{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,16]],"date-time":"2026-01-16T06:04:58Z","timestamp":1768543498191,"version":"3.49.0"},"reference-count":61,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2021,10,27]],"date-time":"2021-10-27T00:00:00Z","timestamp":1635292800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Nutr."],"abstract":"<jats:p>In this study, we successfully prepared scallop oil (SCO), which contains high levels of phospholipids (PL) and eicosapentaenoic acid (EPA), from the internal organs of the Japanese giant scallop (<jats:italic>Patinopecten yessoensis<\/jats:italic>), one of the most important underutilized fishery resources in Japan. The intake of SCO lowers the serum and liver cholesterol contents in mice; however, whether the fatty acids (FA) composition or PL of SCO exhibits any cholesterol-lowering effect remains unknown. To elucidate whether the cholesterol-lowering function is due to FA composition or PL of SCO, and investigate the cholesterol-lowering mechanism by SCO, in the present study, mice were fed SCO's PL fraction (SCO-PL), triglyceride (TG)-type oil with almost the same FA composition as SCO-PL, called SCO's TG fraction (SCO-TG), soybean oil (SOY-TG), and soybean's PL fraction (SOY-PL). Male C57BL\/6J mice (5-week-old) were fed high-fat and cholesterol diets containing 3% (w\/w) experimental oils (SOY-TG, SOY-PL, SCO-TG, and SCO-PL) for 28 days. The SCO-PL diet significantly decreased the serum and liver cholesterol contents compared with the SOY-TG diet, but the intake of SOY-PL and SCO-TG did not show this effect. This result indicated that the serum and liver cholesterol-lowering effect observed in the SCO intake group was due to the effect of SCO-PL. The cholesterol-lowering effect of SCO-PL was in part related to the promotion of liver cholesterol 7\u03b1-hydroxylase (CYP7A1) expression, which is the rate-limiting enzyme for bile acid synthesis. In contrast, the expression levels of the ileum farnesoid X receptor (<jats:italic>Fxr<\/jats:italic>) and fibroblast growth factor 15 (<jats:italic>Fgf15<\/jats:italic>), which inhibit the expression of liver CYP7A1, were significantly reduced in the SCO-PL group than the SOY-TG group. From these results, the increase in the liver CYP7A1 expression by dietary SCO-PL was in part through the reduction of the ileum <jats:italic>Fxr\/Fgf15<\/jats:italic> regulatory pathway. Therefore, this study showed that SCO-PL may be a health-promoting component as it lowers the serum and liver cholesterol contents by increasing the liver CYP7A1 expression, which is not seen in SOY-PL and SCO-TG.<\/jats:p>","DOI":"10.3389\/fnut.2021.761928","type":"journal-article","created":{"date-parts":[[2021,10,27]],"date-time":"2021-10-27T07:04:27Z","timestamp":1635318267000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":9,"title":["Dietary Phospholipids Prepared From Scallop Internal Organs Attenuate the Serum and Liver Cholesterol Contents by Enhancing the Expression of Cholesterol Hydroxylase in the Liver of Mice"],"prefix":"10.3389","volume":"8","author":[{"given":"Koki","family":"Sugimoto","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ryota","family":"Hosomi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Munehiro","family":"Yoshida","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kenji","family":"Fukunaga","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2021,10,27]]},"reference":[{"key":"B1","unstructured":"Cardiovascular diseases"},{"key":"B2","doi-asserted-by":"publisher","first-page":"e146","DOI":"10.1161\/cir.0000000000000485","article-title":"Heart disease and stroke statistics-2017 update: a report from the American Heart Association","volume":"135","author":"Benjamin","year":"2017","journal-title":"Circulation."