{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T04:18:23Z","timestamp":1773807503978,"version":"3.50.1"},"reference-count":38,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,8,29]],"date-time":"2022-08-29T00:00:00Z","timestamp":1661731200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Nutr."],"abstract":"<jats:p>The application of artificial insemination is particularly, owing to which breeder animals are considered an important resource in breeding farms. However, the reproductive performance of roosters typically declines with age, and the economic loss experienced by breeders is attributable to this shortened reproductive lifespan.<jats:italic>Lasia spinosa<\/jats:italic>Thw. (LST) reportedly improved reproductive capacity in male rodents. The objective of this study was to investigate the effects of LST on the reproductive performance of aged roosters. Male Guangxi Partridge chicken (mean weight, 3032.41 \u00b1 34.48 g; age, 500 days;<jats:italic>n<\/jats:italic>= 72) randomly received the following three dietary treatments: LST0 group (a basal diet), LST2 group (a basal diet with 2% LST powder), and LST4 group (a basal diet with 4% LST powder). Computer-aided sperm analysis revealed that dietary LST supplementation significantly improved semen volume, sperm motility, and concentration. Furthermore, the most potent effects were observed in the treatment group with the administration of 2% LST, which significantly improved the weight of the testes. Hematoxylin-eosin staining revealed the increase in diameter of the seminiferous tubule and height of the seminiferous tubule epithelium possibly caused as a result of LST treatment. A significant increase in fructose and glucose concentrations were observed in the testis and seminal plasma; in addition, a significant increase was observed in the \u03b1-glycosidase levels in the testis and spermatozoa. However, the monoaldehyde levels in the spermatozoa appeared to decline significantly. Additionally, the fertility rate increased significantly following 2% LST supplementation. RNA-seq analysis revealed that 34 and 16 unigenes were upregulated and downregulated, respectively, in testicular tissues from roosters that received dietary supplementation of 2% LST. The assigned functions of the unigenes revealed that LST primarily influenced the mechanisms underlying catalytic activity and cellular processes. Kyoto Encyclopedia of Genes and Genomes enrichment analysis suggested that spermatogenesis-related pathways were significantly enriched, including ABC transporters, ribosome biogenesis in eukaryotes, and VEGF, cAMP, and ErbB signaling pathways.<\/jats:p>","DOI":"10.3389\/fnut.2022.994783","type":"journal-article","created":{"date-parts":[[2022,8,29]],"date-time":"2022-08-29T14:37:23Z","timestamp":1661783843000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":9,"title":["Dietary Lasia spinosa Thw. improves reproductive performance of aged roosters"],"prefix":"10.3389","volume":"9","author":[{"given":"Yongxing","family":"Hong","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lang","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xingting","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sile","family":"Wu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jian","family":"Wen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haodong","family":"Sun","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kui","family":"Tian","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoxuan","family":"Jia","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuying","family":"Liao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wanwipa","family":"Suthikrai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Theerawat","family":"Tharasanit","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yangqing","family":"Lu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2022,8,29]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"106573","DOI":"10.1016\/j.domaniend.2020.106573","article-title":"Testicular transcriptome analysis under the dietary inclusion of l-carnitine reveals potential key genes associated with oxidative defense and the semen quality factor in aging roosters","volume":"74","author":"Elokil","year":"2021","journal-title":"Domest Anim Endocrin."},{"key":"B2","doi-asserted-by":"publisher","first-page":"67","DOI":"10.1111\/rda.12804","article-title":"Detecting reproductive system abnormalities of broiler breeder roosters at different ages","volume":"52","author":"Lagares","year":"2017","journal-title":"Reprod Domest Anim."},{"key":"B3","first-page":"59","article-title":"Some nutritional aspects of Lasia spinosa (kohila)","volume":"14","author":"Shefana","year":"2009","journal-title":"Vidyodaya J of Sci."},{"key":"B4","doi-asserted-by":"publisher","first-page":"1","DOI":"10.20959\/wjpr201713-9837","article-title":"Phytochemical and ethno-pharmacological properties of lasia spinosa (kohila): a review","volume":"6","author":"Kankanamge","year":"2017","journal-title":"World J of Pharm Res."