{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T16:11:13Z","timestamp":1753891873693,"version":"3.41.2"},"reference-count":88,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2025,5,27]],"date-time":"2025-05-27T00:00:00Z","timestamp":1748304000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Neurosci."],"abstract":"<jats:sec><jats:title>Introduction<\/jats:title><jats:p>Stress is a risk factor for ethanol use disorders, which has been modeled by chronic intermittent ethanol (CIE) vapor exposure. Repeated stress alters CB<jats:sub>1<\/jats:sub> receptor signaling, which could influence ethanol-related behaviors. Striatal CB<jats:sub>1<\/jats:sub> receptors regulate D<jats:sub>1<\/jats:sub>-medium spiny neurons (D<jats:sub>1<\/jats:sub>-MSNs), involved in goal-directed behaviors and stress responses. This study tested the hypothesis that predator odor stress interacts with CIE exposure to: (1) increase or accelerate CIE-induced escalation in ethanol intake, (2) increase plasma corticosterone levels, and (3) increase the expression or co-localization of CB<jats:sub>1<\/jats:sub> receptors, D<jats:sub>1<\/jats:sub>-MSNs, and Fos neuronal activation marker in the nucleus accumbens (NAc), dorsomedial (DMS), and dorsolateral (DLS) striatum.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Male C57BL\/6J mice underwent three cycles of 4 days CIE or air exposure, alternated with 5 days ethanol access. During the last two cycles, mice were exposed to predator odor or control bedding before each drinking session. Following the last stressor, brains were processed for RNAscope to label <jats:italic>Cnr1<\/jats:italic> (encodes CB<jats:sub>1<\/jats:sub>), <jats:italic>Drd1<\/jats:italic> (D<jats:sub>1<\/jats:sub>), and <jats:italic>Fos<\/jats:italic> (Fos).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>As hypothesized, predator odor accelerated the CIE-induced increase in ethanol intake. Contrary to our expectations, CIE did not alter corticosterone levels after the final stressor. Compared to control bedding, predator odor reduced the percentage of <jats:italic>Fos<\/jats:italic>+ and triple-labeled <jats:italic>Cnr1\/Drd1\/Fos<\/jats:italic>+ cells in NAc, but not dorsal striatum. In addition, CIE vs. Air exposure, increased percentages of <jats:italic>Fos<\/jats:italic>+, double-labeled <jats:italic>Cnr1\/Fos<\/jats:italic>+, <jats:italic>Drd1\/Fos<\/jats:italic>+, and triple <jats:italic>Cnr1\/Drd1\/Fos<\/jats:italic>+ cells in the NAc, but not DMS or DLS.<\/jats:p><\/jats:sec><jats:sec><jats:title>Discussion<\/jats:title><jats:p>Thus, CIE and stress elicited opposite neuroactivational effects on CB<jats:sub>1<\/jats:sub>-regulated D<jats:sub>1<\/jats:sub>-MSNs of the NAc. The role of these changes in stress- and CIE-augmented drinking warrants further investigation.<\/jats:p><\/jats:sec>","DOI":"10.3389\/fnins.2025.1568952","type":"journal-article","created":{"date-parts":[[2025,5,27]],"date-time":"2025-05-27T05:24:05Z","timestamp":1748323445000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Impacts of chronic intermittent ethanol vapor and predator odor on ethanol intake and striatal D1 and CB1 cannabinoid receptor-expressing medium spiny neurons"],"prefix":"10.3389","volume":"19","author":[{"given":"Cristiane Aparecida","family":"Favoretto","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Allyson","family":"Nguyen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gabriela R.","family":"Chacon","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amanda J.","family":"Roberts","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tali","family":"Nadav","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Saumya","family":"Ranjan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luisa Becker","family":"Bertotto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F\u00e1bio Cardoso","family":"Cruz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eric P.","family":"Zorrilla","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2025,5,27]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"146485","DOI":"10.1016\/j.brainres.2019.146485","article-title":"Opposed cannabinoid 1 receptor (CB1R) expression in the prefrontal cortex vs. nucleus accumbens is associated with alcohol consumption in male rats.","volume":"1725","author":"Amancio-Belmont","year":"2019","journal-title":"Brain Res."