{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T15:04:55Z","timestamp":1780412695725,"version":"3.54.1"},"reference-count":86,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2023,5,17]],"date-time":"2023-05-17T00:00:00Z","timestamp":1684281600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Mol. Neurosci."],"abstract":"<jats:p><jats:italic>Octopus vulgaris<\/jats:italic> is a cephalopod mollusk and an active marine predator that has been at the center of a number of studies focused on the understanding of neural and biological plasticity. Studies on the machinery involved in e.g., learning and memory, regeneration, and neuromodulation are required to shed light on the conserved and\/or unique mechanisms that these animals have evolved. Analysis of gene expression is one of the most essential means to expand our understanding of biological machinery, and the selection of an appropriate set of reference genes is the prerequisite for the quantitative real-time polymerase chain reaction (qRT-PCR). Here we selected 77 candidate reference genes (RGs) from a pool of stable and relatively high-expressed transcripts identified from the full-length transcriptome of <jats:italic>O. vulgaris<\/jats:italic>, and we evaluated their expression stabilities in different tissues through <jats:italic>geNorm<\/jats:italic>, <jats:italic>NormFinder<\/jats:italic>, <jats:italic>Bestkeeper<\/jats:italic>, Delta-CT method, and <jats:italic>RefFinder<\/jats:italic>. Although various algorithms provided different assemblages of the most stable reference genes for the different kinds of tissues tested here, a comprehensive ranking revealed RGs specific to the nervous system (<jats:italic>Ov-RNF7<\/jats:italic> and <jats:italic>Ov-RIOK2<\/jats:italic>) and <jats:italic>Ov-EIF2A<\/jats:italic> and <jats:italic>Ov-CUL1<\/jats:italic> across all considered tissues. Furthermore, we validated RGs by assessing the expression profiles of nine target genes (<jats:italic>Ov-Naa15<\/jats:italic>, <jats:italic>Ov-Ltv1<\/jats:italic>, <jats:italic>Ov-CG9286<\/jats:italic>, <jats:italic>Ov-EIF3M<\/jats:italic>, <jats:italic>Ov-NOB1<\/jats:italic>, <jats:italic>Ov-CSDE1<\/jats:italic>, <jats:italic>Ov-Abi2<\/jats:italic>, <jats:italic>Ov-Homer2<\/jats:italic>, and <jats:italic>Ov-Snx20<\/jats:italic>) in different areas of the octopus nervous system (gastric ganglion, as control). Our study allowed us to identify the most extensive set of stable reference genes currently available for the nervous system and appendages of adult <jats:italic>O. vulgaris<\/jats:italic>.<\/jats:p>","DOI":"10.3389\/fnmol.2023.1091305","type":"journal-article","created":{"date-parts":[[2023,5,17]],"date-time":"2023-05-17T05:46:30Z","timestamp":1684302390000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":3,"title":["Transcriptome-wide selection and validation of a solid set of reference genes for gene expression studies in the cephalopod mollusk Octopus vulgaris"],"prefix":"10.3389","volume":"16","author":[{"given":"Pamela","family":"Imperadore","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefano","family":"Cagnin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vittoria","family":"Allegretti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caterina","family":"Millino","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesca","family":"Raffini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Graziano","family":"Fiorito","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giovanna","family":"Ponte","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2023,5,17]]},"reference":[{"key":"ref1","first-page":"91","article-title":"Effects of learning and memory on regional brain variations of cytochrome oxidase activity in Sepia officinalis","volume":"12","author":"Agin","year":"2000","journal-title":"Eur. 