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A total of 192 1-day-old chicks were evenly allocated to three experimental groups with eight replicates, a basal diet with 0 (Control), 3,000 mg\/kg (LMOA), 6,000 mg\/kg (HMOA) mixed organic acid. The tissue and serum samples were gathered on 21 and 42 d of the experiment. An increased (<jats:italic>P<\/jats:italic> &amp;lt; 0.05) concentration of IgA, D-lactate (D-LA), and interleukin-10 (IL-10) in the serum of broilers diets with HMOA was observed. The levels of total antioxidant capacity (T-AOC) and catalase activity (CAT) in serum were enhanced (<jats:italic>P<\/jats:italic> &amp;lt; 0.05) with dietary and mixed organic acid, respectively, and increased (<jats:italic>P<\/jats:italic> &amp;lt; 0.05) content of superoxide dismutase (SOD) and CAT in the duodenum of broilers diets with LMOA was noticed. Also, LMOA decreased (<jats:italic>P<\/jats:italic> &amp;lt; 0.05) the pH value of the duodenum and enhanced (<jats:italic>P<\/jats:italic> &amp;lt; 0.05) the amylase activity of the pancreas, the tight junction protein (mainly Claudin-1, Claudin-2, and ZO-1) in the duodenum of broilers fed with mixed organic acid were promoted (<jats:italic>P<\/jats:italic> &amp;lt; 0.05), and the LMOA group performed better in the small intestine. In cecum microbiota, LMOA and HMOA modulated the structure of microbiota and mainly reduced the relative abundance of <jats:italic>Escherichia coli<\/jats:italic>. In brief, dietary supplemented mixed organic acid improved the health status of broilers by promoting the immune function, enhancing the antioxidative characteristics and tight junction proteins expression as well as cecum microbiota. However, LMOA groups may be a better fit considering the comprehensive effects of experiments and economic costs.<\/jats:p>","DOI":"10.3389\/fnut.2021.673316","type":"journal-article","created":{"date-parts":[[2021,8,5]],"date-time":"2021-08-05T06:10:35Z","timestamp":1628143835000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":64,"title":["Effect of Dietary Supplementation With Mixed Organic Acids on Immune Function, Antioxidative Characteristics, Digestive Enzymes Activity, and Intestinal Health in Broiler Chickens"],"prefix":"10.3389","volume":"8","author":[{"given":"Jiayu","family":"Ma","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shad","family":"Mahfuz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiangshu","family":"Piao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2021,8,5]]},"reference":[{"key":"B1","volume-title":"The Evolving Threat of Antimicrobial Resistance. 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