},{"key":"B3","doi-asserted-by":"publisher","first-page":"1626","DOI":"10.1194\/jlr.M016246","article-title":"Dietary cholesterol exacerbates hepatic steatosis and inflammation in obese LDL receptor-deficient mice","volume":"52","author":"Subramanian","year":"2011","journal-title":"J Lipid Res."},{"key":"B4","doi-asserted-by":"publisher","first-page":"685","DOI":"10.1161\/atvbaha.107.157685","article-title":"Dietary cholesterol worsens adipose tissue macrophage accumulation and atherosclerosis in obese LDL receptor-deficient mice","volume":"28","author":"Subramanian","year":"2008","journal-title":"Arterioscler Thromb Vasc Biol."},{"key":"B5","doi-asserted-by":"publisher","first-page":"261","DOI":"10.1016\/j.cell.2004.11.043","article-title":"Hyperlipidemic effects of dietary saturated fats mediated through PGC-1beta coactivation of SREBP","volume":"120","author":"Lin","year":"2005","journal-title":"Cell."},{"key":"B6","doi-asserted-by":"publisher","first-page":"1387","DOI":"10.1001\/jama.281.15.1387","article-title":"A prospective study of egg consumption and risk of cardiovascular disease in men and women","volume":"281","author":"Hu","year":"1999","journal-title":"JAMA."},{"key":"B7","doi-asserted-by":"publisher","first-page":"911","DOI":"10.1161\/01.atv.12.8.911","article-title":"Effect of dietary fatty acids on serum lipids and lipoproteins. A meta-analysis of 27 trials","volume":"12","author":"Mensink","year":"1992","journal-title":"Arterioscler Thromb."},{"key":"B8","doi-asserted-by":"publisher","first-page":"1912","DOI":"10.3390\/nu10121912","article-title":"Dietary composition and cardiovascular risk: a mediator or a bystander?","volume":"10","author":"Korakas","year":"2018","journal-title":"Nutrients."},{"key":"B9","doi-asserted-by":"publisher","first-page":"e1","DOI":"10.1161\/cir.0000000000000510","article-title":"Dietary fats and cardiovascular disease: a presidential advisory from the American Heart Association","volume":"136","author":"Sacks","year":"2017","journal-title":"Circulation."},{"key":"B10","doi-asserted-by":"publisher","first-page":"502","DOI":"10.3945\/ajcn.2008.26285","article-title":"Saturated fat, carbohydrate, and cardiovascular disease","volume":"91","author":"Siri-Tarino","year":"2010","journal-title":"Am J Clin Nutr."},{"key":"B11","doi-asserted-by":"publisher","first-page":"217","DOI":"10.1002\/lipd.12018","article-title":"Egg-yolk sphingomyelin and phosphatidylcholine attenuate cholesterol absorption in Caco-2 cells","volume":"53","author":"Yang","year":"2018","journal-title":"Lipids."},{"key":"B12","doi-asserted-by":"publisher","first-page":"Cd003177","DOI":"10.1002\/14651858.CD003177.pub3","article-title":"Omega-3 fatty acids for the primary and secondary prevention of cardiovascular disease","volume":"7","author":"Abdelhamid","year":"2018","journal-title":"Cochrane Database Syst Rev."},{"key":"B13","doi-asserted-by":"publisher","first-page":"217","DOI":"10.1016\/0021-9150(81)90119-2","article-title":"The contrasting effects of a dietary soya lecithin product and corn oil on lipoprotein lipids in normolipidemic and familial hypercholesterolemic subjects","volume":"38","author":"Childs","year":"1981","journal-title":"Atherosclerosis."},{"key":"B14","doi-asserted-by":"publisher","first-page":"487","DOI":"10.1136\/gutjnl-2018-318155","article-title":"Milk polar lipids reduce lipid cardiovascular risk factors in overweight postmenopausal women: towards a gut sphingomyelin-cholesterol interplay","volume":"69","author":"Vors","year":"2020","journal-title":"Gut."