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Commun."},{"key":"ref5","doi-asserted-by":"publisher","first-page":"71","DOI":"10.1146\/annurev-animal-021419-083609","article-title":"Cephalopod biology: at the intersection between genomic and organismal novelties","volume":"8","author":"Albertin","year":"2020","journal-title":"Ann. Rev. Anim. Biosci."},{"key":"ref6","doi-asserted-by":"publisher","first-page":"e73792","DOI":"10.1371\/journal.pone.0073792","article-title":"Identification of appropriate reference genes for human mesenchymal cells during expansion and differentiation","volume":"8","author":"Amable","year":"2013","journal-title":"PLoS One"},{"key":"ref7","doi-asserted-by":"publisher","first-page":"3679","DOI":"10.1002\/ece3.7328","article-title":"Bipedal locomotion in Octopus vulgaris: a complementary observation and some preliminary considerations","volume":"11","author":"Amodio","year":"2021","journal-title":"Ecol. Evol."},{"key":"ref8","doi-asserted-by":"publisher","first-page":"329","DOI":"10.3389\/fnbeh.2018.00329","article-title":"The neurobiology of fear generalization","volume":"12","author":"Asok","year":"2019","journal-title":"Front. Behav. Neurosci."},{"key":"ref9","doi-asserted-by":"publisher","first-page":"1001","DOI":"10.3389\/fphys.2017.01001","article-title":"The gastric ganglion of Octopus vulgaris: preliminary characterization of gene- and Putative neurochemical-complexity, and the effect of Aggregata octopiana digestive tract infection on gene expression","volume":"8","author":"Baldascino","year":"2017","journal-title":"Front. Physiol."},{"key":"ref10","doi-asserted-by":"publisher","first-page":"253","DOI":"10.1007\/s13258-017-0588-6","article-title":"Transcriptome analysis of gene expression patterns during embryonic development in golden cuttlefish (Sepia esculenta)","volume":"40","author":"Bian","year":"2018","journal-title":"Genes Genom."},{"key":"ref11","doi-asserted-by":"publisher","first-page":"2114","DOI":"10.1093\/bioinformatics\/btu170","article-title":"Trimmomatic: a flexible trimmer for Illumina sequence data","volume":"30","author":"Bolger","year":"2014","journal-title":"Bioinformatics"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"605","DOI":"10.1016\/B978-012370509-9.00069-3","article-title":"Behavioral analysis of learning and memory in cephalopods","volume-title":"Learning and memory: a comprehensive reference","author":"Borrelli","year":"2008"},{"key":"ref13","doi-asserted-by":"publisher","first-page":"3746","DOI":"10.1073\/pnas.83.11.3746","article-title":"Predicting DNA duplex stability from the base sequence","volume":"83","author":"Breslauer","year":"1986","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"ref14","author":"Burbach","year":"2019"},{"key":"ref15","doi-asserted-by":"publisher","first-page":"23","DOI":"10.1677\/jme.0.0290023","article-title":"Quantification of mRNA using real-time reverse transcription PCR (RT-PCR): trends and problems","volume":"29","author":"Bustin","year":"2002","journal-title":"J. Mol. Endocrinol."},{"key":"ref16","doi-asserted-by":"publisher","first-page":"597","DOI":"10.1677\/jme.1.01755","article-title":"Quantitative real-time RT-PCR \u2013 a perspective","volume":"34","author":"Bustin","year":"2005","journal-title":"J. Mol. Endocrinol."},{"key":"ref17","doi-asserted-by":"publisher","first-page":"16279","DOI":"10.1073\/pnas.242597199","article-title":"Memory-specific temporal profiles of gene expression in the hippocampus","volume":"99","author":"Cavallaro","year":"2002","journal-title":"Proc. Natl. Acad. Sci."