{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T14:04:47Z","timestamp":1785506687328,"version":"3.56.0"},"reference-count":60,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T00:00:00Z","timestamp":1785456000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004281","name":"Narodowe Centrum Nauki","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004281","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Mol. Neurosci."],"abstract":"<jats:p>\n                    Gelsolin (GSN) is a well-studied actin-binding protein that plays a crucial role in regulating the actin cytoskeleton. Previously, we showed that GSN mRNA is highly expressed in tissues of ectodermal origin and in neural crest cell (NCC) derivatives, suggesting a possible role for GSN in the development of melanocytes and the peripheral nervous system (PNS). Therefore, we explored gelsolin\u2019s role in NCC migration along dorsolateral and dorsoventral pathways using GSN-silencing constructs (sh\n                    <jats:italic>GSN<\/jats:italic>\n                    ) introduced into neural tubes (NTs). Additionally, we identified, for the first time, a secretory isoform of gelsolin (sGSN) in chicken embryos through bioinformatic analysis and neural tube (NT) and dorsal root ganglia (DRG) explant cultures. Using GSN-targeting antibodies to modulate sGSN activity\n                    <jats:italic>in vitro<\/jats:italic>\n                    , we demonstrated sGSN\u2019s contribution to cellular mechanisms that are important for generating components of the PNS from NCCs. Our findings revealed that sGSN is crucial for axonal outgrowth and the migration of Schwann cell precursors (SCPs) from DRG, though it does not affect NCC delamination and early migration from NT. Nonetheless, modulation of sGSN activity affected actin-cytoskeleton dynamics in both NCCs and DRG cells. We believe our studies will help elucidate GSN-mediated mechanisms in proper embryogenesis.\n                  <\/jats:p>","DOI":"10.3389\/fnmol.2026.1886575","type":"journal-article","created":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T13:18:25Z","timestamp":1785503905000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Contribution of gelsolin and its secretory isoform to the migratory properties of neural crest and DRG cells in the chicken embryo"],"prefix":"10.3389","volume":"19","author":[{"given":"Ewa","family":"Mr\u00f3wczy\u0144ska","sequence":"first","affiliation":[{"name":"Department of Cell Pathology, Faculty of Biotechnology, University of Wroc\u0142aw","place":["Wroc\u0142aw, Poland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonina Joanna","family":"Mazur","sequence":"additional","affiliation":[{"name":"Department of Cell Pathology, Faculty of Biotechnology, University of Wroc\u0142aw","place":["Wroc\u0142aw, Poland"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,7,31]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"169","DOI":"10.1016\/S0091-679X(08)00209-4","article-title":"In Situ hybridization analysis of chick embryos in whole-mount and tissue sections.","volume":"27","author":"Acloque","year":"2008","journal-title":"Methods Cell Biol."},{"key":"B2","doi-asserted-by":"publisher","first-page":"366","DOI":"10.1016\/j.cell.2009.07.049","article-title":"Schwann cell precursors from nerve innervation are a cellular origin of melanocytes in skin.","volume":"139","author":"Adameyko","year":"2009","journal-title":"Cell"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.21608\/zumj.2019.18972.1611","article-title":"Evaluation of plasma gelsolin level as a diagnostic biomarker in pediatric pneumonia.","author":"Al-Ghanay","year":"2019","journal-title":"Zagazig Univ. 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