{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T16:10:33Z","timestamp":1753891833367,"version":"3.41.2"},"reference-count":30,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2021,10,4]],"date-time":"2021-10-04T00:00:00Z","timestamp":1633305600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000053","name":"National Eye Institute","doi-asserted-by":"publisher","award":["1R01 EY028216-01A1"],"award-info":[{"award-number":["1R01 EY028216-01A1"]}],"id":[{"id":"10.13039\/100000053","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Neurosci."],"abstract":"<jats:p>Mouse models of inherited retinal degenerative diseases such as retinitis pigmentosa are characterized by degeneration of photoreceptors, which hinders the generation of signal to be transmitted to the visual cortex. By monitoring Ca<jats:sup>2+<\/jats:sup>-bioluminescence neural activity, we quantified changes in visual cortical activities in response to visual stimuli in RD10 mice during progression of retinal degeneration, which correlated with progressive deteriorations of electro-retinography signal from the eyes. The number of active neurons in the visual cortex, the intensity of Ca<jats:sup>2+<\/jats:sup>-bioluminescence response, and neural activation parameter showed progressive deterioration during aging. Further, we correlated the thinning of retina as measured by Optical Coherence Tomography with the decrease in visual cortical activities as retinal degeneration progressed. The present study establishes Ca<jats:sup>2+<\/jats:sup>-bioluminescence monitoring as a longitudinal imaging modality to characterize activities in visual cortex of retinal degenerative disease models and therapeutic interventions.<\/jats:p>","DOI":"10.3389\/fnins.2021.750684","type":"journal-article","created":{"date-parts":[[2021,10,4]],"date-time":"2021-10-04T06:55:50Z","timestamp":1633330550000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":2,"title":["Monitoring Visual Cortical Activities During Progressive Retinal Degeneration Using Functional Bioluminescence Imaging"],"prefix":"10.3389","volume":"15","author":[{"given":"Darryl","family":"Narcisse","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sourajit M.","family":"Mustafi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael","family":"Carlson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sanghoon","family":"Kim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Subrata","family":"Batabyal","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Weldon","family":"Wright","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Samarendra K.","family":"Mohanty","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2021,10,4]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1101\/509323","article-title":"Bioluminescent Multi-Characteristic Opsin for monitoring visual cortical activity upon optical stimulation.","author":"Batabyal","year":"2018","journal-title":"bioRxiv"},{"key":"B2","doi-asserted-by":"publisher","first-page":"e2218","DOI":"10.21769\/BioProtoc.2218","article-title":"Assessment of murine retinal function by electroretinography.","volume":"7","author":"Benchorin","year":"2017","journal-title":"Bio Protoc."},{"key":"B3","doi-asserted-by":"publisher","first-page":"4005","DOI":"10.1523\/JNEUROSCI.23-10-04005.2003","article-title":"Visual cortex activity in early and late blind people.","volume":"23","author":"Burton","year":"2003","journal-title":"J. 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