{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T12:22:37Z","timestamp":1782217357868,"version":"3.54.5"},"reference-count":51,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,1,17]],"date-time":"2022-01-17T00:00:00Z","timestamp":1642377600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Mol. Neurosci."],"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>DNA methylation at CpG sites is a vital epigenetic modification of the human genome affecting gene expression, and potentially, health outcomes. However, evidence is just budding on the effects of aerobic exercise-induced adaptation on DNA methylation in older mild cognitively impaired (MCI) elderly African American (AAs). Therefore, we examined the effects of a 6-month aerobic exercise-intervention on genome-wide DNA methylation in elderly AA MCI volunteers.<\/jats:p><\/jats:sec><jats:sec><jats:title>Design<\/jats:title><jats:p>Elderly AA volunteers confirmed MCI assigned into a 6-month program of aerobic exercise (eleven participants) underwent a 40-min supervised-training 3-times\/week and controls (eight participants) performed stretch training. Participants had maximal oxygen consumption (VO<jats:sub>2<\/jats:sub>max) test and Genome-wide methylation levels at CpG sites using the Infinium HumanMethylation450 BeadChip assay at baseline and after a 6-month exercise program. We computed false discovery rates (FDR) using Sidak to account for multiplicity of tests and performed quantitative real-time polymerase chain-reaction (qRT-PCR) to confirm the effects of DNA methylations on expression levels of the top 5 genes among the aerobic participants. CpG sites identified from aerobic-exercise participants were similarly analyzed by the stretch group to quantify the effects of exercise-induced methylation changes among the group of stretch participants.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Eleven MCI participants (aerobic: 73% females; mean age 72.3 \u00b1 6.6 years) and eight MCI participants (stretch: 75% female; mean age 70.6 \u00b1 6.7 years) completed the training. Aerobic exercise-training was associated with increases in VO<jats:sub>2<\/jats:sub>max and with global hypo- and hypermethylation changes. The most notable finding was CpG hypomethylation within the body of the <jats:italic>VPS52<\/jats:italic> gene (<jats:italic>P<\/jats:italic> = 5.4 \u00d7 10<jats:sup>\u201326<\/jats:sup>), a Golgi-associated protein, involved in intracellular protein trafficking including amyloid precursor protein. qRT-PCR confirmed a nearly twofold increased expression of <jats:italic>VPS52<\/jats:italic>. Other top findings with FDR <jats:italic>q<\/jats:italic>-value &amp;lt; 10<jats:sup>\u20135<\/jats:sup>, include hypomethylations of <jats:italic>SCARB1<\/jats:italic> (8.8 \u00d7 10<jats:sup>\u201325<\/jats:sup>), <jats:italic>ARTN<\/jats:italic> (6.1 \u00d7 10<jats:sup>\u201325<\/jats:sup>), <jats:italic>NR1H2<\/jats:italic> (2.1 \u00d7 10<jats:sup>\u201318<\/jats:sup>) and <jats:italic>PPP2R5D<\/jats:italic> (9.8 \u00d7 10<jats:sup>\u201318<\/jats:sup>).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>We conclude that genome-wide DNA methylation patterns is associated with exercise training-induced methylation changes. Identification of methylation changes around genes previously shown to interact with amyloid biology, intracellular protein trafficking, and lipoprotein regulations provide further support to the likely protective effect of exercise in MCI. Future studies in larger samples are needed to confirm our findings.<\/jats:p><\/jats:sec>","DOI":"10.3389\/fnmol.2021.752403","type":"journal-article","created":{"date-parts":[[2022,1,17]],"date-time":"2022-01-17T14:10:24Z","timestamp":1642428624000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":15,"title":["Aerobic Exercise Training-Induced Changes on DNA Methylation in Mild Cognitively Impaired Elderly African Americans: Gene, Exercise, and Memory Study - GEMS-I"],"prefix":"10.3389","volume":"14","author":[{"given":"Julius S.","family":"Ngwa","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Evaristus","family":"Nwulia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oyonumo","family":"Ntekim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fikru B.","family":"Bedada","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bernard","family":"Kwabi-Addo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sheeba","family":"Nadarajah","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven","family":"Johnson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William M.","family":"Southerland","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John","family":"Kwagyan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas O.","family":"Obisesan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2022,1,17]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1186\/s40880-016-0082-6","article-title":"Why some tumours trigger neovascularisation and others don\u2019t: the story thus far.","volume":"35","author":"Adighibe","year":"2016","journal-title":"Chin. 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