},{"key":"B2","doi-asserted-by":"publisher","first-page":"16878","DOI":"10.1038\/s41598-017-17204-5","article-title":"QuPath: Open source software for digital pathology image analysis.","volume":"7","author":"Bankhead","year":"2017","journal-title":"Sci. Rep."},{"key":"B3","doi-asserted-by":"publisher","first-page":"1829","DOI":"10.1097\/01.alc.0000149977.95306.3a","article-title":"Increased ethanol drinking after repeated chronic ethanol exposure and withdrawal experience in C57BL\/6 mice.","volume":"28","author":"Becker","year":"2004","journal-title":"Alcohol Clin. Exp. Res."},{"key":"B4","doi-asserted-by":"publisher","first-page":"2498","DOI":"10.1038\/npp.2013.153","article-title":"Rimonabant precipitates anxiety in rats withdrawn from palatable food: Role of the central amygdala.","volume":"38","author":"Blasio","year":"2013","journal-title":"Neuropsychopharmacology"},{"key":"B5","doi-asserted-by":"publisher","first-page":"353","DOI":"10.1038\/s41386-023-01672-7","article-title":"Increasing ecological validity in neuropsychopharmacology research on the relationship between alcohol use and uncertain stressors.","volume":"49","author":"Bradford","year":"2024","journal-title":"Neuropsychopharmacology"},{"key":"B6","doi-asserted-by":"publisher","first-page":"918","DOI":"10.1016\/j.biopsych.2016.05.016","article-title":"Distinct synaptic strengthening of the striatal direct and indirect pathways drives alcohol consumption.","volume":"81","author":"Cheng","year":"2017","journal-title":"Biol. Psychiatry"},{"key":"B7","doi-asserted-by":"publisher","first-page":"813","DOI":"10.1016\/j.pbb.2007.03.009","article-title":"Dependence-induced increases in ethanol self-administration in mice are blocked by the CRF1 receptor antagonist antalarmin and by CRF1 receptor knockout.","volume":"86","author":"Chu","year":"2007","journal-title":"Pharmacol. Biochem. Behav."},{"key":"B8","doi-asserted-by":"publisher","first-page":"741","DOI":"10.1016\/j.alcohol.2014.07.015","article-title":"Environmental stressors influence limited-access ethanol consumption by C57BL\/6J mice in a sex-dependent manner.","volume":"48","author":"Cozzoli","year":"2014","journal-title":"Alcohol"},{"key":"B9","doi-asserted-by":"publisher","first-page":"14783","DOI":"10.1073\/pnas.1308198110","article-title":"Chronic alcohol produces neuroadaptations to prime dorsal striatal learning.","volume":"110","author":"DePoy","year":"2013","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"B10","doi-asserted-by":"publisher","first-page":"124","DOI":"10.1016\/j.neuroscience.2024.11.037","article-title":"Impacts of maternal separation stress on ethanol intake and endocannabinoid system in adolescent mice.","volume":"565","author":"Favoretto","year":"2025","journal-title":"Neuroscience"},{"key":"B11","doi-asserted-by":"publisher","first-page":"1175514","DOI":"10.3389\/fnins.2023.1175514","article-title":"Involvement of brain cell phenotypes in stress-vulnerability and resilience.","volume":"17","author":"Favoretto","year":"2023","journal-title":"Front. Neurosci."},{"key":"B12","doi-asserted-by":"publisher","first-page":"355","DOI":"10.1016\/bs.irn.2024.08.004","article-title":"Animal models for studying therapeutic targets and treatments for alcohol use disorder.","volume":"178","author":"Favoretto","year":"2024","journal-title":"Int. Rev. Neurobiol."