{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T11:48:06Z","timestamp":1791546486450,"version":"4.3.4"},"reference-count":43,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,11,22]],"date-time":"2022-11-22T00:00:00Z","timestamp":1669075200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012514","name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red-Fisiopatolog\u00eda de la Obesidad y Nutrici\u00f3n","doi-asserted-by":"publisher","award":["CB06\/03\/1012"],"award-info":[{"award-number":["CB06\/03\/1012"]}],"id":[{"id":"10.13039\/501100012514","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004837","name":"Ministerio de Ciencia e Innovaci\u00f3n","doi-asserted-by":"publisher","award":["PID2019-104915RB-I00"],"award-info":[{"award-number":["PID2019-104915RB-I00"]}],"id":[{"id":"10.13039\/501100004837","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004837","name":"Ministerio de Ciencia e Innovaci\u00f3n","doi-asserted-by":"publisher","award":["PID2019-109369RB-I00"],"award-info":[{"award-number":["PID2019-109369RB-I00"]}],"id":[{"id":"10.13039\/501100004837","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010067","name":"Gobierno de Arag\u00f3n","doi-asserted-by":"publisher","award":["A03_20R"],"award-info":[{"award-number":["A03_20R"]}],"id":[{"id":"10.13039\/501100010067","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010067","name":"Gobierno de Arag\u00f3n","doi-asserted-by":"publisher","award":["B16_20R"],"award-info":[{"award-number":["B16_20R"]}],"id":[{"id":"10.13039\/501100010067","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010676","name":"H2020 Societal Challenges","doi-asserted-by":"publisher","award":["SFS-2014-2 (635597 - EcoProlive)"],"award-info":[{"award-number":["SFS-2014-2 (635597 - EcoProlive)"]}],"id":[{"id":"10.13039\/100010676","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100011033","name":"Agencia Estatal de Investigaci\u00f3n","doi-asserted-by":"publisher","award":["Juan de la Cierva Grant IJC2020-044353-I\/MCIN\/AEI\/10.13039\/501100011033\/EU\/PRTR"],"award-info":[{"award-number":["Juan de la Cierva Grant IJC2020-044353-I\/MCIN\/AEI\/10.13039\/501100011033\/EU\/PRTR"]}],"id":[{"id":"10.13039\/501100011033","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Nutr."],"abstract":"<jats:sec><jats:title>Introduction<\/jats:title><jats:p>Pulsed electric field (PEF) has been used for improving extraction of extra virgin olive oil (EVOO). However, the biological changes induced by the consumption of pulsed electric field-obtained extra virgin olive oil (PEFEVOO) have not been studied yet.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and methods<\/jats:title><jats:p>EVOO oils from Empeltre variety were prepared by standard (STD) cold pressure method involving crushing of the olives, malaxation and decanting and by this procedure including an additional step of PEF treatment. Chemical analyses of EVOO oils were done. Male and female <jats:italic>Apoe<\/jats:italic>-deficient mice received diets differing in both EVOOs for 12 weeks, and their plasma, aortas and livers were analyzed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>PEF application resulted in a 17% increase in the oil yield and minimal changes in chemical composition regarding phytosterols, phenolic compounds and microRNA. Only in females mice consuming PEF EVOO, a decreased plasma total cholesterol was observed, without significant changes in atherosclerosis and liver steatosis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>PEF technology applied to EVOO extraction maintains the EVOO quality and improves the oil yield. The equivalent biological effects in atherosclerosis and fatty liver disease of PEF-obtained EVOO further support its safe use as a food.<\/jats:p><\/jats:sec>","DOI":"10.3389\/fnut.2022.1065543","type":"journal-article","created":{"date-parts":[[2022,11,22]],"date-time":"2022-11-22T05:49:28Z","timestamp":1669096168000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":20,"title":["Pulsed electric field increases the extraction yield of extra virgin olive oil without loss of its biological properties"],"prefix":"10.3389","volume":"9","author":[{"given":"Roberto","family":"Mart\u00ednez-Beamonte","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marina","family":"Ripalda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tania","family":"Herrero-Continente","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristina","family":"Barranquero","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alberto","family":"D\u00e1valos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda Carmen","family":"L\u00f3pez de las Hazas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ignacio","family":"\u00c1lvarez-Lanzarote","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana Cristina","family":"S\u00e1nchez-Gimeno","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Javier","family":"Raso","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carmen","family":"Arnal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joaqu\u00edn C.","family":"Surra","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jes\u00fas","family":"Osada","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda A.","family":"Navarro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2022,11,22]]},"reference":[{"key":"B1","doi-asserted-by":"crossref","first-page":"903","DOI":"10.1093\/oxfordjournals.aje.a114480","article-title":"The diet and 15-year death rate in the seven countries study.","volume":"124","author":"Keys","year":"1986","journal-title":"Am J Epidemiol."},{"key":"B2","doi-asserted-by":"crossref","first-page":"2599","DOI":"10.1056\/NEJMoa025039","article-title":"Adherence to a Mediterranean diet and survival in a Greek population.","volume":"348","author":"Trichopoulou","year":"2003","journal-title":"N Engl J Med."},{"key":"B3","doi-asserted-by":"publisher","first-page":"549","DOI":"10.1111\/joim.13333","article-title":"The Mediterranean diet and health: a comprehensive overview.","volume":"290","author":"Guasch-Ferr\u00e9","year":"2021","journal-title":"J Intern Med."},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.3390\/nu11092039","article-title":"Virgin olive oil and health: summary of the III international conference on virgin olive oil and health consensus report, JAEN (Spain) 2018.","volume":"11","author":"Gaforio","year":"2019","journal-title":"Nutrients."},{"key":"B5","doi-asserted-by":"crossref","DOI":"10.1056\/NEJMoa1800389","article-title":"Primary prevention of cardiovascular disease with a Mediterranean diet supplemented with extra-virgin olive oil or nuts.","volume":"378","author":"Estruch","year":"2018","journal-title":"N Engl J Med."},{"key":"B6","doi-asserted-by":"publisher","first-page":"1876","DOI":"10.1016\/S0140-6736(22)00122-2","article-title":"Long-term secondary prevention of cardiovascular disease with a Mediterranean diet and a low-fat diet (CORDIOPREV): a randomised controlled trial.","volume":"399","author":"Delgado-Lista","year":"2022","journal-title":"Lancet."},{"key":"B7","doi-asserted-by":"publisher","first-page":"101","DOI":"10.1016\/j.jacc.2021.10.041","article-title":"Consumption of olive oil and risk of total and cause-specific mortality among U.S. adults.","volume":"79","author":"Guasch-Ferr\u00e9","year":"2022","journal-title":"J Am Coll Cardiol."},{"key":"B8","doi-asserted-by":"publisher","first-page":"1043","DOI":"10.1002\/mnfr.201100668","article-title":"Efficacy of bioactive compounds from extra virgin olive oil to modulate atherosclerosis development.","volume":"56","author":"Lou-Bonafonte","year":"2012","journal-title":"Mol Nutr Food Res."},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0261649","article-title":"Olive oil intake and cancer risk: a systematic review and meta-analysis.","volume":"17","author":"Markellos","year":"2022","journal-title":"PLoS One."},{"key":"B10","doi-asserted-by":"publisher","first-page":"2039","DOI":"10.1093\/advances\/nmac067","article-title":"Nutri-epigenetic effects of phenolic compounds from extra virgin olive oil: a systematic review.","volume":"13","author":"Del Saz-Lara","year":"2022","journal-title":"Adv Nutr."},{"key":"B11","doi-asserted-by":"publisher","first-page":"418","DOI":"10.1016\/j.jnutbio.2006.08.005","article-title":"Olive oil preparation determines the atherosclerotic protection in apolipoprotein E knockout mice.","volume":"18","author":"Acin","year":"2007","journal-title":"J Nutr Biochem."},{"key":"B12","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1016\/j.ifset.2018.02.002","article-title":"High power ultrasound frequency for olive paste conditioning: effect on the virgin olive oil bioactive compounds and sensorial characteristics.","volume":"47","author":"Bejaoui","year":"2018","journal-title":"Innov Food Sci Emerg Technol."},{"key":"B13","doi-asserted-by":"crossref","DOI":"10.1016\/j.ifset.2022.103036","article-title":"High vacuum applied during malaxation in oil industrial plant: influence on virgin olive oil extractability and quality.","volume":"79","author":"Veneziani","year":"2022","journal-title":"Innov Food Sci Emerg Technol."