{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T16:08:13Z","timestamp":1753891693248,"version":"3.41.2"},"reference-count":37,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2024,2,21]],"date-time":"2024-02-21T00:00:00Z","timestamp":1708473600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Neurosci."],"abstract":"<jats:p>The Wnt pathway plays critical roles in neurogenesis. The expression of <jats:italic>Axin2<\/jats:italic> is induced by Wnt\/\u03b2-catenin signaling, making this gene a reliable indicator of canonical Wnt activity. We employed pulse-chase genetic lineage tracing with the <jats:italic>Axin2-CreERT2<\/jats:italic> allele to follow the fate of <jats:italic>Axin2<\/jats:italic>+ lineage in the adult hippocampal formation. We found <jats:italic>Axin2<\/jats:italic> expressed in astrocytes, neurons and endothelial cells, as well as in the choroid plexus epithelia. Simultaneously with the induction of <jats:italic>Axin2<\/jats:italic> fate mapping by tamoxifen, we marked the dividing cells with 5-ethynyl-2\u2032-deoxyuridine (EdU). Tamoxifen induction led to a significant increase in labeled dentate gyrus granule cells three months later. However, none of these neurons showed any EdU signal. Conversely, six months after the pulse-chase labeling with tamoxifen\/EdU, we identified granule neurons that were positive for both EdU and tdTomato lineage tracer in each animal. Our data indicates that <jats:italic>Axin2<\/jats:italic> is expressed at multiple stages of adult granule neuron differentiation. Furthermore, these findings suggest that the integration process of adult-born neurons from specific cell lineages may require more time than previously thought.<\/jats:p>","DOI":"10.3389\/fnins.2024.1353142","type":"journal-article","created":{"date-parts":[[2024,2,21]],"date-time":"2024-02-21T05:50:12Z","timestamp":1708494612000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Exploring the dynamics of adult Axin2 cell lineage integration into dentate gyrus granule neurons"],"prefix":"10.3389","volume":"18","author":[{"given":"Khadijeh A.","family":"Sharifi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Faraz","family":"Farzad","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sauson","family":"Soldozy","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matthew R.","family":"DeWitt","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Richard J.","family":"Price","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jason","family":"Sheehan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. Yashar S.","family":"Kalani","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Petr","family":"Tvrdik","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2024,2,21]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"523","DOI":"10.1152\/physrev.00055.2003","article-title":"Adult neurogenesis: from precursors to network and physiology","volume":"85","author":"Abrous","year":"2005","journal-title":"Physiol. 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