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Nutr."],"abstract":"<jats:p><jats:italic>Bacillus subtilis<\/jats:italic> can secret a variety of substances to improve human and animal gut health <jats:italic>via<\/jats:italic> inhibiting the proliferation of pathogenic bacteria. In this study, a fast-growing and stress-resistant strain of <jats:italic>Bacillus subtilis M6<\/jats:italic> (<jats:italic>B. subtilis M6<\/jats:italic>) were isolated, which showed a strong antibacterial activity to <jats:italic>E. coli<\/jats:italic> K88, <jats:italic>S. typhimurium<\/jats:italic> ATCC14028, and <jats:italic>S. aureus<\/jats:italic> ATCC25923 <jats:italic>in vitro<\/jats:italic>. <jats:italic>In vivo<\/jats:italic> studies showed that <jats:italic>B. subtilis M6<\/jats:italic> can significantly improve the average daily gain (ADG) using an AA broiler model. Dietary <jats:italic>B. subtilis M6<\/jats:italic> improved the intestinal morphology. The villus height of jejunum and ileum were significantly increased. The concentration of malondialdehyde (MDA) in the ileal mucosa was significantly reduced in <jats:italic>B. subtilis M6<\/jats:italic> treatment group, which suggested the oxidative stress of the ileum was significantly relieved. Though the \u03b2 diversity of treatments was not significantly, <jats:italic>B. subtilis M6<\/jats:italic> improved the composition of intestinal microbes, especially at the level of caecum genus, the dominant genus was changed from <jats:italic>Ruminococcus<\/jats:italic> to <jats:italic>Akkermansia<\/jats:italic>, which indicated the change of intestinal carbohydrate nutrition. In conclusion, these data indicate that the <jats:italic>B. subtilis<\/jats:italic> M6 shows a probiotic potential to improve intestinal health <jats:italic>via<\/jats:italic> altering gut microbiota.<\/jats:p>","DOI":"10.3389\/fnut.2022.965310","type":"journal-article","created":{"date-parts":[[2022,8,17]],"date-time":"2022-08-17T06:42:29Z","timestamp":1660718549000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":30,"title":["Bacillus subtilis M6 improves intestinal barrier, antioxidant capacity and gut microbial composition in AA 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