{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,23]],"date-time":"2026-07-23T03:05:56Z","timestamp":1784775956590,"version":"3.55.0"},"reference-count":41,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,1,21]],"date-time":"2026-01-21T00:00:00Z","timestamp":1768953600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Nutr."],"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Autism spectrum disorder (ASD), a neurodevelopmental condition strongly associated with PTEN mutations, demonstrates limited responsiveness to current therapeutic approaches. Ketogenic diet (KD) has emerged as a promising dietary intervention in neurological disorders, including epilepsy and ASD.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>This case report investigates the clinical efficacy and potential mechanisms of KD in an ASD patient with a PTEN mutation, providing evidence for genetics-guided precision nutrition therapy.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We present a 7-year, 1-month-old male with ASD and a pathogenic heterozygous PTEN p. Arg130Gln mutation, who showed suboptimal response to conventional treatments. He received a modified Atkins diet (60% fat, 30% protein, 10% carbohydrates) for 1\u202fmonth, followed by 5\u202fmonths of combined KD and repetitive transcranial magnetic stimulation (rTMS). Efficacy was evaluated through serial behavioral assessments, metabolic markers (blood ketones, inflammatory cytokines), and EEG monitoring. Safety parameters were documented.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>During the intervention, mean blood glucose was 4.4\u202fmmol\/L and ketones averaged 2.4\u202fmmol\/L. Significant improvements in behavior, sleep, and metabolic profiles were observed. Parents noted symptomatic improvement by day 6. Quantitative assessments (behavioral scales, metabolic markers, EEG) confirmed substantial progress after 1\u202fmonth of KD and 5\u202fmonths of KD-rTMS versus baseline. While the rate of improvement diminished during combined therapy compared to initial KD monotherapy, excitatory behaviors (e.g., screaming, uncontrolled running, sleep-onset difficulties) were markedly reduced compared to prior rTMS-only treatment. No adverse events (e.g., hypoglycemia, ketoacidosis) occurred, and the regimen was well-tolerated.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>This study provides preliminary clinical evidence for KD in PTEN-related ASD, suggesting potential modulation of the PI3K\/AKT\/mTOR pathway and neuroinflammation. Although limited by its single-case design and short duration, it offers a novel therapeutic approach for PTEN-mutant ASD. Multicenter randomized controlled trials are warranted to validate efficacy and safety.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fnut.2026.1721018","type":"journal-article","created":{"date-parts":[[2026,1,21]],"date-time":"2026-01-21T05:36:47Z","timestamp":1768973807000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":1,"title":["A Case Report: Effects of a ketogenic diet on PTEN mutation-associated autism spectrum disorder"],"prefix":"10.3389","volume":"13","author":[{"given":"Yu","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Pediatrics, Qilu Hospital of Shandong University, Jinan","place":["Shandong, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanshuo","family":"Guo","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Qilu Hospital of Shandong University, Jinan","place":["Shandong, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mengna","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Qilu Hospital of Shandong University, Jinan","place":["Shandong, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linlin","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Qilu Hospital of Shandong University, Jinan","place":["Shandong, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lihong","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Qilu Hospital of Shandong University, Jinan","place":["Shandong, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiyue","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Qilu Hospital of Shandong University, Jinan","place":["Shandong, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongheng","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Qilu Hospital of Shandong University, Jinan","place":["Shandong, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zihan","family":"Yan","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Qilu Hospital of Shandong University, Jinan","place":["Shandong, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiayi","family":"Gao","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Qilu Hospital of Shandong University, Jinan","place":["Shandong, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aihua","family":"Cao","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Qilu Hospital of Shandong University, Jinan","place":["Shandong, China"]},{"name":"Department of Pediatrics, Qilu Hospital of Shandong University Dezhou Hospital, Dezhou","place":["Shandong, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,1,21]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"157","DOI":"10.1001\/jama.2022.23661","article-title":"Autism spectrum disorder: a review","volume":"329","author":"Hirota","year":"2023","journal-title":"JAMA"},{"key":"ref2","doi-asserted-by":"publisher","first-page":"1","DOI":"10.15585\/mmwr.ss7402a1","article-title":"Prevalence and early identification of autism Spectrum disorder among children aged 4 and 8 years - autism and developmental disabilities monitoring network, 16 sites, United States, 2022","volume":"74","author":"Shaw","year":"2025","journal-title":"MMWR Surveill Summ"},{"key":"ref3","first-page":"245","article-title":"Innate immune dysfunction and neuroinflammation in autism spectrum disorder (ASD)","volume-title":"Brain Behav 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