},{"key":"ref18","doi-asserted-by":"publisher","first-page":"97","DOI":"10.1016\/j.cub.2021.10.070","article-title":"Comparative brain structure and visual processing in octopus from different habitats","volume":"32","author":"Chung","year":"2022","journal-title":"Curr. Biol."},{"key":"ref19","doi-asserted-by":"publisher","first-page":"105846","DOI":"10.1016\/j.isci.2022.105846","article-title":"The brain structure and the neural network features of the diurnal cuttlefish Sepia plangon","volume":"26","author":"Chung","year":"2023","journal-title":"iScience"},{"key":"ref20","doi-asserted-by":"publisher","first-page":"1072382","DOI":"10.3389\/fcell.2022.1072382","article-title":"Deciphering regeneration through non-model animals: a century of experiments on cephalopod mollusks and an outlook at the future","volume":"10","author":"De Sio","year":"2023","journal-title":"Front. Cell Dev. Biol."},{"key":"ref21","doi-asserted-by":"publisher","first-page":"476","DOI":"10.1016\/j.tins.2009.05.008","article-title":"Animal consciousness: a synthetic approach","volume":"32","author":"Edelman","year":"2009","journal-title":"Trends Neurosci."},{"key":"ref22","doi-asserted-by":"publisher","first-page":"569","DOI":"10.1016\/j.tig.2013.05.010","article-title":"Human housekeeping genes, revisited","volume":"29","author":"Eisenberg","year":"2013","journal-title":"Trends Genet."},{"key":"ref001","year":"2010","journal-title":"Directive 2010\/63\/EU of the European Parliament and of the Council of 22 September 2010 on the protection of animals used for scientific purposes [Online]"},{"key":"ref23","doi-asserted-by":"publisher","first-page":"e0157821","DOI":"10.1371\/journal.pone.0157821","article-title":"Analyses of sox-B and sox-E family genes in the cephalopod Sepia officinalis: revealing the conserved and the unusual","volume":"11","author":"Focareta","year":"2016","journal-title":"PLoS One"},{"key":"ref24","doi-asserted-by":"publisher","first-page":"e109627","DOI":"10.1371\/journal.pone.0109627","article-title":"Characterization of homeobox genes reveals sophisticated regionalization of the central nervous system in the European cuttlefish Sepia officinalis","volume":"9","author":"Focareta","year":"2014","journal-title":"PLoS One"},{"key":"ref25","doi-asserted-by":"publisher","first-page":"45","DOI":"10.1007\/s12035-014-8842-2","article-title":"Identification and expression of acetylcholinesterase in Octopus vulgaris arm development and regeneration: a conserved role for ACHE?","volume":"52","author":"Fossati","year":"2015","journal-title":"Mol. Neurobiol."},{"key":"ref26","doi-asserted-by":"publisher","first-page":"93","DOI":"10.1016\/j.jembe.2013.02.015","article-title":"Octopus arm regeneration: role of acetylcholinesterase during morphological modification","volume":"447","author":"Fossati","year":"2013","journal-title":"J. Exp. Mar. Biol. Ecol."},{"key":"ref27","doi-asserted-by":"publisher","first-page":"e0188477","DOI":"10.1371\/journal.pone.0188477","article-title":"Identification and validation of reference genes for gene expression analysis in Aphidius gifuensis (hymenoptera: Aphidiidae)","volume":"12","author":"Gao","year":"2017","journal-title":"PLoS One"},{"key":"ref28","doi-asserted-by":"publisher","first-page":"57","DOI":"10.1016\/j.jip.2016.06.003","article-title":"Selection of reliable reference genes for RT-qPCR studies in Octopus vulgaris paralarvae during development and immune-stimulation","volume":"138","author":"Garc\u00eda-Fern\u00e1ndez","year":"2016","journal-title":"J. Invertebr. Pathol."},{"key":"ref29","doi-asserted-by":"publisher","first-page":"189","DOI":"10.2307\/1543389","article-title":"Gene expression in the squid giant axon: neurotransmitter modulation of RNA transfer from periaxonal glia to the axon","volume":"203","author":"Giuditta","year":"2002","journal-title":"Biol. Bull."