},{"key":"B15","doi-asserted-by":"publisher","first-page":"213","DOI":"10.1186\/1476-511X-10-213","article-title":"Fish polar lipids retard atherosclerosis in rabbits by down-regulating PAF biosynthesis and up-regulating PAF catabolism","volume":"10","author":"Nasopoulou","year":"2011","journal-title":"Lipids Health Dis."},{"key":"B16","doi-asserted-by":"publisher","first-page":"116","DOI":"10.3390\/nu2020116","article-title":"Dietary phospholipids and intestinal cholesterol absorption","volume":"2","author":"Cohn","year":"2010","journal-title":"Nutrients."},{"key":"B17","doi-asserted-by":"crossref","first-page":"744","DOI":"10.1016\/S0022-2275(20)37345-4","article-title":"Mode of action of lecithin in suppressing cholesterol absorption","volume":"22","author":"Rampone","year":"1981","journal-title":"J Lipid Res."},{"key":"B18","doi-asserted-by":"publisher","first-page":"312","DOI":"10.1093\/ajcn\/84.1.312","article-title":"Dietary sphingolipids lower plasma cholesterol and triacylglycerol and prevent liver steatosis in APOE*3Leiden mice","volume":"84","author":"Duivenvoorden","year":"2006","journal-title":"Am J Clin Nutr."},{"key":"B19","doi-asserted-by":"publisher","first-page":"2358","DOI":"10.1093\/jn\/131.9.2358","article-title":"Egg phosphatidylcholine decreases the lymphatic absorption of cholesterol in rats","volume":"131","author":"Jiang","year":"2001","journal-title":"J Nutr."},{"key":"B20","doi-asserted-by":"publisher","first-page":"281","DOI":"10.1093\/ajcn\/17.5.281","article-title":"Quantitative effects of dietary fat on serum cholesterol in man","volume":"17","author":"Hegsted","year":"1965","journal-title":"Am J Clin Nutr."},{"key":"B21","doi-asserted-by":"publisher","first-page":"1335","DOI":"10.1007\/s10068-012-0176-0","article-title":"Combination effect of phospholipids and n-3 polyunsaturated fatty acids on rat cholesterol metabolism","volume":"21","author":"Hosomi","year":"2012","journal-title":"Food Sci Biotechnol."},{"key":"B22","doi-asserted-by":"publisher","first-page":"1449","DOI":"10.2331\/suisan.54.1449","article-title":"Eicosapentaenoic acid-rich triglycerides of scallop hepatopancreas","volume":"54","author":"Hayashi","year":"1988","journal-title":"Nippon Suisan Gakkaishi."},{"key":"B23","doi-asserted-by":"publisher","first-page":"1559","DOI":"10.2331\/suisan.52.1559","article-title":"Seasonal changes in eicosapentaenoic acid content of hepatopancreas of scallop Patinopecten yessoensis","volume":"52","author":"Hayashi","year":"1986","journal-title":"Nippon Suisan Gakkaishi"},{"key":"B24","doi-asserted-by":"publisher","first-page":"101","DOI":"10.14252\/foodsafetyfscj.2017031","article-title":"Distribution of diarrhetic shellfish toxins in mussels, scallops, and ascidian","volume":"6","author":"Matsushima","year":"2018","journal-title":"Food Safety."},{"key":"B25","doi-asserted-by":"publisher","first-page":"137","DOI":"10.2131\/fts.6.137","article-title":"Genotoxicity evaluation of oil prepared from the internal organs of the Japanese giant scallop (Patinopecten yessoensis)","volume":"6","author":"Sugimoto","year":"2019","journal-title":"Fund Toxicol Sci."},{"key":"B26","doi-asserted-by":"publisher","first-page":"691","DOI":"10.3390\/foods9060691","article-title":"Single and repeated dose 28-day and 13-week toxicity studies of oil prepared from the internal organs of the Japanese Giant Scallop (Patinopecten yessoensis) in mice","volume":"9","author":"Sugimoto","year":"2020","journal-title":"Foods."},{"key":"B27","doi-asserted-by":"publisher","first-page":"177","DOI":"10.2131\/fts.7.177","article-title":"Repeated 28-day and 13-week dose toxicity studies of oils prepared from the internal organs of the Japanese giant scallop (Patinopecten yessoensis) in rats","volume":"7","author":"Sugimoto","year":"2020","journal-title":"Fund Toxicol Sci."