{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,22]],"date-time":"2026-04-22T15:48:55Z","timestamp":1776872935099,"version":"3.51.2"},"reference-count":53,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2019,8,30]],"date-time":"2019-08-30T00:00:00Z","timestamp":1567123200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Nutr."],"DOI":"10.3389\/fnut.2019.00122","type":"journal-article","created":{"date-parts":[[2019,8,30]],"date-time":"2019-08-30T11:43:03Z","timestamp":1567165383000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":20,"title":["Down-Regulation of CYP79A1 Gene Through Antisense Approach Reduced the Cyanogenic Glycoside Dhurrin in [Sorghum bicolor (L.) Moench] to Improve Fodder Quality"],"prefix":"10.3389","volume":"6","author":[{"given":"Arun K.","family":"Pandey","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pusuluri","family":"Madhu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Basrur Venkatesh","family":"Bhat","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2019,8,30]]},"reference":[{"key":"B1","unstructured":"2018"},{"key":"B2","doi-asserted-by":"publisher","first-page":"903","DOI":"10.2135\/cropsci1987.0011183X002700050014x","article-title":"Seasonal variation in leaf hydrocyanic acid potential of low and high Dhurrin sorghum","volume":"27","author":"Haskins","year":"1987","journal-title":"Crop Sci."},{"key":"B3","doi-asserted-by":"publisher","first-page":"1022","DOI":"10.1104\/pp.63.6.1022","article-title":"Tissue distributions of dhurrin and enzymes involved in its metabolism in leaves of Sorghum bicolor","volume":"63","author":"Kojima","year":"1979","journal-title":"Plant Physiol."},{"key":"B4","doi-asserted-by":"publisher","first-page":"1661","DOI":"10.1104\/pp.115.4.1661","article-title":"Isolation and reconstitution of cytochrome P450ox and in vitro reconstitution of the entire biosynthetic pathway of the cyanogenic glucoside dhurrin from sorghum","volume":"115","author":"Kahn","year":"1997","journal-title":"Plant Physiol."},{"key":"B5","doi-asserted-by":"publisher","first-page":"177","DOI":"10.1006\/abbi.1995.0024","article-title":"The primary sequence of cytochrome P450tyr, the multifunctional N-Hydroxylase catalyzing the conversion of L-Tyrosine to p-Hydroxyphenyl acetaldehyde oxime in the biosynthesis of the cyanogenic glucoside dhurrin in Sorghum bicolor (L) Moench","volume":"323","author":"Koch","year":"1995","journal-title":"Arch. Biochem. Biophys."},{"key":"B6","doi-asserted-by":"publisher","first-page":"3506","DOI":"10.1074\/jbc.270.8.3506","article-title":"Cytochrome P450 is a multifunctional heme-thiolate enzyme, which catalyzes the conversion of l-tyrosine into p-hydroxyphenylacetaldehyde oxime in the biosynthesis of the cyanogene glucoside dhurrin in Sorghum bicolor (L.) Moench","volume":"270","author":"Sibbesen","year":"1995","journal-title":"J. Biol. Chem."},{"key":"B7","doi-asserted-by":"crossref","first-page":"393","DOI":"10.1023\/A:1005915507497","article-title":"Cloning of three A-type cytochromes P450, CYP71E1, CYP98, and CYP99 from Sorghum bicolor (L.) Moench by a PCR approach and identification by expression in Escherichia coli of CYP71E1 as a multifunctional cytochrome P450 in the biosynthesis of the cyanogenic glucoside dhurrin","volume":"36","author":"Bak","year":"1998","journal-title":"Plant Mol Biol."},{"key":"B8","doi-asserted-by":"publisher","first-page":"35483","DOI":"10.1074\/jbc.274.50.35483","article-title":"The UDP glucose-p-hydroxymandelo-nitrile-o-glucosyl transferase that catalyzes the last step in synthesis of the cyanogenic glucoside dhurrin from Sorghum bicolor","volume":"274","author":"Jones","year":"1999","journal-title":"J Biol Chem."},{"key":"B9","doi-asserted-by":"publisher","first-page":"54","DOI":"10.1111\/j.1467-7652.2011.00646.x","article-title":"A combined biochemical screen and TILLING approach identifies mutations in Sorghum bicolor L. Moench resulting in acyanogenic forage production","volume":"10","author":"Blomstedt","year":"2012","journal-title":"Plant Biotechnol J."},{"key":"B10","doi-asserted-by":"publisher","first-page":"155","DOI":"10.1146\/annurev-arplant-050213-040027","article-title":"Cyanogenic glycosides: synthesis, physiology, and phenotypic plasticity","volume":"29","author":"Gleadow","year":"2014","journal-title":"Annu Rev Plant Biol"},{"key":"B11","doi-asserted-by":"publisher","first-page":"1","DOI":"10.3835\/plantgenome2014.09.0048","article-title":"Natural variation in synthesis and catabolism genes influences dhurrin content in sorghum","volume":"8","author":"Hayes","year":"2015","journal-title":"Plant Genome."