{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T09:19:34Z","timestamp":1787908774681,"version":"build-2784847793"},"reference-count":40,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,11,4]],"date-time":"2022-11-04T00:00:00Z","timestamp":1667520000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Nutr."],"abstract":"<jats:p>Tannic acid (TA) has received widespread attention for its beneficial biological function with antioxidant capacity. This study investigated the protective role of TA on the intestinal antioxidant capacity and intestinal barrier in weaned piglets and porcine intestinal epithelial cells (IPEC-J2). A total of 18 weaned piglets were randomly allocated into two groups (<jats:italic>n<\/jats:italic> = 9) and fed with a basal diet (control, CON) and a basal diet containing 1,000 mg\/kg TA for two weeks. The <jats:italic>in vivo<\/jats:italic> results showed that treatment with TA increased both glutathione peroxidase (GSH-PX) activity and the protein expression of ZO-1 in the jejunum of weaned piglets, and reduced the level of malondialdehyde (MDA) in the serum and the mRNA and protein expression of Keap1 in the jejunum of weaned piglets. Furthermore, <jats:italic>in vitro<\/jats:italic> results indicated that TA treatment effectively alleviated tert-butyl hydroperoxide (TBH)-induced oxidative stress in IPEC-J2 cells, improved the antioxidant capacity by elevating the cell redox state and activating the Nrf2 pathway, and improved the intestinal barrier by upregulating the mRNA and protein expression of intestinal tight junction proteins and increasing the transepithelial electrical resistance (TEER) value. In conclusion, these results confirmed that TA relieves oxidative injury and improves intestinal barrier function and intestinal antioxidant capacity by activating the Nrf2 signaling pathway. These findings suggest that TA has the potential application in alleviating oxidative stress in the intestine of weaned piglets.<\/jats:p>","DOI":"10.3389\/fnut.2022.1012207","type":"journal-article","created":{"date-parts":[[2022,11,4]],"date-time":"2022-11-04T07:02:20Z","timestamp":1667545340000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":19,"title":["Tannic acid attenuates intestinal oxidative damage by improving antioxidant capacity and intestinal barrier in weaned piglets and IPEC-J2 cells"],"prefix":"10.3389","volume":"9","author":[{"given":"Meiwei","family":"Wang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huijun","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lanmei","family":"Yin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huansheng","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chiqing","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiankun","family":"Zheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shanping","family":"He","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2022,11,4]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"31","DOI":"10.1207\/s15327604jaws0201_3","article-title":"Segregated early weaning and welfare of piglets","volume":"2","author":"Robert","year":"1999","journal-title":"J Appl Anim Welf Sci."},{"key":"B2","doi-asserted-by":"publisher","first-page":"313","DOI":"10.1016\/j.aninu.2017.06.003","article-title":"Weaning stress and gastrointestinal barrier development: implications for lifelong gut health in pigs","volume":"3","author":"Moeser","year":"2017","journal-title":"Anim Nutr."},{"key":"B3","doi-asserted-by":"publisher","first-page":"584","DOI":"10.1016\/j.nut.2013.10.018","article-title":"Regulation of an antioxidant blend on intestinal redox status and major microbiota in early weaned piglets","volume":"30","author":"Xu","year":"2014","journal-title":"Nutrition."