{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T15:50:41Z","timestamp":1785772241529,"version":"3.56.0"},"reference-count":55,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2021,4,1]],"date-time":"2021-04-01T00:00:00Z","timestamp":1617235200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Nutr."],"abstract":"<jats:p>During postharvest life, broccoli suffers from floret yellowing confining its economic and nutritional value. The objective of the present study was to explore the mechanisms employed by phytosulfokine \u03b1 (PSK\u03b1) at 150 nM for delaying floret yellowing in broccoli during storage at 4\u00b0C for 28 days. Our results showed that the higher endogenous accumulation of hydrogen sulfide (H<jats:sub>2<\/jats:sub>S) resulting from the higher gene expression and activities of <jats:sc>l<\/jats:sc>-cysteine desulfhydrase (<jats:italic>LCD<\/jats:italic>) and <jats:sc>d<\/jats:sc>-cysteine desulfhydrase (<jats:italic>DCD<\/jats:italic>) in broccoli floret treated with 150 nM PSK\u03b1 may serve as an endogenous signaling molecule for delaying senescence. Moreover, the suppressed ethylene biosynthesis in broccoli floret treated with 150 nM PSK\u03b1 might be ascribed to lower gene expression and activities of ACC synthase (<jats:italic>ACS<\/jats:italic>) and ACC oxidase (<jats:italic>ACO<\/jats:italic>). Furthermore, lower gene expression and activities of Mg<jats:sup>2+<\/jats:sup> dechelatase (<jats:italic>MDC<\/jats:italic>), pheophytinase (<jats:italic>PPH<\/jats:italic>), and pheophorbide a oxygenase (<jats:italic>PaO<\/jats:italic>) might be the reasons for the higher accumulation of chlorophyll in broccoli floret treated with 150 nM PSK\u03b1. Based on our findings, exogenous PSK\u03b1 application could be employed as signaling bioactive hormone for retarding floret yellowing of broccoli during storage at 4\u00b0C for 28 days.<\/jats:p>","DOI":"10.3389\/fnut.2021.609217","type":"journal-article","created":{"date-parts":[[2021,4,1]],"date-time":"2021-04-01T05:08:32Z","timestamp":1617253712000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":25,"title":["Delaying Broccoli Floret Yellowing by Phytosulfokine \u03b1 Application During Cold Storage"],"prefix":"10.3389","volume":"8","author":[{"given":"Morteza Soleimani","family":"Aghdam","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Majid","family":"Alikhani-Koupaei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raheleh","family":"Khademian","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2021,4,1]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"110965","DOI":"10.1016\/j.postharvbio.2019.110965","article-title":"UV-C hormesis in broccoli florets: preservation, phyto-compounds and gene expression","volume":"157","author":"Duarte-Sierra","year":"2019","journal-title":"Postharvest Biol Technol."},{"key":"B2","doi-asserted-by":"publisher","first-page":"98","DOI":"10.1016\/j.postharvbio.2016.01.007","article-title":"Exogenous sodium nitroprusside treatment of broccoli florets extends shelf life, enhances antioxidant enzyme activity, and inhibits chlorophyll-degradation","volume":"116","author":"Shi","year":"2016","journal-title":"Postharvest Biol Technol."},{"key":"B3","doi-asserted-by":"publisher","first-page":"33","DOI":"10.1016\/j.postharvbio.2016.04.021","article-title":"Effect of modified atmosphere packaging (MAP) on rachis quality of \u2018Red Globe\u2019 table grape variety","volume":"119","author":"Silva-Sanzana","year":"2016","journal-title":"Postharvest Biol Technol."},{"key":"B4","doi-asserted-by":"publisher","first-page":"136","DOI":"10.1016\/j.postharvbio.2017.03.017","article-title":"Effects of hydrogen sulfide on yellowing and energy metabolism in broccoli","volume":"129","author":"Li","year":"2017","journal-title":"Postharvest Biol Technol."},{"key":"B5","doi-asserted-by":"publisher","first-page":"133","DOI":"10.1016\/j.jprot.2013.05.014","article-title":"Proteomics and transcriptomics of broccoli subjected to exogenously supplied and transgenic senescence-induced cytokinin for amelioration of postharvest yellowing","volume":"93","author":"Liu","year":"2013","journal-title":"J Proteomics."},{"key":"B6","doi-asserted-by":"publisher","first-page":"87","DOI":"10.1016\/j.postharvbio.2019.04.019","article-title":"24-Epibrassinolide treatment regulates broccoli yellowing during shelf life","volume":"154","author":"Cai","year":"2019","journal-title":"Postharvest Biol Technol."},{"key":"B7","doi-asserted-by":"publisher","first-page":"524","DOI":"10.1016\/j.scienta.2018.09.009","article-title":"UV-B treatment controls chlorophyll degradation and related gene expression in broccoli (Brassica oleracea L. 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