{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,15]],"date-time":"2025-12-15T19:49:57Z","timestamp":1765828197564,"version":"3.41.2"},"reference-count":36,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,8,31]],"date-time":"2022-08-31T00:00:00Z","timestamp":1661904000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Nutr."],"abstract":"Background<\/jats:title>High carbohydrate, i.e., sugars, intake potentially drives the liver into a lipogenic state leading to elevated plasma fatty acids. Excessive intake of saturated fat and sugar-sweetened soda induces liver fat accumulation, but studying the effect of high intake from sugar-sweetened soda on the de novo<\/jats:italic> lipogenesis (DNL) fatty acids in long-term randomized trials is lacking.<\/jats:p><\/jats:sec>Objective<\/jats:title>To study the effect of consuming 1 L\/day of sugar-sweetened soda, semi-skimmed milk (milk), aspartame-sweetened soda or water over 24 weeks on DNL-derived fatty acids (i.e., palmitate (primary outcome) and other saturated and monounsaturated fatty acids), and markers of stearoyl-CoA desaturase activity (SCD1) in plasma phospholipids (PL), cholesteryl esters (CE), and triglycerides (TG).<\/jats:p><\/jats:sec>Design and methods<\/jats:title>A randomized parallel study was conducted simultaneously at Aarhus University Hospital and Copenhagen University, Denmark, including (n<\/jats:italic> = 41) individuals aged 20\u201350 years, with BMI of 26\u201340 kg\/m2<\/jats:sup>, and without diabetes. The groups consisted of 9 individuals in the sugar-sweetened soda, 10 in the milk, 11 in the aspartame-sweetened soda, and 11 in the water. The change at 24 weeks was assessed and compared across the groups using ANCOVA and mixed-effects models. Correlations of fatty acid changes with liver fat accumulation (magnetic resonance imaging) were explored.<\/jats:p><\/jats:sec>Results<\/jats:title>After 24 weeks, the groups differed in palmitate proportions in PL, oleate in CE and PL, and palmitoleate and SCD1 in all fractions (p<\/jats:italic> &lt; 0.05). Compared with water, the relative proportion of palmitate in PL increased by approximately 1% during both sugar-sweetened soda (p<\/jats:italic> = 0.011) and milk (p<\/jats:italic> = 0.006), whereas oleate and palmitoleate increased only during sugar-sweetened soda (CE 2.77%, p<\/jats:italic> &lt; 0.001; PL 1.51%, p<\/jats:italic> = 0.002 and CE 1.46%, PL 0.24%, TG 1.31%, all p<\/jats:italic> &lt; 0.001, respectively). Liver fat accumulation correlated consistently with changes in palmitoleate, whereas correlations with palmitate and oleate were inconsistent across lipid fractions.<\/jats:p><\/jats:sec>Conclusions<\/jats:title>Although both sugar-sweetened soda and milk increased palmitate in PL, only excess intake of sugar-sweetened soda increased palmitoleate in all lipid fractions and correlated with liver fat. In contrast, isocaloric milk intake did not increase plasma monounsaturated fatty acids.<\/jats:p><\/jats:sec>Clinical trial registration<\/jats:title>[https:\/\/clinicaltrials.gov\/ct2\/show\/NCT00777647<\/jats:ext-link>], identifier [NCT00777647].<\/jats:p><\/jats:sec>","DOI":"10.3389\/fnut.2022.936828","type":"journal-article","created":{"date-parts":[[2022,8,31]],"date-time":"2022-08-31T06:03:10Z","timestamp":1661925790000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":6,"title":["Effects of sugar-sweetened soda on plasma saturated and monounsaturated fatty acids in individuals with obesity: A randomized study"],"prefix":"10.3389","volume":"9","author":[{"given":"Mohammed Fahad","family":"Bajahzer","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jens 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