{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T22:26:37Z","timestamp":1783117597325,"version":"3.54.6"},"reference-count":65,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,10,4]],"date-time":"2022-10-04T00:00:00Z","timestamp":1664841600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100014440","name":"Ministerio de Ciencia, Innovaci\u00f3n y Universidades","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100014440","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Nutr."],"abstract":"<jats:p>This work describes a method for deriving riboflavin overproducing strains of <jats:italic>Weissella cibaria<\/jats:italic> by exposing three strains (BAL3C-5, BAL3C-7, and BAL3C-22) isolated from dough to increasing concentrations of roseoflavin. By this procedure, we selected one mutant overproducing strain from each parental strain (BAL3C-5 B2, BAL3C-7 B2, and BAL3C-22 B2, respectively). Quantification of dextran and riboflavin produced by the parental and mutant strains in a defined medium lacking riboflavin and polysaccharides confirmed that riboflavin was only overproduced by the mutant strains, whereas dextran production was similar in both mutant and parental strains. The molecular basis of the riboflavin overproduction by the mutants was determined by nucleotide sequencing of their <jats:italic>rib<\/jats:italic> operons, which encode the enzymes of the riboflavin biosynthetic pathway. We detected a unique mutation in each of the overproducing strains. These mutations, which map in the sensor domain (aptamer) of a regulatory element (the so-called FMN riboswitch) present in the 5\u2019 untranslated region of the <jats:italic>rib<\/jats:italic> operon mRNA, appear to be responsible for the riboflavin-overproducing phenotype of the BAL3C-5 B2, BAL3C-7 B2, and BAL3C-22 B2 mutant strains. Furthermore, the molecular basis of dextran production by the six <jats:italic>W. cibaria<\/jats:italic> strains has been characterized by (<jats:italic>i<\/jats:italic>) the sequencing of their <jats:italic>dsr<\/jats:italic> genes encoding dextransucrases, which synthesize dextran using sucrose as substrate, and (<jats:italic>ii<\/jats:italic>) the detection of active Dsr proteins by zymograms. Finally, the parental and mutant strains were analyzed for <jats:italic>in situ<\/jats:italic> production of riboflavin and dextran during experimental bread making. The results indicate that the mutant strains were able to produce experimental wheat breads biofortified with both riboflavin and dextran and, therefore, may be useful for the manufacture of functional commercial breads.<\/jats:p>","DOI":"10.3389\/fnut.2022.978831","type":"journal-article","created":{"date-parts":[[2022,10,4]],"date-time":"2022-10-04T06:50:02Z","timestamp":1664866202000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":29,"title":["Weissella cibaria riboflavin-overproducing and dextran-producing strains useful for the development of functional bread"],"prefix":"10.3389","volume":"9","author":[{"given":"Annel M.","family":"Hern\u00e1ndez-Alc\u00e1ntara","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rosana","family":"Chiva","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda Luz","family":"Mohedano","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pasquale","family":"Russo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 \u00c1ngel","family":"Ruiz-Mas\u00f3","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gloria","family":"del Solar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giuseppe","family":"Spano","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mercedes","family":"Tamame","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paloma","family":"L\u00f3pez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2022,10,4]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"135","DOI":"10.5772\/63117","article-title":"Biosynthesis of vitamins by probiotic bacteria.","author":"Gu","year":"2016","journal-title":"Probiotics and prebiotics in human nutrition and health."},{"key":"B2","doi-asserted-by":"publisher","first-page":"279","DOI":"10.1002\/9781118868386.ch17","article-title":"B-Group vitamins production by probiotic lactic acid bacteria","author":"LeBlanc","year":"2015","journal-title":"Biotechnology of lactic acid bacteria novel applications"},{"key":"B3","doi-asserted-by":"publisher","first-page":"3650","DOI":"10.1080\/10408398.2016.1145104","article-title":"Riboflavin and health: A review of recent human research.","volume":"57","author":"Thakur","year":"2017","journal-title":"Crit Rev Food Sci Nutr."},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.2903\/j.efsa.2017.4919","article-title":"Dietary reference values for riboflavin.","volume":"15","author":"Turck","year":"2017","journal-title":"EFSA J."},{"key":"B5","doi-asserted-by":"publisher","first-page":"165","DOI":"10.1016\/B978-0-12-813050-6.00007-3","article-title":"Methods for assessment of vitamin B2.","author":"Sherwood","year":"","journal-title":"Laboratory assessment of vitamin status."},{"key":"B6","first-page":"793","article-title":"Immunomodulatory effect of riboflavin deficiency and enrichment \u2013 Reversible pathological response versus silencing of inflammatory activation.","volume":"66","author":"Mazur-Bialy","year":"2015","journal-title":"J Physiol Pharmacol."},{"key":"B7","author":"Harvard","year":"2022","journal-title":"Chan school of public health\u2019s department of nutrition. 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