\n                    <jats:sup>+<\/jats:sup>\n                    and intermediate monocytes. This pathway was characterized by an initial increase followed by a decrease in pro-inflammatory activity, coupled with a progressive enhancement in lipid metabolism activity. Transcriptional network analysis identified CEBPB as a core transcription factor potentially associated with the phenotypic conversion of monocyte subsets, likely mediated by synergistic regulation of genes involved in lipid metabolism and cell differentiation. In summary, GBS is characterized by synergistic dyslipidemia and monocyte remodeling. The pathological signature involves lipid metabolic reprogramming in the CD14\n                    <jats:sup>+<\/jats:sup>\n                    CD163\n                    <jats:sup>+<\/jats:sup>\n                    monocytes and pro-inflammatory phenotypes in the CD14\n                    <jats:sup>+<\/jats:sup>\n                    CD169\n                    <jats:sup>+<\/jats:sup>\n                    monocytes. The transcription factor CEBPB is associated with this phenotypic conversion by regulating lipid metabolism and differentiation genes, revealing molecular targets for precise GBS diagnosis and therapy.\n                  <\/jats:p>","DOI":"10.3389\/fneur.2026.1797582","type":"journal-article","created":{"date-parts":[[2026,4,13]],"date-time":"2026-04-13T04:44:13Z","timestamp":1776055453000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":1,"title":["Dyslipidemia is associated with monocyte lipid metabolic reprogramming in Guillain\u2013Barr\u00e9 syndrome"],"prefix":"10.3389","volume":"17","author":[{"given":"Yuan","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Neurology, First Affiliated Hospital, Harbin Medical University","place":["Harbin, Heilongjiang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuanghong","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Neurology, First Affiliated Hospital, Harbin Medical University","place":["Harbin, Heilongjiang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feihong","family":"Jia","sequence":"additional","affiliation":[{"name":"Department of Neurology, First Affiliated Hospital, Harbin Medical University","place":["Harbin, Heilongjiang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meng","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Neurology, First Affiliated Hospital, Harbin Medical University","place":["Harbin, Heilongjiang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haining","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Neurology, First Affiliated Hospital, Harbin Medical University","place":["Harbin, Heilongjiang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jihe","family":"Song","sequence":"additional","affiliation":[{"name":"Department of Neurology, First Affiliated Hospital, Harbin Medical University","place":["Harbin, Heilongjiang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongping","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Neurology, First Affiliated Hospital, Harbin Medical University","place":["Harbin, Heilongjiang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Di","family":"Zhong","sequence":"additional","affiliation":[{"name":"Department of Neurology, First Affiliated Hospital, Harbin Medical University","place":["Harbin, Heilongjiang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,4,13]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"150","DOI":"10.1159\/000184748","article-title":"The epidemiology of Guillain-Barr\u00e9 syndrome worldwide","volume":"32","author":"McGrogan","year":"2009","journal-title":"Neuroepidemiology"},{"key":"ref2","doi-asserted-by":"publisher","first-page":"100302","DOI":"10.1016\/j.lanwpc.2021.100302","article-title":"Incidence of Guillain-Barr\u00e9 syndrome (GBS) in China: a national population-based study","volume":"18","author":"Zheng","year":"2022","journal-title":"Lancet Reg Health West Pac"},{"key":"ref3","doi-asserted-by":"publisher","first-page":"97","DOI":"10.1038\/s41572-024-00580-4","article-title":"Guillain\u2013Barr\u00e9 