},{"key":"B14","doi-asserted-by":"publisher","first-page":"107","DOI":"10.1038\/cr.2011.158","article-title":"Exogenous plant MIR168a specifically targets mammalian LDLRAP1: evidence of cross-kingdom regulation by microRNA.","volume":"22","author":"Zhang","year":"2012","journal-title":"Cell Res."},{"key":"B15","doi-asserted-by":"publisher","first-page":"217","DOI":"10.1038\/cr.2016.13","article-title":"Cross-kingdom inhibition of breast cancer growth by plant miR159.","volume":"26","author":"Chin","year":"2016","journal-title":"Cell Res."},{"key":"B16","doi-asserted-by":"publisher","first-page":"2218","DOI":"10.1111\/bph.15421","article-title":"Eating microRNAs: pharmacological opportunities for cross-kingdom regulation and implications in host gene and gut microbiota modulation.","volume":"178","author":"Del Pozo-Acebo","year":"2021","journal-title":"Br J Pharmacol."},{"key":"B17","doi-asserted-by":"publisher","DOI":"10.1016\/j.phrs.2022.106472","article-title":"Therapeutic potential of broccoli-derived extracellular vesicles as nanocarriers of exogenous miRNAs.","volume":"185","author":"Del Pozo-Acebo","year":"2022","journal-title":"Pharmacol Res."},{"key":"B18","doi-asserted-by":"publisher","DOI":"10.3390\/ijms231911681","article-title":"Variation of miRNA content in cow raw milk depending on the dairy production system.","volume":"23","author":"Abou","year":"2022","journal-title":"Int J Mol Sci."},{"key":"B19","doi-asserted-by":"publisher","first-page":"102","DOI":"10.1007\/s11130-016-0534-9","article-title":"Unsuccessful detection of plant MicroRNAs in beer, extra virgin olive oil and human plasma after an acute ingestion of extra virgin olive oil.","volume":"71","author":"Mico","year":"2016","journal-title":"Plant Foods Hum Nutr."},{"key":"B20","doi-asserted-by":"publisher","DOI":"10.1186\/s43094-022-00413-9","article-title":"Recent trends in miRNA therapeutics and the application of plant miRNA for prevention and treatment of human diseases.","volume":"8","author":"Saiyed","year":"2022","journal-title":"Future J Pharm Sci."},{"key":"B21","first-page":"p. 1","author":"Heinz","year":"2006","journal-title":"Pulsed Electric Fields Technology for the Food Industry: Fundamentals and Applications."},{"key":"B22","first-page":"p. 1","author":"Raso","year":"2022","journal-title":"Pulsed Electric Fields Technology for the Food Industry: Fundamentals and Applications."},{"key":"B23","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1016\/j.jfoodeng.2004.04.029","article-title":"Impact of pulsed electric field treatment on the recovery and quality of plant oils.","volume":"67","author":"Guderjan","year":"2005","journal-title":"J Food Eng."},{"key":"B24","doi-asserted-by":"crossref","first-page":"1367","DOI":"10.1007\/s11947-012-0817-6","article-title":"Effects of pulsed electric field on yield extraction and quality of olive oil.","volume":"6","author":"Abenoza","year":"2013","journal-title":"Food Bioprocess Technol."},{"key":"B25","doi-asserted-by":"publisher","first-page":"497","DOI":"10.1016\/j.foodchem.2014.07.029","article-title":"Olive oil pilot-production assisted by pulsed electric field: impact on extraction yield, chemical parameters and sensory properties.","volume":"167","author":"Puertolas","year":"2015","journal-title":"Food Chem."},{"key":"B26","doi-asserted-by":"crossref","DOI":"10.3389\/fnut.2019.00134","article-title":"Extra-virgin olive oil extracted using pulsed electric field technology: cultivar impact on oil yield and quality.","volume":"6","author":"Veneziani","year":"2019","journal-title":"Front Nutr."},{"key":"B27","doi-asserted-by":"publisher","first-page":"294","DOI":"10.1016\/s1885-5857(09)71560-9","article-title":"Knowledge of the biological actions of extra virgin olive oil gained from mice lacking apolipoprotein E.","volume":"62","author":"Guillen","year":"2009","journal-title":"Rev Esp Cardiol."},{"key":"B28","doi-asserted-by":"publisher","DOI":"10.3390\/nu13061838","article-title":"Dietary avian proteins are comparable to soybean proteins on the atherosclerosis development and fatty liver disease in apoe-deficient mice.","volume":"13","author":"Martinez-Beamonte","year":"2021","journal-title":"Nutrients."},{"key":"B29","first-page":"p. 5","author":"Hermoso","year":"1991","journal-title":"Elaboraci\u00f3n del Aceite de Oliva de Calidad."},{"key":"B30","first-page":"379","article-title":"Informe sobre la utilizaci\u00f3n del analizador de rendimientos \u201cabencor\u201d.","volume":"26","author":"Mart\u00ednez","year":"1975","journal-title":"Grasas Aceites."