},{"key":"B28","doi-asserted-by":"publisher","first-page":"6727","DOI":"10.1002\/fsn3.1967","article-title":"Effects of dietary oils prepared from the internal organs of the Japanese giant scallop (Patinopecten yessoensis) on cholesterol metabolism in obese type-II diabetic KK-A(y) mice","volume":"8","author":"Sugimoto","year":"2020","journal-title":"Food Sci Nutr."},{"key":"B29","doi-asserted-by":"publisher","first-page":"965","DOI":"10.5650\/jos.ess21032","article-title":"Comparison of the cholesterol-lowering effect of scallop oil prepared from the internal organs of the Japanese Giant Scallop (Patinopecten yessoensis), fish oil, and krill oil in obese type II diabetic KK-A (y) mice","volume":"70","author":"Sugimoto","year":"2021","journal-title":"J Oleo Sci."},{"key":"B30","doi-asserted-by":"publisher","first-page":"151","DOI":"10.1038\/s41422-018-0123-6","article-title":"Blocking FSH inhibits hepatic cholesterol biosynthesis and reduces serum cholesterol","volume":"29","author":"Guo","year":"2019","journal-title":"Cell Res."},{"key":"B31","doi-asserted-by":"publisher","first-page":"53","DOI":"10.1007\/bf02558256","article-title":"Chromatographically homogeneous lecithin from egg phospholipids","volume":"42","author":"Singleton","year":"1965","journal-title":"J Am Oil Chem Soc."},{"key":"B32","doi-asserted-by":"publisher","first-page":"279","DOI":"10.1007\/s11745-016-4118-0","article-title":"Hypolipidemic effects of phospholipids (PL) containing n-3 polyunsaturated fatty acids (PUFA) are not dependent on esterification of n-3 PUFA to PL","volume":"51","author":"Fukunaga","year":"2016","journal-title":"Lipids."},{"key":"B33","doi-asserted-by":"publisher","first-page":"497","DOI":"10.3177\/jnsv.26.497","article-title":"Quantitative analysis of cholesterol in foods by gas-liquid chromatography","volume":"26","author":"Kaneda","year":"1980","journal-title":"J Nutr Sci Vitaminol (Tokyo)."},{"key":"B34","doi-asserted-by":"publisher","first-page":"494","DOI":"10.1007\/bf02531316","article-title":"Two dimensional then layer chromatographic separation of polar lipids and determination of phospholipids by phosphorus analysis of spots","volume":"5","author":"Rouser","year":"1970","journal-title":"Lipids."},{"key":"B35","doi-asserted-by":"publisher","first-page":"621","DOI":"10.1042\/bj0560621","article-title":"A. note on the estimation of sphingomyelin in nervous tissue","volume":"56","author":"Dawson","year":"1954","journal-title":"Biochem J."},{"key":"B36","doi-asserted-by":"publisher","first-page":"1939","DOI":"10.1093\/jn\/123.11.1939","article-title":"AIN-93 purified diets for laboratory rodents: final report of the American Institute of Nutrition ad hoc writing committee on the reformulation of the AIN-76A rodent diet","volume":"123","author":"Reeves","year":"1993","journal-title":"J Nutr."},{"key":"B37","doi-asserted-by":"publisher","first-page":"911","DOI":"10.1139\/o59-099","article-title":"A rapid method of total lipid extraction and purification","volume":"37","author":"Bligh","year":"1959","journal-title":"Can J Biochem Physiol."},{"key":"B38","doi-asserted-by":"publisher","first-page":"680","DOI":"10.1038\/227680a0","article-title":"Cleavage of structural proteins during the assembly of the head of bacteriophage T4","volume":"227","author":"Laemmli","year":"1970","journal-title":"Nature."},{"key":"B39","doi-asserted-by":"publisher","first-page":"2403","DOI":"10.1093\/jn\/113.12.2403","article-title":"The contrasting effect of dietary phosphatidylethanolamine and phosphatidylcholine on serum lipoproteins and liver lipids in rats","volume":"113","author":"Imaizumi","year":"1983","journal-title":"J Nutr."