syndrome","volume":"10","author":"Leonhard","year":"2024","journal-title":"Nat Rev Dis Primers"},{"key":"ref4","doi-asserted-by":"publisher","first-page":"33","DOI":"10.1093\/brain\/awt285","article-title":"Diagnosis of Guillain-Barre syndrome and validation of Brighton criteria","volume":"137","author":"Fokke","year":"2013","journal-title":"Brain"},{"key":"ref5","doi-asserted-by":"publisher","first-page":"2329","DOI":"10.1016\/j.clinph.2004.05.009","article-title":"Neurophysiologic findings in early acute inflammatory demyelinating polyradiculoneuropathy","volume":"115","author":"Vucic","year":"2004","journal-title":"Clin Neurophysiol"},{"key":"ref6","doi-asserted-by":"publisher","first-page":"173","DOI":"10.1007\/978-1-4939-8552-4_7","article-title":"Anti-ganglioside antibodies in peripheral nerve pathology","volume":"1804","author":"Willison","year":"2018","journal-title":"Methods Mol Biol"},{"key":"ref7","doi-asserted-by":"publisher","first-page":"e200107","DOI":"10.1212\/NXI.0000000000200107","article-title":"High and persistent anti-GM1 antibody titers are associated with poor clinical recovery in Guillain-Barr\u00e9 syndrome","volume":"10","author":"Thomma","year":"2023","journal-title":"Neurol Neuroimmunol Neuroinflamm"},{"key":"ref8","doi-asserted-by":"publisher","first-page":"CD008630","DOI":"10.1002\/14651858.CD008630.pub5","article-title":"Pharmacological treatment other than corticosteroids, intravenous immunoglobulin and plasma exchange for Guillain-Barr\u00e9 syndrome","volume":"2020","author":"Doets","year":"2020","journal-title":"Cochrane Database Syst Rev"},{"key":"ref9","doi-asserted-by":"publisher","first-page":"2866","DOI":"10.1093\/brain\/awy232","article-title":"Regional variation of Guillain-Barr\u00e9 syndrome","volume":"141","author":"Doets","year":"2018","journal-title":"Brain"},{"key":"ref10","doi-asserted-by":"publisher","first-page":"626725","DOI":"10.3389\/fimmu.2020.626725","article-title":"Editorial: monocyte heterogeneity and function","volume":"11","author":"Ginhoux","year":"2021","journal-title":"Front 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inflammatory demyelinating polyneuropathy","volume":"13","author":"Li","year":"2023","journal-title":"Sci Rep"},{"key":"ref18","doi-asserted-by":"publisher","first-page":"4","DOI":"10.1186\/1471-2172-15-4","article-title":"CD14+CD16+ and CD14+CD163+ monocyte subpopulations in kidney allograft transplantation","volume":"15","author":"Sekerkova","year":"2014","journal-title":"BMC Immunol"},{"key":"ref19","doi-asserted-by":"publisher","first-page":"13","DOI":"10.1016\/j.molimm.2019.01.017","article-title":"Circulating CD14+CD163+CD209+ M2-like monocytes are associated with the severity of infection in Helicobacter pylori-positive patients","volume":"108","author":"Hou","year":"2019","journal-title":"Mol Immunol"},{"key":"ref20","doi-asserted-by":"publisher","first-page":"e0141817","DOI":"10.1371\/journal.pone.0141817","article-title":"A higher frequency of CD14+CD169+ monocytes\/macrophages in patients with colorectal 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Aggan","year":"2021","journal-title":"Biom J"},{"key":"ref38","doi-asserted-by":"publisher","first-page":"12032","DOI":"10.3390\/ijms241512032","article-title":"Impact of lipid metabolism on macrophage polarization: implications for inflammation and tumor immunity","volume":"24","author":"Vassiliou","year":"2023","journal-title":"Int J Mol Sci"},{"key":"ref39","doi-asserted-by":"publisher","first-page":"1321","DOI":"10.3390\/cells8111321","article-title":"Transcriptional regulation of autophagy genes via stage-specific activation of CEBPB and PPARG during Adipogenesis: a systematic study using public gene expression and transcription factor binding datasets","volume":"8","author":"Ahmed","year":"2019","journal-title":"Cells"},{"key":"ref40","doi-asserted-by":"publisher","first-page":"1011279","DOI":"10.3389\/fimmu.2022.1011279","article-title":"Central gene transcriptional regulatory networks shaping monocyte development in bone 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