},{"key":"B5","doi-asserted-by":"publisher","first-page":"223","DOI":"10.3389\/fnut.2021.775223","article-title":"Dietary Lasia spinosa Thw. improves growth performance in broilers","volume":"8","author":"Zhang","year":"2021","journal-title":"Front Nutr."},{"key":"B6","first-page":"947","article-title":"Effects of Lasia spinosa Thw. and season on plasma leptin and glucose of weaned female murrah \u00d7 swamp buffalo calves","volume":"32","author":"Jintana","year":"2013","journal-title":"Buffalo Bull."},{"key":"B7","doi-asserted-by":"publisher","first-page":"532","DOI":"10.4081\/ijas.2007.s2.532","article-title":"Effects of Lasia spinosa Thw. on growth rate and reproductive hormone of weaned Swamp buffalo and Murrah X Swamp buffalo calves","volume":"6","author":"Suthikrai","year":"2007","journal-title":"Italian J of Anim Sci."},{"key":"B8","doi-asserted-by":"crossref","first-page":"69","DOI":"10.56808\/2985-1130.2457","article-title":"Acute to subchronic toxicity and reproductive effects of aqueous ethanolic extract of rhizomes of lasia spinosa thw. in male rats","volume":"43","author":"Kaewamatawong","year":"2013","journal-title":"Thai J Vet Med."},{"key":"B9","doi-asserted-by":"publisher","first-page":"19","DOI":"10.3382\/ps.0160019","article-title":"The collection of spermatozoa from the domestic fowl and turkey","volume":"16","author":"Burrows","year":"1937","journal-title":"Poul Sci."},{"key":"B10","doi-asserted-by":"publisher","first-page":"1556","DOI":"10.1210\/en.2005-0640","article-title":"Testis structure and function in a non-genetic hyperadipose rat model at prepubertal and adult ages","volume":"147","author":"Franca","year":"2006","journal-title":"Endocrinology."},{"key":"B11","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/s13059-014-0550-8","article-title":"Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2","volume":"15","author":"Love","year":"2014","journal-title":"Genome biol."},{"key":"B12","doi-asserted-by":"publisher","first-page":"425","DOI":"10.1007\/s10815-011-9537-5","article-title":"The effects of male aging on semen quality, sperm DNA fragmentation and chromosomal abnormalities in an infertile population","volume":"28","author":"Brahem","year":"2011","journal-title":"J Assist Reprod Gen."},{"key":"B13","doi-asserted-by":"publisher","first-page":"279","DOI":"10.1093\/geronj\/32.3.279","article-title":"Function and histology of testes from aged Coturnix maintained on different photoperiods","volume":"32","author":"Eroschenko","year":"1977","journal-title":"J Gerontol."},{"key":"B14","doi-asserted-by":"publisher","first-page":"345","DOI":"10.1111\/j.1439-0531.2012.02161.x","article-title":"Relationships between fertility and some parameters in male broiler breeders (body and testicular weight, histology and immunohistochemistry of testes, spermatogenesis and hormonal levels)","volume":"48","author":"Sarabia Fragoso","year":"2013","journal-title":"Reprod Domest Anim."},{"key":"B15","doi-asserted-by":"publisher","first-page":"140","DOI":"10.1016\/j.theriogenology.2019.03.006","article-title":"Dietary Guanidinoacetic acid modulates testicular histology and expression of c-Kit and STRA8 genes in roosters","volume":"130","author":"Nasirikhah","year":"2019","journal-title":"Theriogenology."},{"key":"B16","doi-asserted-by":"publisher","first-page":"345","DOI":"10.1016\/j.tem.2004.07.003","article-title":"Lactate and energy metabolism in male germ cells","volume":"15","author":"Boussouar","year":"2004","journal-title":"Trends Endocrinol Metab."},{"key":"B17","doi-asserted-by":"publisher","first-page":"330","DOI":"10.1038\/nrurol.2012.77","article-title":"Metabolic regulation is important for spermatogenesis","volume":"9","author":"Rato","year":"2012","journal-title":"Nat Rev Urol."},{"key":"B18","doi-asserted-by":"publisher","first-page":"765","DOI":"10.1016\/j.reprotox.2006.07.005","article-title":"Association between exposure to persistent organohalogen pollutants and epididymal and accessory sex gland function: multicentre study in Inuit and European populations","volume":"22","author":"Elzanaty","year":"2006","journal-title":"Reprod Toxicol."},{"key":"B19","doi-asserted-by":"publisher","first-page":"106843","DOI":"10.1016\/j.anireprosci.2021.106843","article-title":"Functional difference of ATP-generating pathways in rooster sperm (Gallus gallus domesticus)","volume":"233","author":"Setiawan","year":"2021","journal-title":"Anim Reprod Sci."},{"key":"B20","doi-asserted-by":"publisher","first-page":"801","DOI":"10.1016\/S0093-691X(01)00609-4","article-title":"Effects of glucose and fructose on motility patterns of dog spermatozoa from fresh ejaculates","volume":"56","author":"Rigau","year":"2001","journal-title":"Theriogenology."},{"key":"B21","doi-asserted-by":"publisher","first-page":"303","DOI":"10.1095\/biolreprod62.2.303","article-title":"Sperm mobility: phenotype in roosters (Gallus domesticus) determinedby concentration of motile sperm and straight line velocity","volume":"62","author":"Froman","year":"2000","journal-title":"BIOL REPROD."},{"key":"B22","doi-asserted-by":"publisher","first-page":"95","DOI":"10.1079\/WPS19790008","article-title":"Prediction of the fertility potential of broiler breeder males","volume":"35","author":"Wilson","year":"1979","journal-title":"World Poultry Sci J."