},{"key":"B13","doi-asserted-by":"publisher","first-page":"509","DOI":"10.1038\/s41386-020-00904-4","article-title":"Pituitary adenylate cyclase-activating polypeptide (PACAP) modulates dependence-induced alcohol drinking and anxiety-like behavior in male rats.","volume":"46","author":"Ferragud","year":"2021","journal-title":"Neuropsychopharmacology"},{"key":"B14","doi-asserted-by":"publisher","first-page":"327","DOI":"10.1007\/s40429-023-00540-0","article-title":"Traumatic stress-enhanced alcohol drinking: Sex differences and animal model perspectives.","volume":"11","author":"Finn","year":"2024","journal-title":"Curr. Addict. Rep."},{"key":"B15","doi-asserted-by":"publisher","first-page":"33","DOI":"10.1016\/j.alcohol.2018.02.004","article-title":"Sex differences in the synergistic effect of prior binge drinking and traumatic stress on subsequent ethanol intake and neurochemical responses in adult C57BL\/6J mice.","volume":"71","author":"Finn","year":"2018","journal-title":"Alcohol"},{"key":"B16","doi-asserted-by":"publisher","first-page":"21","DOI":"10.1016\/j.pnpbp.2011.12.004","article-title":"Cannabinoid modulation of the dopaminergic circuitry: Implications for limbic and striatal output.","volume":"38","author":"Fitzgerald","year":"2012","journal-title":"Prog. Neuropsychopharmacol. Biol. Psychiatry"},{"key":"B17","doi-asserted-by":"publisher","first-page":"256","DOI":"10.3389\/fnins.2013.00256","article-title":"Cannabinoid-hypocretin cross-talk in the central nervous system: What we know so far.","volume":"7","author":"Flores","year":"2013","journal-title":"Front. Neurosci."},{"key":"B18","doi-asserted-by":"publisher","first-page":"645","DOI":"10.1016\/j.biopsych.2016.09.007","article-title":"Emerging role for nucleus accumbens medium spiny neuron subtypes in depression.","volume":"81","author":"Francis","year":"2017","journal-title":"Biol. Psychiatry"},{"key":"B19","doi-asserted-by":"publisher","first-page":"509","DOI":"10.1017\/dmp.2015.67","article-title":"Distress of routine activities and perceived safety associated with post-traumatic stress, depression, and alcohol use: 2002 Washington, DC, sniper attacks.","volume":"9","author":"Fullerton","year":"2015","journal-title":"Disaster Med. Public Health Prep."},{"key":"B20","doi-asserted-by":"publisher","first-page":"109549","DOI":"10.1016\/j.neuropharm.2023.109549","article-title":"Cannabidiol regulates behavioral and brain alterations induced by spontaneous alcohol withdrawal.","volume":"233","author":"Gasparyan","year":"2023","journal-title":"Neuropharmacology"},{"key":"B21","doi-asserted-by":"publisher","first-page":"299","DOI":"10.1016\/s0306-4522(96)00428-9","article-title":"Cannabinoid receptors in the human brain: A detailed anatomical and quantitative autoradiographic study in the fetal, neonatal and adult human brain.","volume":"77","author":"Glass","year":"1997","journal-title":"Neuroscience"},{"key":"B22","doi-asserted-by":"publisher","first-page":"339","DOI":"10.1016\/j.bbr.2014.05.040","article-title":"Subchronic and mild social defeat stress accelerates food intake and body weight gain with polydipsia-like features in mice.","volume":"270","author":"Goto","year":"2014","journal-title":"Behav. Brain Res."},{"key":"B23","doi-asserted-by":"publisher","first-page":"1380","DOI":"10.1176\/appi.ps.54.10.1380","article-title":"Posttraumatic stress disorder, alcohol use, and perceived safety after the terrorist attack on the pentagon.","volume":"54","author":"Grieger","year":"2003","journal-title":"Psychiatr. Serv."},{"key":"B24","doi-asserted-by":"publisher","first-page":"1893","DOI":"10.1111\/j.1530-0277.2009.01027.x","article-title":"Intensity and duration of chronic ethanol exposure is critical for subsequent escalation of voluntary ethanol drinking in mice.","volume":"33","author":"Griffin","year":"","journal-title":"Alcohol Clin. Exp. Res."