},{"key":"ref30","doi-asserted-by":"publisher","first-page":"55","DOI":"10.1016\/0006-8993(71)90566-X","article-title":"Nuclear counts in the brain lobes of Octopus vulgaris as a function of body size","volume":"25","author":"Giuditta","year":"1971","journal-title":"Brain Res."},{"key":"ref31","doi-asserted-by":"publisher","first-page":"644","DOI":"10.1038\/nbt.1883","article-title":"Full-length transcriptome assembly from RNA-Seq data without a reference genome","volume":"29","author":"Grabherr","year":"2011","journal-title":"Nat. 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Bull."},{"key":"ref34","doi-asserted-by":"publisher","first-page":"112","DOI":"10.1186\/1471-2229-8-112","article-title":"Exploring valid reference genes for gene expression studies in Brachypodium distachyon by real-time PCR","volume":"8","author":"Hong","year":"2008","journal-title":"BMC Plant Biol."},{"key":"ref35","doi-asserted-by":"publisher","first-page":"1927","DOI":"10.1126\/science.1109616","article-title":"Underwater bipedal locomotion by octopuses in disguise","volume":"307","author":"Huffard","year":"2005","journal-title":"Science"},{"key":"ref36","doi-asserted-by":"publisher","first-page":"279","DOI":"10.1038\/sj.gene.6364190","article-title":"Real-time RT-PCR normalisation; strategies and considerations","volume":"6","author":"Huggett","year":"2005","journal-title":"Genes Immun."},{"key":"ref37","author":"Imperadore","year":"2017"},{"key":"ref38","doi-asserted-by":"publisher","first-page":"8408","DOI":"10.1038\/s41598-019-44849-1","article-title":"Identification of reliable reference genes for qRT-PCR in the ephemeral plant Arabidopsis pumila based on full-length transcriptome data","volume":"9","author":"Jin","year":"2019","journal-title":"Sci. Rep."},{"key":"ref39","volume-title":"Behavioral biology of Aplysia. A contribution to the comparative study of opisthobranch molluscs","author":"Kandel","year":"1979"},{"key":"ref40","doi-asserted-by":"publisher","first-page":"254","DOI":"10.1093\/icb\/43.2.254","article-title":"The squid-vibrio symbioses: from demes to Genes","volume":"43","author":"Kimbell","year":"2003","journal-title":"Integr. Comp. Biol."},{"key":"ref41","doi-asserted-by":"publisher","first-page":"191","DOI":"10.1016\/j.cell.2017.03.025","article-title":"Trade-off between transcriptome plasticity and genome evolution in cephalopods","volume":"169","author":"Liscovitch-Brauer","year":"2017","journal-title":"Cells"},{"key":"ref42","doi-asserted-by":"publisher","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using real-time quantitative PCR and the 2\u2212\u0394\u0394CT method","volume":"25","author":"Livak","year":"2001","journal-title":"Methods"},{"key":"ref43","doi-asserted-by":"publisher","first-page":"202","DOI":"10.1186\/s12915-022-01404-1","article-title":"Epigenetic machinery is functionally conserved in cephalopods","volume":"20","author":"Macchi","year":"2022","journal-title":"BMC Biol."},{"key":"ref44","doi-asserted-by":"crossref","first-page":"441","DOI":"10.1016\/B978-0-12-809324-5.21024-9","article-title":"Behavioral analysis of learning and memory in cephalopods","volume-title":"Learning and memory: A comprehensive reference","author":"Marini","year":"2017"},{"key":"ref45","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1016\/B978-012372540-0\/50005-4","article-title":"Chapter 4 - gene expression in learning and memory","volume-title":"Neurobiology of learning and memory","author":"Martinez","year":"2007"},{"key":"ref46","doi-asserted-by":"publisher","first-page":"306","DOI":"10.1037\/0735-7036.112.3.306","article-title":"How do octopuses use their arms?","volume":"112","author":"Mather","year":"1998","journal-title":"J. Comp. Psychol."