},{"key":"B12","doi-asserted-by":"publisher","first-page":"367","DOI":"10.1007\/s00425-003-1005-8","article-title":"Generation of cyanogen-free transgenic cassava","volume":"217","author":"Siritunga","year":"2003","journal-title":"Planta."},{"key":"B13","doi-asserted-by":"publisher","first-page":"1437","DOI":"10.1104\/pp.123.4.1437","article-title":"Transgenic tobacco and Arabidopsis plants expressing the two multifunctional sorghum cytochrome P450 enzymes, CYP79A1 and CYP71E1, are cyanogenic and accumulate metabolites derived from intermediates in dhurrin biosynthesis","volume":"123","author":"Bak","year":"2000","journal-title":"Plant Physiol."},{"key":"B14","doi-asserted-by":"publisher","first-page":"139","DOI":"10.1016\/j.copbio.2004.02.004","article-title":"RNA interference in crop plants","volume":"15","author":"Kusaba","year":"2004","journal-title":"Curr Opin Biotechnol."},{"key":"B15","doi-asserted-by":"publisher","first-page":"143","DOI":"10.1016\/S0031-9422(03)00261-9","article-title":"The in vitro substrate regiospecificity of recombinant UGT85B1, the cyanohydrin glucosyltransferase from Sorghum bicolor","volume":"64","author":"Hansen","year":"2003","journal-title":"Phytochemistry."},{"key":"B16","doi-asserted-by":"publisher","first-page":"2240","DOI":"10.2135\/cropsci2003.2240","article-title":"Improvement of forage quality by downregulation of maize O-methyltransferase","volume":"43","author":"He","year":"2003","journal-title":"Crop Sci."},{"key":"B17","doi-asserted-by":"publisher","first-page":"484","DOI":"10.1016\/j.ygeno.2013.10.008","article-title":"Role of antisense RNAs in evolution of yeast regulatory complexity","volume":"102","author":"Lin","year":"2013","journal-title":"Genomics."},{"key":"B18","doi-asserted-by":"publisher","first-page":"163","DOI":"10.5511\/plantbiotechnology.19.163","article-title":"An improved protocol for regeneration of Sorghum bicolor from isolated shoot apices","volume":"19","author":"Harshavardhan","year":"2002","journal-title":"Plant Biotechnol."},{"key":"B19","doi-asserted-by":"publisher","first-page":"323","DOI":"10.1007\/BF00233358","article-title":"Development of the particle inflow gun for DNA delivery to plant cells","volume":"11","author":"Finer","year":"1992","journal-title":"Plant Cell Rep."},{"key":"B20","doi-asserted-by":"publisher","first-page":"5466","DOI":"10.1073\/pnas.91.12.5466","article-title":"Extraordinarily polymorphic microsatellite DNA in barley: species diversity, chromosomal locations, and population dynamics","volume":"91","author":"Saghai","year":"1994","journal-title":"Proc Natl Acad Sci USA."},{"key":"B21","volume-title":"Molecular Cloning: A Laboratory Manual","author":"Sambrook","year":"1989"},{"key":"B22","doi-asserted-by":"publisher","first-page":"3231","DOI":"10.1074\/jbc.M706587200","article-title":"A functional genomics investigation of allelochemical biosynthesis in Sorghum bicolor root hairs","volume":"283","author":"Baerson","year":"2008","journal-title":"J Biol Chem."},{"key":"B23","doi-asserted-by":"publisher","first-page":"107","DOI":"10.1016\/S0308-8146(01)00313-2","article-title":"Total cyanide determination of plants and foods using the picrate and acid hydrolysis methods","volume":"77","author":"Haque","year":"2002","journal-title":"Food Chem."},{"key":"B24","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1007\/BF00048308","article-title":"Comparison of selective agents for use with the selectable marker gene bar in maize transformation","volume":"36","author":"Dennehey","year":"1994","journal-title":"Plant Cell Tissue Organ Cult."},{"key":"B25","doi-asserted-by":"publisher","first-page":"429","DOI":"10.1007\/s00299-008-0655-1","article-title":"Efficient, reproducible Agrobacterium-mediated transformation of sorghum using heat treatment of immature embryos","volume":"28","author":"Gurel","year":"2009","journal-title":"Plant Cell Rep."},{"key":"B26","doi-asserted-by":"publisher","first-page":"176","DOI":"10.1007\/s11627-013-9561-5","article-title":"Production and evaluation of transgenic sorghum for resistance to stem borer","volume":"50","author":"Visarada","year":"2014","journal-title":"In Vitro Cell Dev Biol Plant."