},{"key":"B25","doi-asserted-by":"publisher","first-page":"569","DOI":"10.1007\/s00213-008-1324-3","article-title":"Repeated cycles of chronic intermittent ethanol exposure in mice increases voluntary ethanol drinking and ethanol concentrations in the nucleus accumbens.","volume":"201","author":"Griffin","year":"","journal-title":"Psychopharmacology (Berl)"},{"key":"B26","doi-asserted-by":"publisher","first-page":"44","DOI":"10.1016\/j.alcohol.2022.10.004","article-title":"Alcohol dependence and the ventral hippocampal influence on alcohol drinking in male mice.","volume":"106","author":"Griffin","year":"2023","journal-title":"Alcohol"},{"key":"B27","doi-asserted-by":"publisher","first-page":"25","DOI":"10.1016\/j.bbr.2015.10.049","article-title":"Chronic mild stress in submissive mice: Marked polydipsia and social avoidance without hedonic deficit in the sucrose preference test.","volume":"298","author":"Gross","year":"2016","journal-title":"Behav. Brain Res."},{"key":"B28","doi-asserted-by":"publisher","first-page":"169","DOI":"10.1111\/j.1369-1600.2009.00194.x","article-title":"Acute withdrawal, protracted abstinence and negative affect in alcoholism: Are they linked?","volume":"15","author":"Heilig","year":"2010","journal-title":"Addict. Biol."},{"key":"B29","doi-asserted-by":"publisher","first-page":"2322","DOI":"10.1111\/j.1471-4159.2008.05567.x","article-title":"Regional alterations in the endocannabinoid system in an animal model of depression: Effects of concurrent antidepressant treatment.","volume":"106","author":"Hill","year":"2008","journal-title":"J. Neurochem."},{"key":"B30","doi-asserted-by":"publisher","first-page":"380","DOI":"10.1016\/j.smim.2014.04.001","article-title":"Stress regulates endocannabinoid-CB1 receptor signaling.","volume":"26","author":"Hillard","year":"2014","journal-title":"Semin. Immunol."},{"key":"B31","doi-asserted-by":"publisher","first-page":"540","DOI":"10.1111\/acer.13584","article-title":"Effects of withdrawal from chronic intermittent ethanol exposure on sleep characteristics of female and male mice.","volume":"42","author":"Huitron-Resendiz","year":"2018","journal-title":"Alcohol Clin. Exp. Res."},{"key":"B32","doi-asserted-by":"publisher","first-page":"184","DOI":"10.1016\/j.neuroscience.2014.06.063","article-title":"Cell type-specific synaptic encoding of ethanol exposure in the nucleus accumbens shell.","volume":"277","author":"Jeanes","year":"2014","journal-title":"Neuroscience"},{"key":"B33","doi-asserted-by":"publisher","first-page":"788","DOI":"10.1038\/s41593-020-0660-4","article-title":"Using bayes factor hypothesis testing in neuroscience to establish evidence of absence.","volume":"23","author":"Keysers","year":"2020","journal-title":"Nat. Neurosci."},{"key":"B34","doi-asserted-by":"publisher","first-page":"1886","DOI":"10.1111\/acer.13484","article-title":"CRF1 receptor-dependent increases in irritability-like behavior during abstinence from chronic intermittent ethanol vapor exposure.","volume":"41","author":"Kimbrough","year":"2017","journal-title":"Alcohol Clin. Exp. Res."},{"key":"B35","doi-asserted-by":"publisher","first-page":"1939","DOI":"10.1097\/01.alc.0000187592.57853.12","article-title":"Acute alcohol withdrawal is associated with c-Fos expression in the basal ganglia and associated circuitry: C57bl\/6j and DBA\/2J inbred mouse strain analyses.","volume":"29","author":"Kozell","year":"2005","journal-title":"Alcohol Clin. Exp. Res."},{"key":"B36","doi-asserted-by":"publisher","first-page":"41","DOI":"10.3389\/fnana.2011.00041","article-title":"The striatal balancing act in drug addiction: Distinct roles of direct and indirect pathway medium spiny neurons.","volume":"5","author":"Lobo","year":"2011","journal-title":"Front. Neuroanat."},{"key":"B37","doi-asserted-by":"publisher","first-page":"18381","DOI":"10.1523\/JNEUROSCI.1875-13.2013","article-title":"\u0394FosB induction in striatal medium spiny neuron subtypes in response to chronic pharmacological, emotional, and optogenetic stimuli.","volume":"33","author":"Lobo","year":"2013","journal-title":"J. Neurosci."},{"key":"B38","doi-asserted-by":"publisher","first-page":"920","DOI":"10.1111\/j.1369-1600.2012.00460.x","article-title":"Stress history increases alcohol intake in relapse: Relation to phosphodiesterase 10A.","volume":"17","author":"Logrip","year":"2012","journal-title":"Addict. Biol."