},{"key":"B40","doi-asserted-by":"publisher","first-page":"295","DOI":"10.4162\/nrp.2019.13.4.295","article-title":"Egg phospholipids exert an inhibitory effect on intestinal cholesterol absorption in mice","volume":"13","author":"Lee","year":"2019","journal-title":"Nutr Res Pract."},{"key":"B41","doi-asserted-by":"publisher","first-page":"36","DOI":"10.1194\/jlr.M400199-JLR200","article-title":"Interaction of cholesterol with sphingosine: physicochemical characterization and impact on intestinal absorption","volume":"46","author":"Garmy","year":"2005","journal-title":"J Lipid Res."},{"key":"B42","doi-asserted-by":"publisher","first-page":"2611","DOI":"10.1093\/jn\/134.10.2611","article-title":"Milk sphingomyelin is more effective than egg sphingomyelin in inhibiting intestinal absorption of cholesterol and fat in rats","volume":"134","author":"Noh","year":"2004","journal-title":"J Nutr."},{"key":"B43","doi-asserted-by":"publisher","first-page":"66","DOI":"10.1016\/s0163-7827(01)00020-0","article-title":"Cholesterol interactions with phospholipids in membranes","volume":"41","author":"Ohvo-Rekil\u00e4","year":"2002","journal-title":"Prog Lipid Res."},{"key":"B44","doi-asserted-by":"publisher","first-page":"13891","DOI":"10.1038\/s41598-020-70888-0","article-title":"Dietary ceramide 2-aminoethylphosphonate, a marine sphingophosphonolipid, improves skin barrier function in hairless mice","volume":"10","author":"Tomonaga","year":"2020","journal-title":"Sci Rep."},{"key":"B45","doi-asserted-by":"crossref","first-page":"831","DOI":"10.1136\/gut.14.10.790","article-title":"Proceedings: the effect of chemically defined diets on the faecal flora and faecal steroids","volume":"14","author":"Crowther","year":"1973","journal-title":"Gut."},{"key":"B46","doi-asserted-by":"publisher","first-page":"518","DOI":"10.1016\/j.jnutbio.2009.02.010","article-title":"Polyunsaturated fatty acids down-regulate in vitro expression of the key intestinal cholesterol absorption protein NPC1L1: no effect of monounsaturated nor saturated fatty acids","volume":"21","author":"Alvaro","year":"2010","journal-title":"J Nutr Biochem."},{"key":"B47","doi-asserted-by":"publisher","first-page":"996","DOI":"10.1002\/hep.24107","article-title":"Overexpression of cholesterol 7\u03b1-hydroxylase promotes hepatic bile acid synthesis and secretion and maintains cholesterol homeostasis","volume":"53","author":"Li","year":"2011","journal-title":"Hepatology."},{"key":"B48","doi-asserted-by":"publisher","first-page":"G878","DOI":"10.1152\/ajpgi.2001.281.4.G878","article-title":"Effects of CYP7A1 overexpression on cholesterol and bile acid homeostasis","volume":"281","author":"Pandak","year":"2001","journal-title":"Am J Physiol Gastrointest Liver Physiol."},{"key":"B49","doi-asserted-by":"publisher","first-page":"2065","DOI":"10.1210\/me.2001-0194","article-title":"The small heterodimer partner interacts with the liver X receptor alpha and represses its transcriptional activity","volume":"16","author":"Brendel","year":"2002","journal-title":"Mol Endocrinol."},{"key":"B50","doi-asserted-by":"publisher","first-page":"507","DOI":"10.1016\/s1097-2765(00)00050-2","article-title":"Molecular basis for feedback regulation of bile acid synthesis by nuclear receptors","volume":"6","author":"Lu","year":"2000","journal-title":"Mol Cell."},{"key":"B51","doi-asserted-by":"crossref","first-page":"533","DOI":"10.1016\/S0022-2275(20)31482-6","article-title":"Regulation of cholesterol-7alpha-hydroxylase: BAREly missing a SHP","volume":"43","author":"Davis","year":"2002","journal-title":"J Lipid Res."