},{"key":"B23","doi-asserted-by":"publisher","first-page":"97","DOI":"10.1080\/00071668608416859","article-title":"Correlation of the fertilizing ability of semen from individual male fowls with sperm motility and ATP content","volume":"27","author":"Wishart","year":"1986","journal-title":"Brit Poultry Sci."},{"key":"B24","doi-asserted-by":"publisher","first-page":"400","DOI":"10.1095\/biolreprod61.2.400","article-title":"Sperm mobility: a primary determinant of fertility in the domestic fowl (Gallus domesticus)","volume":"61","author":"Froman","year":"1999","journal-title":"Biol. Reprod."},{"key":"B25","doi-asserted-by":"publisher","first-page":"134","DOI":"10.1111\/rda.12241","article-title":"Role of sperm velocity variables associated with poultry breed in \u2018last male precedence'","volume":"49","author":"Santiago-Moreno","year":"2014","journal-title":"Reprod. Dom. Anim."},{"key":"B26","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/2049-1891-3-31","article-title":"Effects of soybean isoflavones on reproductive parameters in Chinese mini-pig boars","volume":"3","author":"Yuan","year":"2012","journal-title":"J Anim Sci Biotechnol."},{"key":"B27","doi-asserted-by":"publisher","first-page":"R163","DOI":"10.1530\/REP-13-0178","article-title":"Mitochondria functionality and sperm quality","volume":"146","author":"Amaral","year":"2013","journal-title":"Reproduction."},{"key":"B28","doi-asserted-by":"publisher","first-page":"201","DOI":"10.1530\/jrf.0.1060201","article-title":"Lipid and antioxidant changes in semen of broiler fowl from 25 to 60 weeks of age","volume":"106","author":"Kelso","year":"1996","journal-title":"Reproduction."},{"key":"B29","doi-asserted-by":"publisher","first-page":"36","DOI":"10.1038\/aja.2010.68","article-title":"Apoptosis and DNA damage in human spermatozoa","volume":"13","author":"Aitken","year":"2011","journal-title":"Asian J Androl."},{"key":"B30","doi-asserted-by":"crossref","first-page":"530","DOI":"10.1002\/j.1939-4640.1996.tb01830.x","article-title":"Lipid peroxidation and human sperm motility: protective role of vitamin E","volume":"17","author":"Suleiman","year":"1996","journal-title":"J Androl."},{"key":"B31","doi-asserted-by":"crossref","DOI":"10.1007\/978-981-10-3975-1_4","article-title":"Development and preservation of avian sperm","volume-title":"Avian Reproduction.","author":"Asano","year":"2017"},{"key":"B32","doi-asserted-by":"publisher","first-page":"218","DOI":"10.1038\/nrm2646","article-title":"ABC transporters: the power to change","volume":"10","author":"Rees","year":"2009","journal-title":"Nat Rev Mol Cell Bio."},{"key":"B33","doi-asserted-by":"publisher","first-page":"7476","DOI":"10.1073\/pnas.120159197","article-title":"Multidrug-resistance protein 5 is a multispecific organic anion transporter able to transport nucleotide analogs","volume":"97","author":"Wijnholds","year":"2000","journal-title":"P Natl Acad Sci USA."},{"key":"B34","doi-asserted-by":"publisher","first-page":"7149","DOI":"10.1158\/1078-0432.CCR-06-0564","article-title":"ABCC2 (MRP2, cMOAT) can be localized in the nuclear membrane of ovarian carcinomas and correlates with resistance to cisplatin and clinical outcome","volume":"12","author":"Surowiak","year":"2006","journal-title":"Clin Cancer Res."},{"key":"B35","doi-asserted-by":"publisher","first-page":"e0172478","DOI":"10.1371\/journal.pone.0172478","article-title":"Transcriptome using Illumina sequencing reveals the traits of spermatogenesis and developing testes in Eriocheir sinensis","volume":"12","author":"Li","year":"2017","journal-title":"PLoS One."},{"key":"B36","doi-asserted-by":"publisher","first-page":"1313","DOI":"10.1016\/j.fertnstert.2007.08.030","article-title":"Involvement of vascular endothelial growth factor on spermatogenesis in testis with varicocele","volume":"90","author":"Shiraishi","year":"2008","journal-title":"Fertil Steril."},{"key":"B37","doi-asserted-by":"publisher","first-page":"115","DOI":"10.1016\/S0303-7207(01)00696-7","article-title":"The expanding family of CREB\/CREM transcription factors that are involved with spermatogenesis","volume":"187","author":"Don","year":"2002","journal-title":"Mol Cell Endocrinol."},{"key":"B38","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1038\/cddiscovery.2015.18","article-title":"NRG1 and KITL signal downstream of retinoic acid in the germline to support soma-free syncytial growth of differentiating spermatogonia","volume":"1","author":"Chapman","year":"2015","journal-title":"Cell Death Discov."}],"container-title":["Frontiers in Nutrition"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fnut.2022.994783\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,26]],"date-time":"2023-11-26T08:09:17Z","timestamp":1700986157000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fnut.2022.994783\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,8,29]]},"references-count":38,"alternative-id":["10.3389\/fnut.2022.994783"],"URL":"https:\/\/doi.org\/10.3389\/fnut.2022.994783","relation":{},"ISSN":["2296-861X"],"issn-type":[{"value":"2296-861X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,8,29]]},"article-number":"994783"}}