},{"key":"B4","doi-asserted-by":"publisher","first-page":"612","DOI":"10.2527\/jas.2013-6986","article-title":"Development of an antioxidant system after early weaning in piglets","volume":"92","author":"Yin","year":"2014","journal-title":"J Anim Sci."},{"key":"B5","first-page":"132","article-title":"Oxidative stress-mediated signaling pathways: a review","volume":"11","author":"Yin","year":"2013","journal-title":"J Food Agric Environ."},{"key":"B6","doi-asserted-by":"publisher","first-page":"139","DOI":"10.1186\/s12944-018-0788-3","article-title":"Effects of plant essential oil supplementation on growth performance, immune function and antioxidant activities in weaned pigs","volume":"17","author":"Su","year":"2018","journal-title":"Lipids Health Dis."},{"key":"B7","doi-asserted-by":"publisher","first-page":"240","DOI":"10.1080\/10495398.2019.1683022","article-title":"Effects of dietary resveratrol supplementation on immunity, antioxidative capacity and intestinal barrier function in weaning piglets","volume":"32","author":"Chen","year":"2021","journal-title":"Anim Biotechnol."},{"key":"B8","doi-asserted-by":"publisher","first-page":"13146","DOI":"10.1021\/acs.jafc.9b08298","article-title":"How to stabilize omega-3 polyunsaturated fatty acids (PUFAs) in an animal feeding study?","volume":"68","author":"Zhang","year":"2020","journal-title":"J Agric Food Chem"},{"key":"B9","doi-asserted-by":"publisher","first-page":"19","DOI":"10.3390\/antiox5020019","article-title":"Effects of tannic acid on lipid and protein oxidation, color, and volatiles of raw and cooked chicken breast meat during storage","volume":"5","author":"Al-Hijazeen","year":"2016","journal-title":"Antioxidants."},{"key":"B10","doi-asserted-by":"publisher","first-page":"43","DOI":"10.1016\/j.arabjc.2009.12.008","article-title":"Radical scavenging and antioxidant activity of tannic acid","volume":"3","author":"Gulcin","year":"2010","journal-title":"Arab J Chem."},{"key":"B11","doi-asserted-by":"publisher","first-page":"603","DOI":"10.1002\/tox.22549","article-title":"A natural antioxidant, tannic acid mitigates iron-overload induced hepatotoxicity in Swiss albino mice through ROS regulation","volume":"33","author":"Basu","year":"2018","journal-title":"Environ Toxicol."},{"key":"B12","doi-asserted-by":"publisher","first-page":"142","DOI":"10.1016\/S0304-4165(99)00117-8","article-title":"Polyphenol tannic acid inhibits hydroxyl radical formation from Fenton reaction by complexing ferrous ions","volume":"1472","author":"Lopes","year":"1999","journal-title":"Biochim Biophys Acta."},{"key":"B13","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.abb.2005.02.016","article-title":"The antioxidant effect of tannic acid on the in vitro copper-mediated formation of free radicals","volume":"437","author":"Andrade","year":"2005","journal-title":"Arch Biochem Biophys."},{"key":"B14","doi-asserted-by":"publisher","first-page":"757","DOI":"10.3390\/ani10050757","article-title":"Effects of hydrolysable tannins as zinc oxide substitutes on antioxidant status, immune function, intestinal morphology, and digestive enzyme activities in weaned piglets","volume":"10","author":"Liu","year":"2020","journal-title":"Animals."},{"key":"B15","doi-asserted-by":"publisher","first-page":"31988","DOI":"10.1039\/C9RA04943F","article-title":"Tannic acid modulates intestinal barrier functions associated with intestinal morphology, antioxidative activity, and intestinal tight junction in a diquat-induced mouse model","volume":"9","author":"Wang","year":"2019","journal-title":"RSC Adv."},{"key":"B16","doi-asserted-by":"publisher","first-page":"skaa112","DOI":"10.1093\/jas\/skaa112","article-title":"Effects of dietary microencapsulated tannic acid supplementation on the growth performance, intestinal morphology, and intestinal microbiota in weaning piglets","volume":"98","author":"Wang","year":"2020","journal-title":"J Anim Sci."