},{"key":"ref47","doi-asserted-by":"publisher","first-page":"R64","DOI":"10.1186\/gb-2009-10-6-r64","article-title":"A novel and universal method for microRNA RT-qPCR data normalization","volume":"10","author":"Mestdagh","year":"2009","journal-title":"Genome Biol."},{"key":"ref48","doi-asserted-by":"publisher","first-page":"2199","DOI":"10.1093\/bioinformatics\/btv106","article-title":"Annocript: a flexible pipeline for the annotation of transcriptomes able to identify putative long noncoding RNAs","volume":"31","author":"Musacchia","year":"2015","journal-title":"Bioinformatics"},{"key":"ref49","doi-asserted-by":"publisher","first-page":"19","DOI":"10.1186\/s13227-015-0012-8","article-title":"The making of an octopus arm","volume":"6","author":"N\u00f6dl","year":"2015","journal-title":"EvoDevo"},{"key":"ref50","doi-asserted-by":"publisher","first-page":"1559","DOI":"10.1038\/nprot.2006.236","article-title":"Quantification of mRNA using real-time RT-PCR","volume":"1","author":"Nolan","year":"2006","journal-title":"Nat. Protoc."},{"key":"ref51","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1016\/B978-0-12-809633-8.90074-8","article-title":"Octopus\u2606","volume-title":"Encyclopedia of animal behavior","author":"O\u2019Brien","year":"2019"},{"key":"ref52","doi-asserted-by":"publisher","first-page":"241","DOI":"10.1111\/j.1469-185X.1972.tb00975.x","article-title":"Cephalopods and fish: the limits of convergence","volume":"47","author":"Packard","year":"1972","journal-title":"Biol. Rev."},{"key":"ref53","doi-asserted-by":"publisher","first-page":"539","DOI":"10.1242\/jeb.52.3.539","article-title":"Relative growth, nucleic acid content and cell numbers of the brain in Octopus vulgaris (Lamarck)","volume":"52","author":"Packard","year":"1970","journal-title":"J. Exp. Biol."},{"key":"ref54","author":"Petrosino","year":"2015"},{"key":"ref55","doi-asserted-by":"publisher","first-page":"116","DOI":"10.1186\/s12915-022-01303-5","article-title":"Identification of LINE retrotransposons and long non-coding RNAs expressed in the octopus brain","volume":"20","author":"Petrosino","year":"2022","journal-title":"BMC Biol."},{"key":"ref56","article-title":"De-novo assembly of the octopus transcriptome integrating custom and public sequencing data","volume-title":"BIOINFO","author":"Petrosino","year":"2015"},{"key":"ref57","doi-asserted-by":"publisher","first-page":"e45","DOI":"10.1093\/nar\/29.9.e45","article-title":"A new mathematical model for relative quantification in real-time RT\u2013PCR","volume":"29","author":"Pfaffl's","year":"2001","journal-title":"Nucleic Acids Res."},{"key":"ref58","doi-asserted-by":"publisher","first-page":"509","DOI":"10.1023\/B:BILE.0000019559.84305.47","article-title":"Determination of stable housekeeping genes, differentially regulated target genes and sample integrity: BestKeeper \u2013 excel-based tool using pair-wise correlations","volume":"26","author":"Pfaffl","year":"2004","journal-title":"Biotechnol. Lett."},{"key":"ref59","doi-asserted-by":"publisher","first-page":"787139","DOI":"10.3389\/fnsys.2021.787139","article-title":"Cephalopod behavior: from neural plasticity to consciousness","volume":"15","author":"Ponte","year":"2022","journal-title":"Front. Syst. Neurosci."},{"key":"ref60","doi-asserted-by":"publisher","first-page":"609","DOI":"10.1038\/s41597-022-01735-2","article-title":"De novo transcriptome reconstruction in aquacultured early life stages of the cephalopod Octopus vulgaris","volume":"9","author":"Prado-\u00c1lvarez","year":"2022","journal-title":"Sci. Data"},{"key":"ref61","doi-asserted-by":"publisher","first-page":"101","DOI":"10.1016\/j.mcp.2004.10.001","article-title":"Housekeeping gene variability in normal and cancerous colorectal, pancreatic, esophageal, gastric and hepatic tissues","volume":"19","author":"Rubie","year":"2005","journal-title":"Mol. Cell. 