},{"key":"B27","doi-asserted-by":"publisher","first-page":"513","DOI":"10.1007\/s00299-005-0947-7","article-title":"Development of transgenic sorghum for insect resistance against the spotted stem borer (Chilo partellus)","volume":"24","author":"Girijashankar","year":"2005","journal-title":"Plant Cell Rep."},{"key":"B28","doi-asserted-by":"publisher","first-page":"425","DOI":"10.1007\/s12041-015-0540-y","article-title":"In planta transformation of sorghum (Sorghum bicolor (L.) Moench) using TPS1 gene for enhancing tolerance to abiotic stresses","volume":"94","author":"Yellisetty","year":"2015","journal-title":"J Genet."},{"key":"B29","doi-asserted-by":"publisher","first-page":"719","DOI":"10.1016\/S0176-1617(98)80226-5","article-title":"In vitro morphogenesis of Sorghum bicolor (L.) Moench: efficient plant regeneration from shoot apices","volume":"153","author":"Zhong","year":"1998","journal-title":"J Plant Physiol."},{"key":"B30","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1023\/A:1024664817800","article-title":"Optimisation of transformation conditions and production of transgenic sorghum (Sorghum bicolor) via microparticle bombardment","volume":"75","author":"Tadesse","year":"2003","journal-title":"Plant Cell Tissue Organ Cult."},{"key":"B31","first-page":"75","article-title":"Production of Transgenic sorghum plants with related HVA1 gene","volume-title":"Sorghum Tissue Culture and Transformation","author":"Devi","year":"2004"},{"key":"B32","doi-asserted-by":"publisher","first-page":"13","DOI":"10.1007\/978-94-011-0357-2_2","article-title":"Strategies for variety-independent genetic transformation of important cereals, legumes and woody species utilizing particle bombardment","volume":"85","author":"Christou","year":"1995","journal-title":"Euphytica."},{"key":"B33","doi-asserted-by":"publisher","first-page":"81","DOI":"10.1046\/j.1467-7652.2003.00015.x","article-title":"Bioengineered \u2018golden\u2019indica rice cultivars with \u03b2-carotene metabolism in the endosperm with hygromycin and mannose selection systems","volume":"1","author":"Datta","year":"2003","journal-title":"Plant Biotechnol J."},{"key":"B34","doi-asserted-by":"publisher","first-page":"1101","DOI":"10.1038\/80310","article-title":"Field performance of transgenic elite commercial hybrid rice expressing Bacillus thuringiensis \u03b4-endotoxin","volume":"18","author":"Tu","year":"2000","journal-title":"Nat Biotechnol."},{"key":"B35","doi-asserted-by":"publisher","first-page":"125","DOI":"10.5511\/plantbiotechnology.18.125","article-title":"Transgenic IR72 with fused Bt gene cry1Ab\/cry1Ac from Bacillus thuringiensis is resistant against four lepidopteran species under field conditions","volume":"18","author":"Ye","year":"2001","journal-title":"Plant Biotechnol."},{"key":"B36","doi-asserted-by":"publisher","first-page":"463","DOI":"10.1023\/A:1008833324193","article-title":"Molecular characteristics of transgenic wheat and the effect on transgene expression","volume":"7","author":"Stoger","year":"1998","journal-title":"Transgenic Res."},{"key":"B37","doi-asserted-by":"publisher","first-page":"713","DOI":"10.1016\/S0168-9452(02)00165-6","article-title":"Expression of human lactoferrin in transgenic rice grains for the application in infant formula","volume":"163","author":"Nandi","year":"2002","journal-title":"Plant Sci"},{"key":"B38","doi-asserted-by":"publisher","first-page":"106","DOI":"10.1007\/PL00008669","article-title":"Alternative silencing effects involve distinct types of non-spreading cytosine methylation at a three-gene, single-copy transgenic locus in rice","volume":"263","author":"Fu","year":"2000","journal-title":"Mol Gen Genet."},{"key":"B39","doi-asserted-by":"publisher","first-page":"88","DOI":"10.1007\/s004250050538","article-title":"Transgene expression in rice engineered through particle bombardment: molecular factors controlling stable expression and transgene silencing","volume":"208","author":"Kohli","year":"1999","journal-title":"Planta."},{"key":"B40","doi-asserted-by":"publisher","first-page":"679","DOI":"10.1104\/pp.107.3.679","article-title":"How and why do plants inactivate homologous (trans) genes?","volume":"107","author":"Matzke","year":"1995","journal-title":"Plant Physiol."