},{"key":"B17","doi-asserted-by":"publisher","first-page":"136","DOI":"10.1038\/s41467-021-27762-y","article-title":"Microbial enzymes induce colitis by reactivating triclosan in the mouse gastrointestinal tract","volume":"13","author":"Zhang","year":"2022","journal-title":"Nat Commun."},{"key":"B18","doi-asserted-by":"publisher","first-page":"12412","DOI":"10.1021\/acs.jafc.8b03574","article-title":"Structurally different flavonoid subclasses attenuate high-fat and high-fructose diet induced metabolic syndrome in rats","volume":"66","author":"Zhang","year":"2018","journal-title":"J Agric Food Chem."},{"key":"B19","doi-asserted-by":"publisher","first-page":"549","DOI":"10.1002\/ejlt.200700145","article-title":"Tannins: the new natural antioxidants?","volume":"109","author":"Amarowicz","year":"2007","journal-title":"Eur J Lipid Sci Technol."},{"key":"B20","doi-asserted-by":"publisher","first-page":"137","DOI":"10.1016\/j.aninu.2017.09.004","article-title":"Potential and challenges of tannins as an alternative to in-feed antibiotics for farm animal production","volume":"4","author":"Huang","year":"2018","journal-title":"Anim Nutr."},{"key":"B21","first-page":"728","article-title":"Antioxidant properties of tannic acid and its inhibitory effects on paraquat-induced oxidative stress in mice","volume":"15","author":"Choi","year":"2006","journal-title":"Food Sci Biotechnol."},{"key":"B22","doi-asserted-by":"publisher","first-page":"119","DOI":"10.1002\/jbt.20061","article-title":"Response of the antioxidant Defense system to tert-butyl hydroperoxide and hydrogen peroxide in a human hepatoma cell line (HepG2)","volume":"19","author":"Alia","year":"2005","journal-title":"J Biochem Mol Toxicol."},{"key":"B23","doi-asserted-by":"publisher","first-page":"583","DOI":"10.1152\/physrev.00011.2005","article-title":"Heme oxygenase-1\/carbon monoxide: from basic science to therapeutic applications","volume":"86","author":"Ryter","year":"2006","journal-title":"Physiol Rev."},{"key":"B24","doi-asserted-by":"publisher","first-page":"13291","DOI":"10.1074\/jbc.R900010200","article-title":"The Nrf2-antioxidant response element signaling pathway and its activation by oxidative stress","volume":"284","author":"Nguyen","year":"2009","journal-title":"J Biol Chem."},{"key":"B25","doi-asserted-by":"publisher","first-page":"89","DOI":"10.1146\/annurev.pharmtox.46.120604.141046","article-title":"Cell survival responses to environmental stresses via the Keap1-Nrf2-ARE pathway","volume":"47","author":"Kensler","year":"2007","journal-title":"Annu Rev Pharmacol Toxicol."},{"key":"B26","doi-asserted-by":"publisher","first-page":"110047","DOI":"10.1016\/j.biopha.2020.110047","article-title":"Tannic acid ameliorates arsenic trioxide-induced nephrotoxicity, contribution of NF-kappa B and Nrf2 pathways","volume":"126","author":"Jin","year":"2020","journal-title":"Biomed Pharmacother."},{"key":"B27","doi-asserted-by":"publisher","first-page":"2870","DOI":"10.1007\/s12035-020-01924-3","article-title":"Tannic acid provides neuroprotective effects against traumatic brain injury through the PGC-1 alpha\/Nrf2\/HO-1 pathway","volume":"57","author":"Salman","year":"2020","journal-title":"Mol Neurobiol."},{"key":"B28","doi-asserted-by":"publisher","first-page":"23","DOI":"10.3389\/fphar.2018.00023","article-title":"Tannins enriched fraction of emblica officinalis fruits alleviates high-salt and cholesterol diet-induced cognitive impairment in rats via Nrf2-ARE pathway","volume":"9","author":"Husain","year":"2018","journal-title":"Front Pharmacol."},{"key":"B29","doi-asserted-by":"publisher","first-page":"439","DOI":"10.3390\/biom10030439","article-title":"Tannic acid improves renal function recovery after renal warm ischemia-reperfusion in a rat model","volume":"10","author":"Alechinsky","year":"2020","journal-title":"Biomolecules."