},{"key":"B39","doi-asserted-by":"publisher","first-page":"552","DOI":"10.1016\/j.neuropharm.2011.07.007","article-title":"Stress modulation of drug self-administration: Implications for addiction comorbidity with post-traumatic stress disorder.","volume":"62","author":"Logrip","year":"2012","journal-title":"Neuropharmacology"},{"key":"B40","doi-asserted-by":"publisher","first-page":"17","DOI":"10.1016\/j.alcohol.2015.11.010","article-title":"Effect of different stressors on voluntary ethanol intake in ethanol-dependent and nondependent C57BL\/6J mice.","volume":"51","author":"Lopez","year":"2016","journal-title":"Alcohol"},{"key":"B41","doi-asserted-by":"publisher","first-page":"688","DOI":"10.1007\/s00213-005-0026-3","article-title":"Effect of pattern and number of chronic ethanol exposures on subsequent voluntary ethanol intake in C57BL\/6J mice.","volume":"181","author":"Lopez","year":"2005","journal-title":"Psychopharmacology (Berl)"},{"key":"B42","doi-asserted-by":"publisher","first-page":"821","DOI":"10.1038\/s41386-023-01529-z","article-title":"Chronic MAP4343 reverses escalated alcohol drinking in a mouse model of alcohol use disorder.","volume":"48","author":"Macedo","year":"2023","journal-title":"Neuropsychopharmacology"},{"key":"B43","doi-asserted-by":"publisher","first-page":"40","DOI":"10.3389\/fncel.2016.00040","article-title":"Chronic intermittent ethanol exposure alters stress effects on (3\u03b1,5\u03b1)-3-hydroxy-pregnan-20-one (3\u03b1,5\u03b1-THP) immunolabeling of amygdala neurons in C57BL\/6J mice.","volume":"10","author":"Maldonado-Devincci","year":"2016","journal-title":"Front. Cell Neurosci."},{"key":"B44","doi-asserted-by":"publisher","first-page":"547","DOI":"10.1097\/FBP.0000000000000068","article-title":"Consequences of early life stress on the expression of endocannabinoid-related genes in the rat brain.","volume":"25","author":"Marco","year":"2014","journal-title":"Behav. Pharmacol."},{"key":"B45","doi-asserted-by":"publisher","first-page":"7940","DOI":"10.3390\/ijms22157940","article-title":"Memantine prevents the win 55,212-2 evoked cross-priming of ethanol-induced Conditioned Place Preference (CPP).","volume":"22","author":"Marszalek-Grabska","year":"2021","journal-title":"Int. J. Mol. Sci."},{"key":"B46","doi-asserted-by":"publisher","first-page":"17316","DOI":"10.3390\/ijms242417316","article-title":"Omega-3 recovers cannabinoid 1 receptor expression in the adult mouse brain after adolescent binge drinking.","volume":"24","author":"Mart\u00edn-Llorente","year":"2023","journal-title":"Int. J. Mol. Sci."},{"key":"B47","doi-asserted-by":"publisher","first-page":"370","DOI":"10.1037\/h0054346","article-title":"A theory of human motivation.","volume":"50","author":"Maslow","year":"1943","journal-title":"Psychol. Rev."},{"key":"B48","doi-asserted-by":"publisher","first-page":"315","DOI":"10.3389\/fpsyt.2020.00315","article-title":"Endocannabinoid system components as potential biomarkers in psychiatry.","volume":"11","author":"Navarrete","year":"2020","journal-title":"Front. Psychiatry"},{"key":"B49","doi-asserted-by":"publisher","first-page":"1676","DOI":"10.1097\/01.alc.0000145781.11923.4e","article-title":"Enhanced alcohol self-administration after intermittent versus continuous alcohol vapor exposure.","volume":"28","author":"O\u2019Dell","year":"2004","journal-title":"Alcohol Clin. Exp. Res."},{"key":"B50","doi-asserted-by":"publisher","first-page":"100269","DOI":"10.1016\/j.ynstr.2020.100269","article-title":"novel mouse model for vulnerability to alcohol dependence induced by early-life adversity.","volume":"13","author":"Okhuarobo","year":"2020","journal-title":"Neurobiol. Stress"},{"key":"B51","doi-asserted-by":"publisher","first-page":"1358","DOI":"10.1038\/sj.npp.1301237","article-title":"Gene transcription alterations associated with decrease of ethanol intake induced by naltrexone in the brain of Wistar rats.","volume":"32","author":"Oliva","year":"2007","journal-title":"Neuropsychopharmacology"},{"key":"B52","doi-asserted-by":"publisher","first-page":"100634","DOI":"10.1016\/j.ynstr.2024.100634","article-title":"Predator odor stress reactivity, alcohol drinking and the endocannabinoid system.","volume":"30","author":"Ornelas","year":"2024","journal-title":"Neurobiol. Stress"},{"key":"B53","doi-asserted-by":"publisher","first-page":"88","DOI":"10.1093\/alcalc\/agh036","article-title":"Chronic ethanol consumption regulates cannabinoid CB1 receptor gene expression in selected regions of rat brain.","volume":"39","author":"Ortiz","year":"2004","journal-title":"Alcohol. Alcohol."