{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,15]],"date-time":"2026-08-15T04:44:06Z","timestamp":1786769046590,"version":"build-2736575974"},"reference-count":54,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,1,18]],"date-time":"2022-01-18T00:00:00Z","timestamp":1642464000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Nutr."],"abstract":"<jats:p>The aim of this study was to evaluate the effect of <jats:italic>Hermetia illucens<\/jats:italic> larvae meal (HI) on the growth performance and intestinal barrier function of weaned pigs. To achieve this, 72 weaned pigs [28-day-old, 8.44 \u00b1 0.04 kg body weight (BW)] were randomly assigned to three dietary treatments: basal diet (negative control, NC), zinc oxide-supplemented diet (positive control, PC), and HI-supplemented diet [100% replacement of fishmeal (FM), HI], for 28 days in the presence of enterotoxigenic <jats:italic>Escherichia coli<\/jats:italic> (ETEC). The results showed that HI and PC increased (<jats:italic>p<\/jats:italic> &amp;lt; 0.05) the average daily gain (ADG) and average daily feed intake (ADFI) of weaned pigs from day 1 to 14, and decreased diarrhea incidence from day 1 to 28. Additionally, HI increased (<jats:italic>p<\/jats:italic> &amp;lt; 0.05) claudin-1, occludin, mucin-1 (<jats:italic>MUC-1<\/jats:italic>), and MUC-2 expression, goblet cell number, and secretory immunoglobulin A (sIgA) concentration in the intestine of weaned pigs. Compared with NC, HI downregulated (<jats:italic>p<\/jats:italic> &amp;lt; 0.05) interleukin-1\u03b2 (<jats:italic>IL-1<\/jats:italic>\u03b2) and <jats:italic>IL-8<\/jats:italic> expression, and upregulated <jats:italic>IL-10<\/jats:italic>, transforming growth factor-\u03b2 (<jats:italic>TGF-<\/jats:italic>\u03b2), antimicrobial peptide [porcine \u03b2 defensin 1 (<jats:italic>pBD1<\/jats:italic>), <jats:italic>pBD2<\/jats:italic>, protegrin 1-5 (<jats:italic>PG1-5<\/jats:italic>)] expression in the jejunum or ileum. Moreover, HI decreased (<jats:italic>p<\/jats:italic> &amp;lt; 0.05) toll-like receptor 2 (TLR2), phosphorylated nuclear factor-\u03baB (p-NF-\u03baB), and phosphorylated mitogen-activated protein kinase (p-MAPK) expression, and increased sirtuin 1 (SIRT1) expression in the ileum. Additionally, HI increased histone deacetylase 3 (HDAC3) expression and acetylation of histone 3 lysine 27 (acH3k27) in the ileum. Furthermore, HI positively influenced the intestinal microbiota composition and diversity of weaned pigs and increased (<jats:italic>p<\/jats:italic> &amp;lt; 0.05) butyrate and valerate concentrations. Overall, dietary HI improved growth performance and intestinal barrier function, as well as regulated histone acetylation and TLR2-NF-\u03baB\/MAPK signaling pathways in weaned pigs.<\/jats:p>","DOI":"10.3389\/fnut.2021.812011","type":"journal-article","created":{"date-parts":[[2022,1,18]],"date-time":"2022-01-18T15:25:52Z","timestamp":1642519552000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":26,"title":["Dietary Hermetia illucens Larvae Meal Improves Growth Performance and Intestinal Barrier Function of Weaned Pigs Under the Environment of Enterotoxigenic Escherichia coli K88"],"prefix":"10.3389","volume":"8","author":[{"given":"Qingsong","family":"Tang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E.","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhikang","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingfei","family":"Xiao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuting","family":"Cao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shenglan","family":"Hu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiwen","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yunxia","family":"Xiong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zongyong","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fengying","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Geling","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongbo","family":"Yi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2022,1,18]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"45","DOI":"10.2527\/af.2015-0019","article-title":"Insects: a protein-rich feed ingredient in pig and poultry diets","volume":"5","author":"Veldkamp","year":"2015","journal-title":"Anim Front"},{"key":"B2","doi-asserted-by":"publisher","first-page":"105","DOI":"10.3920\/JIFF2016.0055","article-title":"Nutritional value of the black soldier fly (Hermetia illucens L.) and its suitability as animal feed - 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