{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T05:11:13Z","timestamp":1778562673881,"version":"3.51.4"},"reference-count":43,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T00:00:00Z","timestamp":1778544000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Neurol."],"abstract":"<jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>The objective of this study was to investigate the neuroprotective effects of long non-coding RNA colorectal neoplasia differentially expressed (CRNDE) on ischemic spinal cords.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and methods<\/jats:title>\n                    <jats:p>The binding relationship between CRNDE and microRNA-181a-5p was detected using dual luciferase assays. Spinal cord ischemia was induced in rats by cross clamping the descending aorta. CRNDE expression was induced by intrathecal injection of adeno-associated virus vectors containing CRNDE. The hind-limb motor function of the rats was then assessed over a period of 3 weeks following reperfusion. Lumbar spinal cords were harvested for histologic examinations. Expressions of CRNDE, microRNA-181a-5p and related proteins were measured by quantitative reverse transcription polymerase chain reaction and Western blot.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Luciferase assays demonstrated that CRNDE bound to microRNA-181a-5p, and Sirt1 was a direct target of microRNA-181a-5p. The transient ischemia induced a significant decrease of CRNDE expression accompanied by a robust increase of microRNA-181a-5p expression in spinal cords. Intrathecal injection of adeno-associated virus vectors containing CRNDE resulted in a significant enhancement of CRNDE expression and a repression of microRNA-181a-5p expression in spinal cords. Consequently, CRNDE overexpression was found to inhibit neuronal apoptosis, attenuate histologic damage, increase the number of surviving neurons, and improve the hind-limb motor function after spinal cord ischemia.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>CRNDE overexpression induces spinal cord protection against ischemia\u2013reperfusion injury, possibly via microRNA-181a-5p\/Sirt1 axis.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fneur.2026.1825718","type":"journal-article","created":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T04:29:43Z","timestamp":1778560183000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Overexpression of long noncoding RNA colorectal neoplasia differentially expressed protects spinal cords against ischemia by targeting microRNA-181a-5p\/Sirtuin-1 axis"],"prefix":"10.3389","volume":"17","author":[{"given":"Zhi","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Cardiac Surgery, First Affiliated Hospital, China Medical University","place":["Shenyang, China"]},{"name":"Department of Cardiac Surgery, The People\u2019s Hospital of Bozhou","place":["Anhui, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Cardiac Surgery, First Affiliated Hospital, China Medical University","place":["Shenyang, China"]},{"name":"State Key Laboratory of Cardiovascular Disease, Center of Vascular Surgery, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College","place":["Beijing, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rui","family":"Tang","sequence":"additional","affiliation":[{"name":"Department of Cardiac Surgery, First Affiliated Hospital, China Medical University","place":["Shenyang, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shilun","family":"Gao","sequence":"additional","affiliation":[{"name":"Department of Cardiac Surgery, First Affiliated Hospital, China Medical University","place":["Shenyang, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yubao","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Cardiac Surgery, First Affiliated Hospital, China Medical University","place":["Shenyang, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tianxiang","family":"Gu","sequence":"additional","affiliation":[{"name":"Department of Cardiac Surgery, First Affiliated Hospital, China Medical University","place":["Shenyang, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaojing","family":"Jiang","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology, First 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