},{"key":"B54","doi-asserted-by":"publisher","first-page":"156","DOI":"10.1038\/s41398-018-0215-6","article-title":"Peripubertal stress increases play fighting at adolescence and modulates nucleus accumbens CB1 receptor expression and mitochondrial function in the amygdala.","volume":"8","author":"Papilloud","year":"2018","journal-title":"Transl. Psychiatry"},{"key":"B55","doi-asserted-by":"publisher","first-page":"231","DOI":"10.1016\/j.physbeh.2012.06.017","article-title":"Effects of CB1 and CRF1 receptor antagonists on binge-like eating in rats with limited access to a sweet fat diet: Lack of withdrawal-like responses.","volume":"107","author":"Parylak","year":"2012","journal-title":"Physiol. Behav."},{"key":"B56","doi-asserted-by":"publisher","first-page":"114","DOI":"10.3389\/fnbeh.2020.00114","article-title":"Chronic intermittent ethanol exposure increases ethanol consumption following traumatic stress exposure in mice.","volume":"14","author":"Piggott","year":"2020","journal-title":"Front. Behav. Neurosci."},{"key":"B57","doi-asserted-by":"publisher","first-page":"271","DOI":"10.1002\/cne.10868","article-title":"The associative striatum: Organization of cortical projections to the dorsocentral striatum in rats.","volume":"467","author":"Reep","year":"2003","journal-title":"J. Comp. Neurol."},{"key":"B58","doi-asserted-by":"publisher","first-page":"211","DOI":"10.1038\/s41467-017-02615-9","article-title":"Chronic alcohol exposure disrupts top-down control over basal ganglia action selection to produce habits.","volume":"9","author":"Renteria","year":"2018","journal-title":"Nat. Commun."},{"key":"B59","doi-asserted-by":"publisher","first-page":"164","DOI":"10.1016\/j.neuropharm.2016.03.004","article-title":"Selective alterations of NMDAR function and plasticity in D1 and D2 medium spiny neurons in the nucleus accumbens shell following chronic intermittent ethanol exposure.","volume":"112","author":"Renteria","year":"2017","journal-title":"Neuropharmacology"},{"key":"B60","doi-asserted-by":"publisher","first-page":"1574","DOI":"10.1111\/acer.13444","article-title":"Stress facilitates the development of cognitive dysfunction after chronic ethanol exposure.","volume":"41","author":"Rodberg","year":"2017","journal-title":"Alcohol Clin. Exp. Res."},{"key":"B61","doi-asserted-by":"publisher","first-page":"296","DOI":"10.1016\/j.neuroscience.2012.07.071","article-title":"Maternal separation and proclivity for ethanol intake: A potential role of the endocannabinoid system in rats.","volume":"223","author":"Romano-L\u00f3pez","year":"2012","journal-title":"Neuroscience"},{"key":"B62","doi-asserted-by":"publisher","first-page":"354","DOI":"10.1016\/j.drugalcdep.2008.08.004","article-title":"Effects of a short-term exposure to alcohol in rats on FAAH enzyme and CB1 receptor in different brain areas.","volume":"99","author":"Rubio","year":"2009","journal-title":"Drug Alcohol Depend."},{"key":"B63","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-030-46380-9_7","article-title":"Generalized linear models","author":"Schmettow","year":"2021","journal-title":"New statistics for design researchers. Human-computer interaction series"},{"key":"B64","doi-asserted-by":"publisher","first-page":"1840","DOI":"10.1038\/s41386-018-0055-3","article-title":"Deficient endocannabinoid signaling in the central amygdala contributes to alcohol dependence-related anxiety-like behavior and excessive alcohol intake.","volume":"43","author":"Serrano","year":"2018","journal-title":"Neuropsychopharmacology"},{"key":"B65","doi-asserted-by":"publisher","first-page":"343","DOI":"10.1007\/s002130100917","article-title":"How does stress increase risk of drug abuse and relapse?","volume":"158","author":"Sinha","year":"2001","journal-title":"Psychopharmacology (Berl)"},{"key":"B66","doi-asserted-by":"publisher","first-page":"105","DOI":"10.1196\/annals.1441.030","article-title":"Chronic stress, drug use, and vulnerability to addiction.","volume":"1141","author":"Sinha","year":"2008","journal-title":"Ann. N. Y. Acad. Sci."