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Nutr."],"abstract":"\n The interaction between exogenous microorganisms and the host has received great attention, and finding new probiotics is always the way to improve the health of humans and animals.\n Lactobacillus amylovorus<\/jats:italic>\n (\n L. amylovorus<\/jats:italic>\n ) is a kind of\n Lactobacillus<\/jats:italic>\n that can efficiently utilize starch, as a food and feed additive, it has been widely used for mildew prevention and antibacterial, bacteriostasis, and enzyme production. Herein, a strain of\n L. amylovorus<\/jats:italic>\n was isolated from the feces of Tibetan weaned piglets, named\n L. amylovorus<\/jats:italic>\n SLZX20-1. Physiological and biochemical experiments\n in vitro<\/jats:italic>\n confirmed that it had a fast growth rate and could produce a variety of enzymes, including \u03b1-galactosidase, \u03b2-galactosidase, \u03b1-glucosidase, \u03b2-glucosidase, and ferulic acid esterase. In addition,\n L. amylovorus<\/jats:italic>\n SLZX20-1 exerted antibiotic effects on the growth of\n Salmonella typhimurium<\/jats:italic>\n (\n S. typhimurium<\/jats:italic>\n ) SL1344,\n Citrobacter rodentium<\/jats:italic>\n (\n C. rodentium<\/jats:italic>\n ) DBS100,\n Salmonella pullorum<\/jats:italic>\n (\n S. pullorum<\/jats:italic>\n ) CVCC1791,\n Staphylococcus aureus<\/jats:italic>\n (\n S. aureus<\/jats:italic>\n ) CVCC1882,\n Escherichia coli<\/jats:italic>\n (\n E. coli<\/jats:italic>\n ) O157,\n E. coli<\/jats:italic>\n K88,\n E. coli<\/jats:italic>\n K99, and\n E. coli<\/jats:italic>\n 987P, which are closely related to acid productivity, such as lactic acid and acetic acid.\n In vitro<\/jats:italic>\n co-culture,\n L. amylovorus<\/jats:italic>\n SLZX20-1 has shown the strong adhesion ability to intestinal porcine epithelial cells (IPEC-J2 cells) and activated IPEC-J2 cells with high expression of host defense peptides (HDPs), such as\n NK-Lysin, PEP2C<\/jats:italic>\n , and\n PBD-1<\/jats:italic>\n .\n In vivo<\/jats:italic>\n experiment,\n via<\/jats:italic>\n intragastric administration,\n L. amylovorus<\/jats:italic>\n SLZX20-1 significantly improved the feed intake of mice, declined the crypt depth of jejunum and ileum,\n L. amylovorus<\/jats:italic>\n SLZX20-1 changed the composition of intestinal microbes, especially at the level of colonic genus, the dominant genus was changed from\n Lactobacillus<\/jats:italic>\n to\n S24-7<\/jats:italic>\n , which indicated the change of intestinal carbohydrate nutrition. In conclusion,\n L. amylovorus<\/jats:italic>\n SLZX20-1 showed strong probiotic characteristics, which met with the standard of probiotics and is worth further exploring its impacts on host health and its potential as a candidate strain of probiotics.\n <\/jats:p>","DOI":"10.3389\/fnut.2022.846991","type":"journal-article","created":{"date-parts":[[2022,3,30]],"date-time":"2022-03-30T01:18:40Z","timestamp":1648603120000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":55,"title":["Tibetan Pig-Derived Probiotic Lactobacillus amylovorus SLZX20-1 Improved Intestinal Function via Producing Enzymes and Regulating Intestinal Microflora"],"prefix":"10.3389","volume":"9","author":[{"given":"Jiakun","family":"Shen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jie","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zishen","family":"Lin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linbao","family":"Ji","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xi","family":"Ma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2022,3,29]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"163","DOI":"10.1016\/S1097-2765(00)80412-8","article-title":"Rnq1: an epigenetic modifier of protein function in yeast","volume":"5","author":"Sondheimer","year":"2000","journal-title":"Mol Cell."},{"key":"B2","doi-asserted-by":"publisher","first-page":"6","DOI":"10.3389\/fendo.2013.00006","article-title":"Anabolic effects of Igf-1 signaling on the skeleton","volume":"4","author":"Tahimic","year":"2013","journal-title":"Front Endocrinol."},{"key":"B3","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1080\/10408398.2020.1828813","article-title":"Dietary nutrients shape gut microbes and intestinal mucosa via epigenetic modifications","volume":"20","author":"Wu","year":"2020","journal-title":"Critic Rev Food Sci Nutri."},{"key":"B4","doi-asserted-by":"publisher","first-page":"5157","DOI":"10.1021\/acs.jafc.8b01253","article-title":"Dietary clostridium butyricum induces a phased shift in fecal microbiota structure and increases the acetic acid-producing bacteria in a weaned piglet model","volume":"66","author":"Zhang","year":"2018","journal-title":"J Agric Food Chem."},{"key":"B5","doi-asserted-by":"publisher","first-page":"6","DOI":"10.3390\/nu11061297","article-title":"Lactobacillus acidophilus Dds-1 modulates intestinal-specific microbiota, short-chain fatty acid and immunological profiles in aging mice","volume":"11","author":"Vemuri","year":"2019","journal-title":"Nutrients"},{"key":"B6","doi-asserted-by":"publisher","first-page":"111","DOI":"10.2323\/jgam.53.111","article-title":"Selection of Lactobacillus sp. 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