{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,9]],"date-time":"2026-11-09T06:18:11Z","timestamp":1794205091032,"version":"4.8.0"},"reference-count":28,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2024,6,20]],"date-time":"2024-06-20T00:00:00Z","timestamp":1718841600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Nutr."],"abstract":"<jats:p>When assessing protein quality, a correction needs to be made to take into consideration the availability of the amino acids. This correction is based on the digestibility of the amino acids. It is recommended to use ileal (end of small intestine) digestibility as opposed to faecal digestibility. A correction needs to be made for endogenous (gut sourced as opposed to diet sourced) amino acids to give true digestibility as opposed to apparent digestibility. Also, this correction should be made by correcting the amino acid composition for individual amino acid digestibilities as opposed to correcting all amino acids for nitrogen digestibility. Determination of true ileal amino acid digestibility requires the collection of ileal digesta. In the human there are two methods that can be used; naso-ileal intubation and using the ileostomy model. Both are discussed in detail and it is concluded that both are appropriate methods to collect ileal digesta.<\/jats:p>","DOI":"10.3389\/fnut.2024.1407604","type":"journal-article","created":{"date-parts":[[2024,6,20]],"date-time":"2024-06-20T08:59:01Z","timestamp":1718873941000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":12,"title":["Protein and amino acid digestibility: definitions and conventional oro-ileal determination in humans"],"prefix":"10.3389","volume":"11","author":[{"given":"Suzanne M.","family":"Hodgkinson","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2024,6,20]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"S333","DOI":"10.1017\/S0007114512002541","article-title":"Advantages and limitations of the protein digestibility-corrected amino acid score (PDCAAS) as a method for evaluating protein quality in human diets","volume":"108","author":"Schaafsma","year":"2012","journal-title":"Br J Nutr"},{"key":"ref2","first-page":"112","article-title":"Role of the large intestine in the processes of digestion and absorption in the pig","volume-title":"Current concepts of digestion and absorption in pigs","author":"Mason","year":"1980"},{"key":"ref3","doi-asserted-by":"publisher","first-page":"739","DOI":"10.1079\/BJN19940181","article-title":"Absorption of lysine and methionine from the proximal colon of the piglet","volume":"71","author":"Darragh","year":"1994","journal-title":"Br J Nutr"},{"key":"ref4","doi-asserted-by":"publisher","first-page":"29","DOI":"10.1079\/BJN19940108","article-title":"Comparison of the ileal and faecal digestibility of dietary amino acids in adult humans and evaluation of the pig as a model animal for digestion studies in man","volume":"71","author":"Rowan","year":"1994","journal-title":"Br J Nutr"},{"key":"ref5","doi-asserted-by":"publisher","first-page":"1493","DOI":"10.3945\/jn.117.248187","article-title":"Amino acid absorption in the large intestine of humans and porcine models","volume":"147","author":"van der Wielen","year":"2017","journal-title":"J Nutr"},{"key":"ref6","doi-asserted-by":"publisher","first-page":"1857S","DOI":"10.1093\/jn\/130.7.1857S","article-title":"Contribution of microbial amino acids to amino acid homeostasis of the host","volume":"130","author":"Metges","year":"2000","journal-title":"J Nutr"},{"key":"ref7","first-page":"147","article-title":"Endogenous nitrogen losses during digestion in pigs","volume-title":"Proceedings of the Vth international symposium on digestive physiology in pigs","author":"Souffrant","year":"1991"},{"key":"ref8","volume-title":"Dietary protein quality evaluation in human nutrition. 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