{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,22]],"date-time":"2026-01-22T06:16:52Z","timestamp":1769062612740,"version":"3.49.0"},"reference-count":34,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,1,21]],"date-time":"2026-01-21T00:00:00Z","timestamp":1768953600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Nutr."],"abstract":"<jats:p>\n                    Patients with irritable bowel syndrome experience abdominal pain and stool habit disturbances, and often also extraintestinal symptoms, such as fatigue. The disorder is linked to gut dysbiosis, and manipulation of the microbiota is considered a possible treatment strategy. This randomised, double-blinded, placebo-controlled study aimed to investigate the effects of the probiotic strain\n                    <jats:italic>Bifidobacterium breve<\/jats:italic>\n                    , Bif195\u2122 (DSM 33360) (Bif195), on symptoms and gut microbiome composition in patients with diarrhoea-predominant irritable bowel syndrome. Sixty-one patients with moderate\u2013severe disease activity were allocated to 8\u202fweeks of treatment with either Bif195 or placebo (1:1), followed by 8\u202fweeks of follow-up. The primary outcome was a change in symptom scores measured by the validated questionnaire, IBS-symptom severity scale. Secondary and explorative outcomes were the effects of Bif195 on intestinal symptoms, quality of life, fatigue, and the gut microbiota. Modulation of the transepithelial electrical resistance (TEER) of Caco-2 cells by Bif195 was investigated\n                    <jats:italic>in vitro<\/jats:italic>\n                    as a model of barrier integrity. The results showed no effect of Bif195 on primary or secondary outcomes; however, Bif195 lowered fatigue scores compared to placebo. Significantly increased TEER readings\n                    <jats:italic>in vitro<\/jats:italic>\n                    indicated enhanced barrier integrity, suggesting GI permeability as a mechanism for further clinical exploration.\n                  <\/jats:p>\n                  <jats:sec>\n                    <jats:title>Clinical trial registration<\/jats:title>\n                    <jats:p>\n                      <jats:uri>clinicaltrials.gov<\/jats:uri>\n                      , identifier NCT04808271.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fnut.2025.1701341","type":"journal-article","created":{"date-parts":[[2026,1,21]],"date-time":"2026-01-21T14:28:36Z","timestamp":1769005716000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Eight weeks of treatment with probiotic Bifidobacterium breve, Bif195 lowers fatigue scores in patients with diarrhoea-predominant irritable bowel syndrome: results from a randomised, clinical trial"],"prefix":"10.3389","volume":"12","author":[{"given":"Ida Marie Bruun","family":"Gr\u00f8nb\u00e6k","sequence":"first","affiliation":[{"name":"Gastrounit, Medical Section, Copenhagen University Hospital \u2013 Amager and Hvidovre","place":["Hvidovre, Denmark"]},{"name":"Department of Clinical Microbiology, Copenhagen University Hospital \u2013 Amager and Hvidovre","place":["Hvidovre, Denmark"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sofie Ingdam","family":"Halkj\u00e6r","sequence":"additional","affiliation":[{"name":"Gastrounit, Medical Section, Copenhagen University Hospital \u2013 Amager and Hvidovre","place":["Hvidovre, Denmark"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Esben Holm","family":"Hansen","sequence":"additional","affiliation":[{"name":"Gastrounit, Medical Section, Copenhagen University Hospital \u2013 Amager and Hvidovre","place":["Hvidovre, Denmark"]},{"name":"Department of Clinical Medicine, University of Copenhagen","place":["Copenhagen, Denmark"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sarah","family":"Mollerup","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology, Copenhagen University Hospital \u2013 Amager and Hvidovre","place":["Hvidovre, Denmark"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sarah Juel","family":"Paulsen","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology, Copenhagen University Hospital \u2013 Amager and Hvidovre","place":["Hvidovre, Denmark"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christina Valbirk","family":"Konrad","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology, Copenhagen University Hospital \u2013 Amager and Hvidovre","place":["Hvidovre, Denmark"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sara","family":"Engel","sequence":"additional","affiliation":[{"name":"Chr. Hansen A\/S, Part of Novonesis, Human Health Biosolutions","place":["H\u00f8rsholm, Denmark"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Magdalena","family":"Bulinska-Balas","sequence":"additional","affiliation":[{"name":"Chr. Hansen A\/S, Part of Novonesis, Human Health Biosolutions","place":["H\u00f8rsholm, Denmark"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anja","family":"Wellejus","sequence":"additional","affiliation":[{"name":"Chr. Hansen A\/S, Part of Novonesis, Human Health Biosolutions","place":["H\u00f8rsholm, Denmark"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anne Birgitte","family":"Haaber","sequence":"additional","affiliation":[{"name":"Mavespecialisten","place":["Charlottenlund, Denmark"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alice H\u00f8jer","family":"Christensen","sequence":"additional","affiliation":[{"name":"Aleris Hospitals Copenhagen","place":["S\u00f8borg, Denmark"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anne Line","family":"Engsbro","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology, Zealand University Hospital","place":["Slagelse, Denmark"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andreas Munk","family":"Petersen","sequence":"additional","affiliation":[{"name":"Gastrounit, Medical Section, Copenhagen University Hospital \u2013 Amager and Hvidovre","place":["Hvidovre, Denmark"]},{"name":"Department of Clinical Microbiology, Copenhagen University Hospital \u2013 Amager and Hvidovre","place":["Hvidovre, Denmark"]},{"name":"Department of Clinical Medicine, University of Copenhagen","place":["Copenhagen, Denmark"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2026,1,21]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"908","DOI":"10.1016\/s2468-1253(20)30217-x","article-title":"Global prevalence of irritable bowel syndrome according to Rome III or IV criteria: a systematic review and meta-analysis","volume":"5","author":"Oka","year":"2020","journal-title":"Lancet Gastroenterol Hepatol"},{"key":"ref2","doi-asserted-by":"publisher","first-page":"17562848221114558","DOI":"10.1177\/17562848221114558","article-title":"Extraintestinal manifestations in irritable bowel syndrome: a systematic review","volume":"15","author":"Ohlsson","year":"2022","journal-title":"Ther Adv 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