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Lett."},{"key":"ref65","doi-asserted-by":"publisher","first-page":"1297","DOI":"10.1002\/cne.23755","article-title":"The gyri of the octopus vertical lobe have distinct neurochemical identities","volume":"523","author":"Shigeno","year":"2015","journal-title":"J. Comp. Neurol."},{"key":"ref66","doi-asserted-by":"publisher","first-page":"33","DOI":"10.1186\/1471-2199-7-33","article-title":"Selection of housekeeping genes for gene expression studies in human reticulocytes using real-time PCR","volume":"7","author":"Silver","year":"2006","journal-title":"BMC Mol. Biol."},{"key":"ref67","doi-asserted-by":"publisher","first-page":"70","DOI":"10.1186\/1471-2199-10-70","article-title":"Selection and validation of a set of reliable reference genes for quantitative RT-PCR studies in the brain of the cephalopod mollusc Octopus vulgaris","volume":"10","author":"Sirakov","year":"2009","journal-title":"BMC Mol. Biol."},{"key":"ref68","doi-asserted-by":"publisher","first-page":"7392","DOI":"10.1038\/s41467-022-35198-1","article-title":"Cell type diversity in a developing octopus brain","volume":"13","author":"Styfhals","year":"2022","journal-title":"Nat. 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J."},{"key":"ref76","doi-asserted-by":"publisher","first-page":"75","DOI":"10.1007\/s11103-012-9885-2","article-title":"miRDeepFinder: a miRNA analysis tool for deep sequencing of plant small RNAs","volume":"80","author":"Xie","year":"2012","journal-title":"Plant Mol. Biol."},{"key":"ref77","doi-asserted-by":"publisher","first-page":"76","DOI":"10.1016\/j.etap.2018.04.010","article-title":"Selection of reference genes for quantitative real-time PCR in Octopus minor (Cephalopoda: Octopoda) under acute ammonia stress","volume":"60","author":"Xu","year":"2018","journal-title":"Environ. Toxicol. Pharmacol."},{"key":"ref78","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1242\/jcs.s2-75.297.1","article-title":"Memoirs: on the cytology of the neurons of cephalopods","author":"Young","year":"1932","journal-title":"J. Cell Sci."},{"key":"ref79","doi-asserted-by":"publisher","first-page":"229","DOI":"10.1111\/j.1469-7998.1963.tb01862.x","article-title":"The number and sizes of nerve cells in octopus","volume":"140","author":"Young","year":"1963","journal-title":"Proc. Zool. Soc. Lond"},{"key":"ref80","volume-title":"A model of the brain","author":"Young","year":"1964"},{"key":"ref81","volume-title":"Biostatistical analysis","author":"Zar","year":"1999"},{"key":"ref82","author":"Zarrella","year":"2011"},{"key":"ref83","doi-asserted-by":"publisher","first-page":"74","DOI":"10.1016\/j.conb.2015.06.012","article-title":"Learning and memory in Octopus vulgaris: a case of biological plasticity","volume":"35","author":"Zarrella","year":"2015","journal-title":"Curr. Opin. Neurobiol."},{"key":"ref84","doi-asserted-by":"publisher","first-page":"e40320","DOI":"10.1371\/journal.pone.0040320","article-title":"Transcriptome analysis of the Octopus vulgaris central nervous system","volume":"7","author":"Zhang","year":"2012","journal-title":"PLoS One"},{"key":"ref85","doi-asserted-by":"publisher","first-page":"271","DOI":"10.1007\/s00359-019-01332-6","article-title":"Motor control pathways in the nervous system of Octopus vulgaris arm","volume":"205","author":"Zullo","year":"2019","journal-title":"J. Comp. Physiol. A."},{"key":"ref86","doi-asserted-by":"publisher","first-page":"53","DOI":"10.3389\/fcell.2017.00053","article-title":"Molecular determinants of cephalopod muscles and their implication in muscle regeneration","volume":"5","author":"Zullo","year":"2017","journal-title":"Front. Cell Dev. 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