},{"key":"B31","article-title":"Commission regulation (EEC) No 2568\/91 of 11 July 1991 on the characteristics of olive oil and olive-residue oil and on the relevant methods of analysis.","volume":"34","year":"1991","journal-title":"Off J Eur Comm."},{"key":"B32","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2229-13-10","article-title":"Genome-wide identification of alternate bearing-associated microRNAs (miRNAs) in olive (Olea europaea L.).","volume":"13","author":"Yanik","year":"2013","journal-title":"BMC Plant Biol."},{"key":"B33","doi-asserted-by":"publisher","first-page":"1923","DOI":"10.1093\/jn\/123.11.1923","article-title":"Development and testing of the ain-93 purified diets for rodents \u2013 results on growth, kidney calcification and bone mineralization in rats and mice.","volume":"123","author":"Reeves","year":"1993","journal-title":"J Nutr."},{"key":"B34","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1016\/j.cyto.2005.03.002","article-title":"Immune-regulation of the apolipoprotein A-I\/C-III\/A-IV gene cluster in experimental inflammation.","volume":"31","author":"Navarro","year":"2005","journal-title":"Cytokine."},{"key":"B35","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0104224","article-title":"Dietary squalene increases high density lipoprotein-cholesterol and paraoxonase 1 and decreases oxidative stress in mice.","volume":"9","author":"Gabas-Rivera","year":"2014","journal-title":"PLoS One."},{"key":"B36","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0055231","article-title":"Postprandial changes in high density lipoproteins in rats subjected to gavage administration of virgin olive oil.","volume":"8","author":"Martinez-Beamonte","year":"2013","journal-title":"PLoS One."},{"key":"B37","doi-asserted-by":"crossref","first-page":"1308","DOI":"10.1016\/S0022-2275(20)31582-0","article-title":"A cell-free assay for detecting HDL that is dysfunctional in preventing the formation of or inactivating oxidized phospholipids.","volume":"42","author":"Navab","year":"2001","journal-title":"J Lipid Res."},{"key":"B38","doi-asserted-by":"publisher","first-page":"318","DOI":"10.1016\/j.atherosclerosis.2006.01.015","article-title":"Selective effect of conjugated linoleic acid isomers on atherosclerotic lesion development in apolipoprotein E knockout mice.","volume":"189","author":"Arbones-Mainar","year":"2006","journal-title":"Atherosclerosis."},{"key":"B39","doi-asserted-by":"publisher","first-page":"72","DOI":"10.1016\/j.atherosclerosis.2007.08.008","article-title":"Squalene in a sex-dependent manner modulates atherosclerotic lesion which correlates with hepatic fat content in apoE-knockout male mice.","volume":"197","author":"Guillen","year":"2008","journal-title":"Atherosclerosis."},{"key":"B40","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1016\/B978-0-444-63294-4.00011-5","article-title":"Bioactive components from leaf vegetable products.","volume":"41","author":"Barba","year":"2014","journal-title":"Stud Nat Prod Chem."},{"key":"B41","doi-asserted-by":"crossref","DOI":"10.3390\/foods11172587","article-title":"Authenticity in olive oils from an empeltre clonal selection in Aragon (Spain): how environmental, agronomic, and genetic factors affect sterol composition.","volume":"11","author":"Rey-Gim\u00e9nez","year":"2022","journal-title":"Foods."},{"key":"B42","doi-asserted-by":"crossref","first-page":"346","DOI":"10.1016\/j.atherosclerosis.2013.11.043","article-title":"Plant sterols and plant stanols in the management of dyslipidaemia and prevention of cardiovascular disease.","volume":"232","author":"Gylling","year":"2014","journal-title":"Atherosclerosis."},{"key":"B43","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1016\/j.atherosclerosis.2008.12.009","article-title":"Surrogate markers in clinical trials \u2013 challenges and opportunities.","volume":"206","author":"Duivenvoorden","year":"2009","journal-title":"Atherosclerosis."}],"container-title":["Frontiers in Nutrition"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fnut.2022.1065543\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,11,22]],"date-time":"2022-11-22T05:49:39Z","timestamp":1669096179000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fnut.2022.1065543\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,11,22]]},"references-count":43,"alternative-id":["10.3389\/fnut.2022.1065543"],"URL":"https:\/\/doi.org\/10.3389\/fnut.2022.1065543","relation":{},"ISSN":["2296-861X"],"issn-type":[{"value":"2296-861X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,11,22]]},"article-number":"1065543"}}