},{"key":"B67","doi-asserted-by":"publisher","first-page":"e12804","DOI":"10.1111\/adb.12804","article-title":"Dynamic c-Fos changes in mouse brain during acute and protracted withdrawal from chronic intermittent ethanol exposure and relapse drinking.","volume":"25","author":"Smith","year":"2020","journal-title":"Addict. Biol."},{"key":"B68","doi-asserted-by":"publisher","first-page":"17","DOI":"10.1016\/j.psyneuen.2017.06.006","article-title":"Abstinence from prolonged ethanol exposure affects plasma corticosterone, glucocorticoid receptor signaling and stress-related behaviors.","volume":"84","author":"Somkuwar","year":"2017","journal-title":"Psychoneuroendocrinology"},{"key":"B69","doi-asserted-by":"publisher","first-page":"937","DOI":"10.1016\/j.neuroscience.2007.11.039","article-title":"Cat odor, but not trimethylthiazoline (fox odor), activates accessory olfactory and defense-related brain regions in rats.","volume":"151","author":"Staples","year":"2008","journal-title":"Neuroscience"},{"key":"B70","doi-asserted-by":"publisher","first-page":"3093","DOI":"10.1038\/s41380-020-00920-2","article-title":"Importance of sex and trauma context on circulating cytokines and amygdalar GABAergic signaling in a comorbid model of posttraumatic stress and alcohol use disorders.","volume":"26","author":"Steinman","year":"2021","journal-title":"Mol. Psychiatry"},{"key":"B71","doi-asserted-by":"publisher","first-page":"17","DOI":"10.3389\/fnsys.2016.00017","article-title":"The striatum and subthalamic nucleus as independent and collaborative structures in motor control.","volume":"10","author":"Tewari","year":"2016","journal-title":"Front. Syst. Neurosci."},{"key":"B72","doi-asserted-by":"publisher","first-page":"19","DOI":"10.1111\/j.1530-0277.2010.01318.x","article-title":"Upregulation of cannabinoid type 1 receptors in dopamine D2 receptor knockout mice is reversed by chronic forced ethanol consumption.","volume":"35","author":"Thanos","year":"2011","journal-title":"Alcohol Clin. Exp. Res."},{"key":"B73","doi-asserted-by":"publisher","first-page":"e12684","DOI":"10.1111\/gbb.12684","article-title":"Exposure to the predator odor TMT induces early and late differential gene expression related to stress and excitatory synaptic function throughout the brain in male rats.","volume":"19","author":"Tyler","year":"2020","journal-title":"Genes Brain Behav."},{"key":"B74","doi-asserted-by":"publisher","first-page":"1494","DOI":"10.1097\/01.ALC.0000033120.51856.F0","article-title":"Increased ethanol self-administration and anxiety-like behavior during acute ethanol withdrawal and protracted abstinence: Regulation by corticotropin-releasing factor.","volume":"26","author":"Valdez","year":"2002","journal-title":"Alcohol Clin. Exp. Res."},{"key":"B75","doi-asserted-by":"publisher","first-page":"301","DOI":"10.1016\/s0031-9384(01)00458-9","article-title":"Persistent suppression of ethanol self-administration by brief social stress in rats and increased startle response as index of withdrawal.","volume":"73","author":"van Erp","year":"2001","journal-title":"Physiol. Behav."},{"key":"B76","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.ibror.2018.05.002","article-title":"Effects of an early life experience on rat brain cannabinoid receptors in adolescence and adulthood.","volume":"5","author":"Vangopoulou","year":"2018","journal-title":"IBRO Rep."