},{"key":"B30","doi-asserted-by":"publisher","first-page":"104058","DOI":"10.1016\/j.jff.2020.104058","article-title":"Supplementation with yeast culture improves the integrity of intestinal tight junction proteins via NOD1\/NF-\u03baB P65 pathway in weaned piglets and H2O2-challenged IPEC-J2 cells","volume":"72","author":"Wang","year":"2020","journal-title":"J Funct Foods."},{"key":"B31","doi-asserted-by":"publisher","first-page":"967","DOI":"10.1017\/S0007114510005660","article-title":"Intestinal barrier function and absorption in pigs after weaning: a review","volume":"105","author":"Wijtten","year":"2011","journal-title":"Br J Nutr."},{"key":"B32","doi-asserted-by":"publisher","first-page":"87","DOI":"10.1186\/s40104-020-00496-5","article-title":"Tannic acid prevents post-weaning diarrhea by improving intestinal barrier integrity and function in weaned piglets","volume":"11","author":"Yu","year":"2020","journal-title":"J Anim Sci Biotechnol."},{"key":"B33","doi-asserted-by":"publisher","first-page":"L794","DOI":"10.1152\/ajplung.00322.2005","article-title":"Effects of corticosteroid-induced apoptosis on airway epithelial wound closure in vitro","volume":"291","author":"Dorscheid","year":"2006","journal-title":"Am J Physiol Lung Cell Mol Physiol."},{"key":"B34","doi-asserted-by":"publisher","first-page":"509","DOI":"10.1093\/intimm\/dxu051","article-title":"Pleiotropic functions of TNF-alpha in the regulation of the intestinal epithelial response to inflammation","volume":"26","author":"Leppkes","year":"2014","journal-title":"Int Immunol."},{"key":"B35","doi-asserted-by":"publisher","first-page":"2360","DOI":"10.3390\/ijerph15112360","article-title":"Wound healing properties of selected natural products","volume":"15","author":"Ibrahim","year":"2018","journal-title":"Int J Environ Res Public Health"},{"key":"B36","doi-asserted-by":"publisher","first-page":"2271","DOI":"10.3390\/nu11102271","article-title":"Catechin and procyanidin B-2 modulate the expression of tight junction proteins but do not protect from inflammation-induced changes in permeability in human intestinal cell monolayers","volume":"11","author":"Bianchi","year":"2019","journal-title":"Nutrients."},{"key":"B37","doi-asserted-by":"publisher","first-page":"1603","DOI":"10.1016\/j.freeradbiomed.2010.09.006","article-title":"Oxidative stress, inflammation, and cancer: how are they linked?","volume":"49","author":"Reuter","year":"2010","journal-title":"Free Radic Biol Med."},{"key":"B38","doi-asserted-by":"publisher","first-page":"7432797","DOI":"10.1155\/2016\/7432797","article-title":"Oxidative stress and inflammation: what polyphenols can do for us?","volume":"2016","author":"Hussain","year":"2016","journal-title":"Oxid Med Cell Longev."},{"key":"B39","doi-asserted-by":"publisher","first-page":"179","DOI":"10.1007\/978-981-15-0602-4_9","article-title":"Oxidative stress and autophagy","volume":"1206","author":"Gao","year":"2019","journal-title":"Adv Exp Med Biol."},{"key":"B40","doi-asserted-by":"publisher","first-page":"199","DOI":"10.1016\/j.freeradbiomed.2015.06.014","article-title":"p62 links autophagy and Nrf2 signaling","volume":"88","author":"Jiang","year":"2015","journal-title":"Free Radic Biol Med"}],"container-title":["Frontiers in Nutrition"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fnut.2022.1012207\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,11,4]],"date-time":"2022-11-04T07:02:24Z","timestamp":1667545344000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fnut.2022.1012207\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,11,4]]},"references-count":40,"alternative-id":["10.3389\/fnut.2022.1012207"],"URL":"https:\/\/doi.org\/10.3389\/fnut.2022.1012207","relation":{},"ISSN":["2296-861X"],"issn-type":[{"value":"2296-861X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,11,4]]},"article-number":"1012207"}}