},{"key":"B41","doi-asserted-by":"publisher","first-page":"137","DOI":"10.1016\/j.jcs.2004.08.009","article-title":"Wheat transformation: current technology and applications to grain development and composition","volume":"41","author":"Jones","year":"2005","journal-title":"J Cereal Sci."},{"key":"B42","doi-asserted-by":"publisher","first-page":"129","DOI":"10.1046\/j.1365-313X.1998.00097.x","article-title":"Recurrent onset of epigenetic silencing in rice harboring a multi-copy transgene","volume":"14","author":"Kumpatla","year":"1998","journal-title":"Plant J."},{"key":"B43","doi-asserted-by":"publisher","first-page":"787","DOI":"10.1046\/j.1365-313X.1996.9060787.x","article-title":"Expression of single copies of a strongly expressed 35S transgene can be silenced post-transcriptionally","volume":"9","author":"Elmayan","year":"1996","journal-title":"Plant J."},{"key":"B44","doi-asserted-by":"publisher","first-page":"89","DOI":"10.1046\/j.1365-313X.1993.04010089.x","article-title":"Differences in DNA-methylation are associated with a paramutation phenomenon in transgenic petunia","volume":"4","author":"Meyer","year":"1993","journal-title":"Plant J."},{"key":"B45","doi-asserted-by":"publisher","first-page":"301","DOI":"10.2135\/cropsci1995.0011183X003500020001x","article-title":"Agrobacterium tumefaciens transformation of monocotyledons","volume":"35","author":"Smith","year":"1995","journal-title":"Crop Sci."},{"key":"B46","doi-asserted-by":"publisher","first-page":"619","DOI":"10.1007\/BF00046447","article-title":"Herbicide-resistant Indica rice plants from IRRI breeding line IR72 after PEG-mediated transformation of protoplasts","volume":"20","author":"Datta","year":"1992","journal-title":"Plant Mol Biol."},{"key":"B47","doi-asserted-by":"publisher","first-page":"2407","DOI":"10.1105\/tpc.11.12.2407","article-title":"Antisense inhibition of tomato fruit sucrose synthase decreases fruit setting and the sucrose unloading capacity of young fruit","volume":"11","author":"D'Aoust","year":"1999","journal-title":"Plant Cell."},{"key":"B48","doi-asserted-by":"publisher","first-page":"969","DOI":"10.1016\/S0168-9452(01)00505-2","article-title":"Repression of chilling-induced ACC accumulation in transgenic citrus by over-production of antisense 1-aminocyclopropane-1-carboxylate synthase RNA","volume":"161","author":"Wong","year":"2001","journal-title":"Plant Sci."},{"key":"B49","doi-asserted-by":"publisher","first-page":"235","DOI":"10.1038\/270235a0","article-title":"Changes in release rates of cyanide in relation to palatability of Sorghum to insects","volume":"270","author":"Woodhead","year":"1997","journal-title":"Nature."},{"key":"B50","doi-asserted-by":"publisher","first-page":"1826","DOI":"10.1126\/science.1062249","article-title":"Resistance to an herbivore through engineered cyanogenic glucoside synthesis","volume":"293","author":"Tattersall","year":"2001","journal-title":"Science."},{"key":"B51","doi-asserted-by":"publisher","first-page":"471","DOI":"10.1023\/A:1009634226797","article-title":"Multiple traits of agronomic importance in transgenic indica rice plants: analysis of transgene integration patterns, expression levels and stability","volume":"5","author":"Maqbool","year":"1999","journal-title":"Mol Breed."},{"key":"B52","doi-asserted-by":"publisher","first-page":"689","DOI":"10.1007\/s00425-004-1386-3","article-title":"Expression of Escherichia coli branching enzyme in caryopses of transgenic rice results in amylopectin with an increased degree of branching","volume":"220","author":"Kim","year":"2005","journal-title":"Planta."},{"key":"B53","doi-asserted-by":"publisher","first-page":"323","DOI":"10.1023\/A:1006412318311","article-title":"Transgene silencing in monocots","volume":"43","author":"Iyer","year":"2000","journal-title":"Plant Mol Biol."}],"container-title":["Frontiers in Nutrition"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/article\/10.3389\/fnut.2019.00122\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,8,30]],"date-time":"2019-08-30T11:43:07Z","timestamp":1567165387000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/article\/10.3389\/fnut.2019.00122\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,8,30]]},"references-count":53,"alternative-id":["10.3389\/fnut.2019.00122"],"URL":"https:\/\/doi.org\/10.3389\/fnut.2019.00122","relation":{},"ISSN":["2296-861X"],"issn-type":[{"value":"2296-861X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,8,30]]},"article-number":"122"}}