},{"key":"B77","doi-asserted-by":"publisher","DOI":"10.1007\/3-540-26573-2_12","article-title":"Retrograde signalling by endocannabinoids","volume":"168","author":"Vaughan","year":"2005","journal-title":"Cannabinoids. Handbook of experimental pharmacology"},{"key":"B78","doi-asserted-by":"publisher","first-page":"7563","DOI":"10.1523\/JNEUROSCI.0069-12.2012","article-title":"Corticosteroid-dependent plasticity mediates compulsive alcohol drinking in rats.","volume":"32","author":"Vendruscolo","year":"2012","journal-title":"J. Neurosci."},{"key":"B79","doi-asserted-by":"publisher","first-page":"277","DOI":"10.1016\/j.alcohol.2013.08.006","article-title":"Operant alcohol self-administration in dependent rats: Focus on the vapor model.","volume":"48","author":"Vendruscolo","year":"2014","journal-title":"Alcohol"},{"key":"B80","doi-asserted-by":"publisher","first-page":"114","DOI":"10.3758\/BF03327014","article-title":"The relationship between tension reduction and ethanol consumption in rats.","volume":"10","author":"Volpicelli","year":"1982","journal-title":"Physiol. Psychol."},{"key":"B81","doi-asserted-by":"publisher","first-page":"3130","DOI":"10.1111\/bph.16197","article-title":"Sexually dimorphic effects of monoacylglycerol lipase inhibitor MJN110 on stress-related behaviour and drinking in marchigian sardinian alcohol-preferring rats.","volume":"180","author":"Vozella","year":"2023","journal-title":"Br. J. Pharmacol."},{"key":"B82","doi-asserted-by":"publisher","first-page":"11634","DOI":"10.1523\/JNEUROSCI.0003-15.2015","article-title":"Alcohol elicits functional and structural plasticity selectively in dopamine d1 receptor-expressing neurons of the dorsomedial striatum.","volume":"35","author":"Wang","year":"2015","journal-title":"J. Neurosci."},{"key":"B83","doi-asserted-by":"publisher","first-page":"1393","DOI":"10.1073\/pnas.0336351100","article-title":"Endocannabinoid signaling via cannabinoid receptor 1 is involved in ethanol preference and its age-dependent decline in mice.","volume":"100","author":"Wang","year":"2003","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"journal-title":"World health statistics 2018: Monitoring health for the SDG\u2019s (sustainable development goals). [s.l.: s.n.].","year":"2018","key":"B84"},{"key":"B85","doi-asserted-by":"publisher","DOI":"10.3791\/65320","article-title":"Chronic intermittent ethanol vapor exposure paired with two-bottle choice to model alcohol use disorder.","author":"Xiao","year":"2023","journal-title":"J. Vis. Exp."},{"key":"B86","doi-asserted-by":"publisher","first-page":"13","DOI":"10.3389\/fncir.2016.00013","article-title":"Thirst is associated with suppression of habenula output and active stress coping: Is there a role for a non-canonical vasopressin-glutamate pathway?","volume":"10","author":"Zhang","year":"2016","journal-title":"Front. Neural Circuits"},{"key":"B87","doi-asserted-by":"publisher","first-page":"1505","DOI":"10.1111\/j.1530-0277.2007.00456.x","article-title":"Remission and resurgence of anxiety-like behavior across protracted withdrawal stages in ethanol-dependent rats.","volume":"31","author":"Zhao","year":"2007","journal-title":"Alcohol Clin. Exp. Res."},{"key":"B88","doi-asserted-by":"publisher","first-page":"8058093","DOI":"10.1155\/2016\/8058093","article-title":"Safety needs mediate stressful events induced mental disorders.","volume":"2016","author":"Zheng","year":"2016","journal-title":"Neural Plast"}],"container-title":["Frontiers in Neuroscience"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fnins.2025.1568952\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,5,27]],"date-time":"2025-05-27T05:24:17Z","timestamp":1748323457000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fnins.2025.1568952\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,5,27]]},"references-count":88,"alternative-id":["10.3389\/fnins.2025.1568952"],"URL":"https:\/\/doi.org\/10.3389\/fnins.2025.1568952","relation":{},"ISSN":["1662-453X"],"issn-type":[{"type":"electronic","value":"1662-453X"}],"subject":[],"published":{"date-parts":[[2025,5,27]]},"article-number":"1568952"}}