},{"key":"B52","doi-asserted-by":"publisher","first-page":"21805","DOI":"10.1074\/jbc.C000275200","article-title":"Bile acid induction of cytokine expression by macrophages correlates with repression of hepatic cholesterol 7alpha-hydroxylase","volume":"275","author":"Miyake","year":"2000","journal-title":"J Biol Chem."},{"key":"B53","doi-asserted-by":"publisher","first-page":"G74","DOI":"10.1152\/ajpgi.00258.2004","article-title":"Mechanism of rifampicin and pregnane X receptor inhibition of human cholesterol 7 alpha-hydroxylase gene transcription","volume":"288","author":"Li","year":"2005","journal-title":"Am J Physiol Gastrointest Liver Physiol."},{"key":"B54","doi-asserted-by":"publisher","first-page":"G404","DOI":"10.1152\/ajpgi.00188.2018","article-title":"Defective FXR-FGF15 signaling and bile acid homeostasis in cystic fibrosis mice can be restored by the laxative polyethylene glycol","volume":"316","author":"Bertolini","year":"2019","journal-title":"Am J Physiol Gastrointest Liver Physiol."},{"key":"B55","doi-asserted-by":"publisher","first-page":"5339","DOI":"10.1021\/acs.jafc.9b08296","article-title":"Eicosapentaenoic acid-enriched phosphoethanolamine plasmalogens alleviated atherosclerosis by remodeling gut microbiota to regulate bile acid metabolism in LDLR(-\/-) mice","volume":"68","author":"Ding","year":"2020","journal-title":"J Agric Food Chem."},{"key":"B56","doi-asserted-by":"publisher","first-page":"217","DOI":"10.1016\/j.cmet.2005.09.001","article-title":"Fibroblast growth factor 15 functions as an enterohepatic signal to regulate bile acid homeostasis","volume":"2","author":"Inagaki","year":"2005","journal-title":"Cell Metab."},{"key":"B57","doi-asserted-by":"publisher","first-page":"225","DOI":"10.1016\/j.cmet.2013.01.003","article-title":"Gut microbiota regulates bile acid metabolism by reducing the levels of tauro-beta-muricholic acid, a naturally occurring FXR antagonist","volume":"17","author":"Sayin","year":"2013","journal-title":"Cell Metab."},{"key":"B58","doi-asserted-by":"publisher","first-page":"1509","DOI":"10.1194\/jlr.R900007-JLR200","article-title":"Bile acids as regulatory molecules","volume":"50","author":"Hylemon","year":"2009","journal-title":"J Lipid Res."},{"key":"B59","doi-asserted-by":"publisher","first-page":"1077","DOI":"10.5650\/jos.ess20096","article-title":"Oligonol, a low-molecular weight polyphenol extracted from lychee fruit, modulates cholesterol metabolism in rats within a short period","volume":"69","author":"Hayashi","year":"2020","journal-title":"J Oleo Sci."},{"key":"B60","doi-asserted-by":"publisher","first-page":"41","DOI":"10.1016\/j.cmet.2016.05.005","article-title":"Intestinal crosstalk between bile acids and microbiota and its impact on host metabolism","volume":"24","author":"Wahlstr\u00f6m","year":"2016","journal-title":"Cell Metab."},{"key":"B61","doi-asserted-by":"publisher","first-page":"103824","DOI":"10.1016\/j.jff.2020.103824","article-title":"EPA-enriched ethanolamine plasmalogen alleviates atherosclerosis via mediating bile acids metabolism","volume":"66","author":"Ding","year":"2020","journal-title":"J Funct Foods."}],"container-title":["Frontiers in Nutrition"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fnut.2021.761928\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,10,27]],"date-time":"2021-10-27T07:04:31Z","timestamp":1635318271000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fnut.2021.761928\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,10,27]]},"references-count":61,"alternative-id":["10.3389\/fnut.2021.761928"],"URL":"https:\/\/doi.org\/10.3389\/fnut.2021.761928","relation":{},"ISSN":["2296-861X"],"issn-type":[{"value":"2296-861X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,10,27]]},"article-number":"761928"}}